Colorimetric Logic Gates Based on Ion-Dependent DNAzymes

Two kinds of ion-dependent DNAzymes and tailored substrates enable the design of the logic gates (OR, AND, INHIBIT) using Pb2+ and Cu2+ as inputs; then, a three-input AND logic gate is developed utilizing the unique feature that Hg2+ ions interact with the thymine–thymine (T–T). At first, the substrates are blocked by ion-dependent DNAzymes that include the horseradish peroxidase (HRP)-mimicking DNAzyme sequence. The released product strands then self-assemble into the hemin/G-quadruplex-HRP-mimicking DNAzyme that biocatalyze the formation of a colored product and provide an output signal for the different logic gates. We are able to recognize the logic output signals effortlessly by our naked eyes. It is a simple, economic, and safe approach for the design of a complex multiple-input DNA logic molecular device.

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