Platelet Survival in Healthy Volunteers After Storage with N-(2-mercaptopropionyl)-glycine.

The sulphhydryl group containing drug N-(2-mercaptopropionyl)-glycine (MPG) was tested as a cytoprotective substance for storage of human platelets for transfusion. Autologous platelets stored for 48 h at 21 ± 1°C in the absence (control-platelets) or in the presence (MPG-platelets) of MPG, respectively, were labelled with Indium-III oxine and retransfused into the healthy donors. Significantly higher survival and a higher spleen/liver-ratio were obtained using stored MPG-platelets compared to stored control-platelets. Additionally, a very strong correlation was found between the percentage of discocytes counted after incubation of stored platelets in fresh plasma in vitro and the survival of stored platelets after retransfusion in vivo.

[1]  R. Aster,et al.  A Study of Variables Affecting the Quality of Platelets Stored at “Room Temperature” , 2003, Transfusion.

[2]  S. Moncada,et al.  Isolation and washing of human platelets with nitric oxide. , 1988, Thrombosis research.

[3]  Marek W. Radomski,et al.  An ultrastructural study of stored human platelets after washing using prostacyclin , 1985, British journal of haematology.

[4]  W. Armitage,et al.  Seleno-DL-methionine and the protection of human platelets against freezing injury. , 1985, Cryobiology.

[5]  S. Sasakawa,et al.  Changes of platelet membrane associated Ca2+ during storage and by 37C incubation with fresh plasma. , 1983, Thrombosis research.

[6]  H. Kotze,et al.  Quantification of in vivo distribution of platelets labeled with indium-111 oxine. , 1982, Journal of nuclear medicine : official publication, Society of Nuclear Medicine.

[7]  J. White,et al.  Influence of taxol on the response of platelets to chilling. , 1982, The American journal of pathology.

[8]  S. Slichter.  In vitro Measurements of Platelet Concentrates Stored at 4 and 22°C: Correlation with Posttransfusion Platelet Viability and Function , 1981, Vox sanguinis.

[9]  M. Mcgill.  Correlations of Platelet Survival and Function with the Results of Two in vitro Tests , 1981, Vox sanguinis.

[10]  J. White,et al.  Functional platelets after storage in vitro for 15--21 days. , 1980, The American journal of pathology.

[11]  T. Chiba,et al.  [Studies on thiol and disulfide compounds. I. Absorption distribution metabolism and excretion of 35 S-2-mercaptopropionylglycine]. , 1973, Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan.

[12]  F. Gardner,et al.  Platelet storage at 22 degrees C; metabolic, morphologic, and functional studies. , 1971, The Journal of clinical investigation.

[13]  U. Till,et al.  The role of the actin status in platelet behaviour. , 1988, Folia haematologica.

[14]  A. Ferrant.  Recommended Method for In-111 Platelet Survival Studies , 1988 .

[15]  G. Pescarmona,et al.  The use of N-(2-mercaptopropionyl)-glycine (MPG) for preparation and storage of platelets. , 1988, Folia haematologica.

[16]  S. Murphy.  Platelet Storage at 22°C , 1982 .

[17]  P. Badenhorst,et al.  Preparation of a Viable Population of Indium-111- Labelled Human Blood Platelets , 1979, Thrombosis and Haemostasis.