Human TAFII105 Is a Cell Type–Specific TFIID Subunit Related to hTAFII130

We previously characterized Drosophila and human TAF subunits that make up the core TFIID complex found in all cells. Here, we report that differentiated B cells contain a novel substoichiometric TAF of 105 kDa not found associated with TFIID isolated from other cell types. The cDNA encoding hTAFII105 reveals a highly conserved C-terminal domain shared by hTAFII130 and oTAFII110, while the N-terminal coactivator domain has diverged significantly. All cells tested express TAFII105 mRNA, but only B cells contain significant levels of protein associated with TFIID. Transient overexpression of hTAFII105 selectively squelches the transcription of some genes in B cells. These properties suggest that TAFII105 is a cell type-specific subunit of TFIID that may be responsible for mediating transcription by a subset of activators in B cells.

[1]  R. Tjian,et al.  p53 transcriptional activation mediated by coactivators TAFII40 and TAFII60. , 1995, Science.

[2]  X. Jacq,et al.  Human TAFII30 is present in a distinct TFIID complex and is required for transcriptional activation by the estrogen receptor , 1994, Cell.

[3]  D. Reinberg,et al.  Protein-protein interactions in eukaryotic transcription initiation: structure of the preinitiation complex. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[4]  R. Tjian,et al.  Molecular cloning and functional analysis of Drosophila TAF110 reveal properties expected of coactivators , 1993, Cell.

[5]  R. Roeder,et al.  Cloning of an intrinsic human TFIID subunit that interacts with multiple transcriptional activators , 1995, Science.

[6]  Robert Tjian,et al.  Mechanism of transcriptional activation by Sp1: Evidence for coactivators , 1990, Cell.

[7]  Qiang Zhou,et al.  Holo-TFIID supports transcriptional stimulation by diverse activators and from a TATA-less promoter. , 1992, Genes & development.

[8]  S. Burley,et al.  Crystal structure of a TFIIB–TBP–TATA-element ternary complex , 1995, Nature.

[9]  Mark Ptashne,et al.  Negative effect of the transcriptional activator GAL4 , 1988, Nature.

[10]  R. Tjian,et al.  Drosophila TAFII150: similarity to yeast gene TSM-1 and specific binding to core promoter DNA. , 1994, Science.

[11]  B. Pugh,et al.  Dimerization of the TATA Binding Protein (*) , 1995, The Journal of Biological Chemistry.

[12]  R. Roeder,et al.  Cloning, functional characterization, and mechanism of action of the B-cell-specific transcriptional coactivator OCA-B , 1995, Molecular and cellular biology.

[13]  Masami Horikoshi,et al.  Transcription factor ATF interacts with the TATA factor to facilitate establishment of a preinitiation complex , 1988, Cell.

[14]  R. Tjian,et al.  Promoter-selective transcriptional defect in cell cycle mutant ts13 rescued by hTAFII250. , 1994, Science.

[15]  R. Tjian,et al.  Cloning and expression of human TAFII250: a TBP-associated factor implicated in cell-cycle regulation , 1993, Nature.

[16]  N. Gough.  Rapid and quantitative preparation of cytoplasmic RNA from small numbers of cells. , 1988, Analytical biochemistry.

[17]  J. Newell,et al.  OBF-1, a novel B cell-specific coactivator that stimulates immunoglobulin promoter activity through association with octamer-binding proteins , 1995, Cell.

[18]  R. Tjian,et al.  Molecular cloning and characterization of dTAFII30 alpha and dTAFII30 beta: two small subunits of Drosophila TFIID. , 1993, Genes & development.

[19]  Michael R. Green,et al.  Yeast TAF IIS in a multisubunit complex required for activated transcription , 1994, Nature.

[20]  R. Tjian,et al.  Drosophila tissue-specific transcription factor NTF-1 contains a novel isoleucine-rich activation motif. , 1993, Genes & development.

[21]  Y. Nakamura,et al.  The human CCG1 gene, essential for progression of the G1 phase, encodes a 210-kilodalton nuclear DNA-binding protein , 1991, Molecular and cellular biology.

[22]  K. Struhl,et al.  The glutamine-rich activation domains of human Sp1 do not stimulate transcription in Saccharomyces cerevisiae , 1995, Molecular and cellular biology.

[23]  R. Tjian,et al.  Drosophila TFIIA-L is processed into two subunits that are associated with the TBP/TAF complex. , 1993, Genes & development.

[24]  R. Tjian,et al.  Assembly of recombinant TFIID reveals differential coactivator requirements for distinct transcriptional activators , 1994, Cell.

[25]  M. Künzler,et al.  Functional differences between mammalian transcription activation domains at the yeast GAL1 promoter. , 1994, The EMBO journal.

[26]  R. Kornberg,et al.  Identification and characterization of a TFIID-like multiprotein complex from Saccharomyces cerevisiae. , 1995, Proceedings of the National Academy of Sciences of the United States of America.

[27]  R. Tjian,et al.  Coactivators for a proline-rich activator purified from the multisubunit human TFIID complex. , 1991, Genes & development.

[28]  Roger D. Kornberg,et al.  A novel mediator between activator proteins and the RNA polymerase II transcription apparatus , 1990, Cell.

[29]  B. Pugh,et al.  Dimerization of TFIID When Not Bound to DNA , 1996, Science.

[30]  R. Tjian,et al.  Drosophila TAFII40 interacts with both a VP16 activation domain and the basal transcription factor TFIIB , 1993, Cell.

[31]  R. Perry,et al.  The role of the κ enhancer and its binding factor NF-κB in the developmental regulation of κ gene transcription , 1987, Cell.

[32]  R. Tjian,et al.  Isolation of coactivators associated with the TATA-binding protein that mediate transcriptional activation , 1991, Cell.

[33]  Robert Tjian,et al.  Multiple TAFIIs Directing Synergistic Activation of Transcription , 1995, Science.

[34]  Tom Maniatis,et al.  Transcriptional activation: A complex puzzle with few easy pieces , 1994, Cell.

[35]  R. Tjian,et al.  Largest subunit of Drosophila transcription factor IID directs assembly of a complex containing TBP and a coactivator , 1993, Nature.

[36]  R. Tjian,et al.  TAFII250 Is a Bipartite Protein Kinase That Phosphorylates the Basal Transcription Factor RAP74 , 1996, Cell.

[37]  S. Berger,et al.  Selective inhibition of activated but not basal transcription by the acidic activation domain of VP16: Evidence for transcriptional adaptors , 1990, Cell.