Chemical Constituents of the Essential Oils of White Turmeric (Curcuma zedoaria (Christm.) Roscoe) from Indonesia and its Toxicity toward Artemia salina Leach

Abstract White turmeric (Curcuma zedoaria (Christm.) Roscoe) has been used as traditional medicine by Indonesian people. The chemical constituents of essential oil of white turmeric were collected in 5 hours of the steam-distillation, and then were investigated by GC-MS. Furthermore, the essential oils were fractionated every hour of 5 hours distillation and were identified twenty compounds. The major compounds of the oil is camphor (49.51 %), isobornyl alcohol (12.66 %), borneol (4.23 %), furanodiene (3.61 %), furanodienone (3.49 %), 1,8-cineole (3.42 %), camphene (2.28 %), β-pinene (1.75 %), 2-nonanon (0.76 %) and germacrene- D (1.19 %). The main component of the essential oils is champhor, which is the highest concentration lie in the 3rd of collection times (50.71 %). The toxicity of the essential oils was subjected toward Artemia salina Leach. The results indicated that the essential oils from 5th of collection times by using steam-distillation have the highest toxicity, and the LC50 value is 3.25 ppm (v/v). The oil might be classified as anti-tumor activity.