IN VITRO COLCHICINE TREATMENT OF ANTHER-DERIVED PEPPER HAPLOIDS

OLSZEWSKA, D., I. JEDRZEJCZYK, P. NOWACZYK, S. SENDEL and B. GACZKOWSKA, 2015. In vitro colchicine treatment of anther-derived pepper haploids. Bulg. J. Agric. Sci., 21: 806–810 Capsicum genus covers several dozens of species, which can be used in crossbreeding with annual pepper. The induction of haploid and dihaploid plants through anther cultures of the received hybrids is one of the possibilities of the fast stabilization of the new variability. In this study the following interspecific hybrids were the plant material for the induction of androgenesis: (905 × ‘Sono’)F1, (905 × ‘Mino’)F1, (405 × ‘Luba’)F1, (405 × ‘Sono’)F1. Haploid plants obtained from anther cultures were colchicine treatment according to protocol developed for Capsicum annuum L. The apical parts of 106 androgenic haploids were placed for six days on the MS medium containing colchicine at the concentration equal 400 mg dm-3. The largest groups of regenerants were mixoploids 29-55% and haploids 20-50%. For the studied genotypes an effective diploidization at the level of 17-27% was observed. Micropropagation was performed on the 23 haploids obtained and the received microcuttings (in the number of 73) underwent again a-six-day and a-nine-day treatment with colchicine on the MS medium containing 200 mg dm-3 of colchicine. The effectiveness of the regeneration after the second colchicine treatment was significantly reduced. Single diploid plants appeared among the regenerants (905 × ‘Sono’)F1 and (405 × ‘Luba’)F1.

[1]  A. Kisiala,et al.  Study of in vitro anther culture in selected genotypes of genus Capsicum , 2014 .

[2]  W. Kang,et al.  In vitro androgenic response of minipaprika (Capsicum annuum L.) genotypes in different culture media , 2013, Horticulture, Environment, and Biotechnology.

[3]  A. Kisiala,et al.  Study of individual plant responsiveness in anther cultures of selected pepper (Capsicum spp.) genotypes , 2012 .

[4]  J. Pauk,et al.  Androgenesis induction in microspore culture of sweet pepper (Capsicum annuum L.) , 2011, Plant Biotechnology Reports.

[5]  S. Kintzios,et al.  Engineering of the membrane of fibroblast cells with virus-specific antibodies: A novel biosensor tool for virus detection. , 2008, Biosensors & bioelectronics.

[6]  A. Kisiala,et al.  The assessment of doubled haploid lines obtained in pepper (Capsicum annuum L.) anther culture , 2011 .

[7]  J. M. Seguí-Simarro,et al.  Androgenesis in recalcitrant solanaceous crops , 2011, Plant Cell Reports.

[8]  L. Leus,et al.  Mitotic chromosome doubling of plant tissues in vitro , 2010, Plant Cell, Tissue and Organ Culture (PCTOC).

[9]  J. Pauk,et al.  In vitro anther and isolated microspore culture as tools in sweet and spice pepper breeding. , 2009 .

[10]  D. Olszewska,et al.  Androgenic response of genotypes selected from Capsicum annuum L. × C. chinense Jacq. hybrids , 2009, Acta Physiologiae Plantarum.

[11]  J. Pauk,et al.  Improvement of isolated microspore culture of pepper (Capsicum annuum L.) via co-culture with ovary tissues of pepper or wheat , 2009, Plant Cell, Tissue and Organ Culture (PCTOC).

[12]  A. Kisiala,et al.  Individual reaction of Capsicum F2 hybrid genotypes in anther cultures , 2009, Euphytica.

[13]  I. Jedrzejczyk,et al.  In vivo polyembryony induction in species of Capsicum genus. , 2009 .

[14]  P. Vági,et al.  Production of doubled haploid breeding lines in case of paprika, spice paprika, eggplant, cucumber, zucchini and onion , 2006 .

[15]  S. Suharsono,et al.  Evaluation of crucial factors for implementing shed-microspore culture of Indonesian hot pepper (Capsicum annuum L.) cultivars , 2006 .

[16]  A. Kisiala,et al.  Induced androgenesis of Capsicum frutescens L. , 2006, Acta Physiologiae Plantarum.

[17]  O. Kumar,et al.  Cytomorphology of induced octoploid Chili pepper (Capsicum annuum L.) , 1984, Theoretical and Applied Genetics.

[18]  G. Gyulai,et al.  Genetic variability of anther donor versus spontaneous doubled haploid descendents and colchicine induced doubled haploid sweet pepper (Capsicum annuum L.) lines , 2001 .

[19]  M. Fári,et al.  PROBLEMS AND RESULTS OF DOUBLED HAPLOID PLANT PRODUCTION IN PEPPER (CAPSICUM ANNUUM L.) VIA ANTHER- AND MICROSPORE CULTURE , 1997 .

[20]  F. Skoog,et al.  A revised medium for rapid growth and bio assays with tobacco tissue cultures , 1962 .