A DNA barcode for land plants

DNA barcoding involves sequencing a standard region of DNA as a tool for species identification. However, there has been no agreement on which region(s) should be used for barcoding land plants. To provide a community recommendation on a standard plant barcode, we have compared the performance of 7 leading candidate plastid DNA regions (atpF–atpH spacer, matK gene, rbcL gene, rpoB gene, rpoC1 gene, psbK–psbI spacer, and trnH–psbA spacer). Based on assessments of recoverability, sequence quality, and levels of species discrimination, we recommend the 2-locus combination of rbcL+matK as the plant barcode. This core 2-locus barcode will provide a universal framework for the routine use of DNA sequence data to identify specimens and contribute toward the discovery of overlooked species of land plants.

W. John Kress | Sujeevan Ratnasingham | John L. Spouge | Mehrdad Hajibabaei | Mark W. Chase | Kenneth M. Cameron | David G. Long | Damon P. Little | Steven G. Newmaster | Vincent Savolainen | Ole Seberg | David L. Erickson | Dion S. Devey | Gitte Petersen | Alexandra Clark | Olivier Maurin | Peter M. Hollingsworth | W. Kress | J. Spouge | M. Chase | G. Petersen | O. Seberg | V. Savolainen | L. Forrest | P. Hollingsworth | S. Graham | S. Ratnasingham | M. VAN DER BANK | Michael Wilkinson | R. Cowan | D. Erickson | S. Madriñán | K. Cameron | M. Carine | T. Hedderson | F. Conrad | G. Salazar | J. Richardson | M. Hollingsworth | L. J. Kelly | D. Long | Mehrdad Hajibabaei | A. Fazekas | K. James | Ki-Joong Kim | H. Schneider | Jonathan van AlphenStahl | S. Barrett | C. van den Berg | D. Bogarín | K. Burgess | J. Chacón | Alexandra Clark | J. J. Clarkson | C. Ford | B. Husband | P. Kesanakurti | Jung Sung Kim | Young-Dong Kim | R. Lahaye | Hae-Lim Lee | O. Maurin | I. Meusnier | S. Newmaster | Chongh-Wook Park | Diana M. Percy | Dong-Keun Yi | D. Little | Harald Schneider | Sean W. Graham | Caroline S. Ford | James E. Richardson | Robyn S. Cowan | Michelle van der Bank | Young-Dong Kim | Kevin S. Burgess | Laura L. Forrest | Aron J. Fazekas | Karen E. James | Ki-Joong Kim | Jonathan van AlphenStahl | Spencer C.H. Barrett | Cassio van den Berg | Diego Bogarin | Mark Carine | Juliana Chacón | James J. Clarkson | Ferozah Conrad | Terry A.J. Hedderson | Michelle L. Hollingsworth | Brian C. Husband | Laura J. Kelly | Prasad R. Kesanakurti | Renaud Lahaye | Hae-Lim Lee | Santiago Madriñán | Isabelle Meusnier | Chong-Wook Park | Gerardo A. Salazar | Michael J. Wilkinson | Dong-Keun Yi | Harald Schneider

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