Determination of leucomalachite green in fish using a novel MIP-coated QDs probe based on synchronous fluorescence quenching effect

Abstract A novel fluorescent probe based on CdTe QDs coated with molecularly imprinted polymers (MIP) was prepared for the determination of leucomalachite green (LMG) with the method of synchronous fluorescence quenching. When the wavelength interval of excitation and emission positions (Δl) was set as 340 nm, the strongest synchronous fluorescence emission of MIP-coated QDs was achieved at 260 nm, enabling the maximum fluorescent quenching of LMG because of the inner filter effect. According to this principle, a synchronous fluorescence quenching method for the rapid detection of LMG in fish was developed. MIP-coated QDs were synthesized using acrylamide (AM) and ethylene glycol dimethacrylate (EGDMA) as functional monomers and cross-linker, respectively. MIP-coated QDs with an average diameter around 67 nm were obtained at the optimum conditions (the molar ratio of 1:8:20 of LMG, AM and EGDMA, 60 mL of acetonitrile, 28 h of polymerization time). The synchronous fluorescence of MIP-coated QDs was linearly quenched by LMG at concentrations from 0.1 to 20 μmol L −1 . Detection limit was 30 nmol L −1 (3 σ , n = 9) with spiked recoveries from 94.0% to 107.8% in fish samples. The results indicate that the method can be applied to the accurate and rapid detection of LMG residues in fish samples.

[1]  M. de la Guardia,et al.  Cocaine abuse determination by ion mobility spectrometry using molecular imprinting. , 2017, Journal of chromatography. A.

[2]  Wei Wang,et al.  In-situ hydrothermal synthesis of molecularly imprinted polymers coated carbon dots for fluorescent detection of bisphenol A , 2016 .

[3]  G. Shi,et al.  Pb2+-modified graphene quantum dots as a fluorescent probe for biological aminothiols mediated by an inner filter effect , 2016 .

[4]  J. Sádecká,et al.  Simultaneous determination of caffeine, caramel and riboflavin in cola-type and energy drinks by synchronous fluorescence technique coupled with partial least squares. , 2014, Food chemistry.

[5]  S. J. Culp,et al.  Malachite Green: A Toxicological Review , 1996 .

[6]  S. J. Culp,et al.  Mutagenicity and carcinogenicity in relation to DNA adduct formation in rats fed leucomalachite green. , 2002, Mutation research.

[7]  Zheng-zhong Lin,et al.  Rapid determination of malachite green in water and fish using a fluorescent probe based on CdTe quantum dots coated with molecularly imprinted polymer , 2017 .

[8]  Cuicui Liu,et al.  Molecularly imprinted polymer on ionic liquid-modified CdSe/ZnS quantum dots for the highly selective and sensitive optosensing of tocopherol , 2012 .

[9]  Jian Deng,et al.  Determination of leucomalachite green, leucocrystal violet and their chromic forms using excitation-emission matrix fluorescence coupled with second-order calibration after dispersive liquid-liquid microextraction. , 2015, Food chemistry.

[10]  Zheng-zhong Lin,et al.  Molecularly imprinted polymers for extraction of malachite green from fish samples prior to its determination by HPLC , 2015, Microchimica Acta.

[11]  Barbara A Rasco,et al.  Rapid analysis of malachite green and leucomalachite green in fish muscles with surface-enhanced resonance Raman scattering. , 2015, Food chemistry.

[12]  Bo Yuan,et al.  A novel recycling system for nano-magnetic molecular imprinting immobilised cellulases: Synergistic recovery of anthocyanin from fruit and vegetable waste. , 2016, Bioresource technology.

[13]  S. J. Culp,et al.  Analysis of mutations and bone marrow micronuclei in Big Blue rats fed leucomalachite green. , 2004, Mutation research.

[14]  S. Dong,et al.  Design of fluorescent assays for cyanide and hydrogen peroxide based on the inner filter effect of metal nanoparticles. , 2009, Analytical chemistry.

[15]  Xiwen He,et al.  Highly sensitive synchronous fluorescence determination of mercury (II) based on the denatured ovalbumin coated CdTe QDs. , 2012, Talanta.

[16]  O. Devos,et al.  Correction of inner filter effect in mirror coating cells for trace level fluorescence measurements. , 2003, Analytical chemistry.

[17]  Feng Liu,et al.  Copper ion-selective fluorescent sensor based on the inner filter effect using a spiropyran derivative. , 2005, Analytical chemistry.

[18]  Yingxin Ma,et al.  Luminescent molecularly-imprinted polymer nanocomposites for sensitive detection , 2015 .

[19]  D. Ribeiro,et al.  Application of nanocrystalline CdTe quantum dots in chemical analysis: Implementation of chemo-sensing schemes based on analyte-triggered photoluminescence modulation , 2017 .

[20]  J. Jaiswal,et al.  Potentials and pitfalls of fluorescent quantum dots for biological imaging. , 2004, Trends in cell biology.

[21]  J. Zhan,et al.  Facile synthesis of N-acetyl-L-cysteine capped ZnS quantum dots as an eco-friendly fluorescence sensor for Hg2+. , 2011, Talanta.

[22]  S. J. Culp,et al.  Toxicity and metabolism of malachite green and leucomalachite green during short-term feeding to Fischer 344 rats and B6C3F1 mice. , 1999, Chemico-biological interactions.

[23]  X. Ren,et al.  Sensitive arginine sensing based on inner filter effect of Au nanoparticles on the fluorescence of CdTe quantum dots. , 2017, Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy.

[24]  M. Smyth,et al.  Confirmatory analysis of malachite green, leucomalachite green, crystal violet and leucocrystal violet in salmon by liquid chromatography-tandem mass spectrometry. , 2007, Analytica Chimica Acta.

[25]  Lun Wang,et al.  Synchronous fluorescence determination of mercury ion with glutathione-capped CdS nanoparticles as a fluorescence probe. , 2010, Talanta.

[26]  S. A. Nsibande,et al.  Fluorescence detection of pesticides using quantum dot materials - A review. , 2016, Analytica chimica acta.

[27]  I. Goryacheva,et al.  Chemometric analysis of luminescent quantum dots systems: Long way to go but first steps taken , 2016 .

[28]  G. O’Connor,et al.  High accuracy determination of malachite green and leucomalachite green in salmon tissue by exact matching isotope dilution mass spectrometry. , 2008, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[29]  S. Yao,et al.  Upconversion nanosensor for sensitive fluorescence detection of Sudan I-IV based on inner filter effect. , 2016, Talanta.

[30]  Jérémy E. Cohen,et al.  Correcting inner filter effects, a non multilinear tensor decomposition method , 2016 .

[31]  D. Shan,et al.  Ethylenediamine-assisted hydrothermal synthesis of nitrogen-doped carbon quantum dots as fluorescent probes for sensitive biosensing and bioimaging , 2015 .

[32]  Xiaoli Hu,et al.  Fluorescent carbon dots for glyphosate determination based on fluorescence resonance energy transfer and logic gate operation , 2017 .

[33]  Wei Zhang,et al.  Composite of CdTe quantum dots and molecularly imprinted polymer as a sensing material for cytochrome c. , 2011, Biosensors & bioelectronics.

[34]  Rutao Liu,et al.  Spectroscopic investigations on the effect of N-acetyl-L-cysteine-capped CdTe Quantum Dots on catalase. , 2014, Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy.

[35]  S. Nie,et al.  Luminescent quantum dots for multiplexed biological detection and imaging. , 2002, Current opinion in biotechnology.