A Simplified Method for Quick Detection of Rice Black-Streaked Dwarf Virus and Southern Rice Black-Streaked Dwarf Virus

Rice black-streaked dwarf virus and southern rice black-streaked dwarf virus are two important viruses in rice,which cause great losses in local rice production in recent years.The two viruses belong to Fijivirus.They share many similar characters,such as symptoms,virus particles,vectors,host range etc.,causing difficulty in discrimination of the two viruses.Three degenerate primers were designed and a simple,rapid,efficient method was described.