Analysis of cancer-related lncRNAs using gene ontology and KEGG pathways

BACKGROUND Cancer is a disease that involves abnormal cell growth and can invade or metastasize to other tissues. It is known that several factors are related to its initiation, proliferation, and invasiveness. Recently, it has been reported that long non-coding RNAs (lncRNAs) can participate in specific functional pathways and further regulate the biological function of cancer cells. Studies on lncRNAs are therefore helpful for uncovering the underlying mechanisms of cancer biological processes. METHODS We investigated cancer-related lncRNAs using gene ontology (GO) terms and KEGG pathway enrichment scores of neighboring genes that are co-expressed with the lncRNAs by extracting important GO terms and KEGG pathways that can help us identify cancer-related lncRNAs. The enrichment theory of GO terms and KEGG pathways was adopted to encode each lncRNA. Then, feature selection methods were employed to analyze these features and obtain the key GO terms and KEGG pathways. RESULTS The analysis indicated that the extracted GO terms and KEGG pathways are closely related to several cancer associated processes, such as hormone associated pathways, energy associated pathways, and ribosome associated pathways. And they can accurately predict cancer-related lncRNAs. CONCLUSIONS This study provided novel insight of how lncRNAs may affect tumorigenesis and which pathways may play important roles during it. These results could help understanding the biological mechanisms of lncRNAs and treating cancer.

[1]  Xiaojing Wang,et al.  Proteogenomic analysis reveals unanticipated adaptations of colorectal tumor cells to deficiencies in DNA mismatch repair. , 2014, Cancer research.

[2]  K. Hua,et al.  Overexpression of long non-coding RNA HOTAIR predicts poor patient prognosis and promotes tumor metastasis in epithelial ovarian cancer. , 2014, Gynecologic oncology.

[3]  Yongzhan Nie,et al.  Long Noncoding RNA MRUL Promotes ABCB1 Expression in Multidrug-Resistant Gastric Cancer Cell Sublines , 2014, Molecular and Cellular Biology.

[4]  Shuangjie Wu,et al.  Original Article LncRNA expression profiles reveal the co-expression network in human colorectal carcinoma , 2016 .

[5]  Weidong Huang,et al.  Genome-wide cloning, bioinformatic analysis and expression pattern of phospholipase D gene family in Vitis vinifera , 2016 .

[6]  Hassan Mohabatkar,et al.  Prediction of allergenic proteins by means of the concept of Chou's pseudo amino acid composition and a machine learning approach. , 2012, Medicinal chemistry (Shariqah (United Arab Emirates)).

[7]  Kenji Sakimura,et al.  NG2 proteoglycan-dependent recruitment of tumor macrophages promotes pericyte-endothelial cell interactions required for brain tumor vascularization , 2015, Oncoimmunology.

[8]  Hermann-Georg Holzhütter,et al.  Physiology-Based Kinetic Modeling of Neuronal Energy Metabolism Unravels the Molecular Basis of NAD(P)H Fluorescence Transients , 2015, Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism.

[9]  S. Sathiya Keerthi,et al.  Improvements to Platt's SMO Algorithm for SVM Classifier Design , 2001, Neural Computation.

[10]  Lennart Martens,et al.  LNCipedia: a database for annotated human lncRNA transcript sequences and structures , 2012, Nucleic Acids Res..

[11]  Xing Chen,et al.  LncRNADisease: a database for long-non-coding RNA-associated diseases , 2012, Nucleic Acids Res..

[12]  Suresh Ramakrishna,et al.  Critical lysine residues of Klf4 required for protein stabilization and degradation. , 2014, Biochemical and biophysical research communications.

[13]  Ralph Scully,et al.  BRCA1 controls homologous recombination at Tus/Ter-stalled mammalian replication forks , 2014, Nature.

[14]  D. Erie,et al.  Single molecule studies of DNA mismatch repair. , 2014, DNA repair.

[15]  Li Li,et al.  Oncogenes associated with drug resistance in ovarian cancer , 2015, Journal of Cancer Research and Clinical Oncology.

[16]  C. Feschotte,et al.  Volatile evolution of long noncoding RNA repertoires: mechanisms and biological implications. , 2014, Trends in genetics : TIG.

[17]  Kotb Abdelmohsen,et al.  Posttranscriptional gene regulation by long noncoding RNA. , 2013, Journal of molecular biology.

[18]  A. Kong,et al.  Dietary phytochemicals and cancer prevention: Nrf2 signaling, epigenetics, and cell death mechanisms in blocking cancer initiation and progression. , 2013, Pharmacology & therapeutics.

[19]  Bo Hu,et al.  Noncoding RNAs in cancer and cancer stem cells , 2013, Chinese journal of cancer.

[20]  Alberto Passi,et al.  Natural Antisense Transcript for Hyaluronan Synthase 2 (HAS2-AS1) Induces Transcription of HAS2 via Protein O-GlcNAcylation* , 2014, The Journal of Biological Chemistry.

[21]  Michael R Green,et al.  The CREB Coactivator CRTC2 Is a Lymphoma Tumor Suppressor that Preserves Genome Integrity through Transcription of DNA Mismatch Repair Genes. , 2015, Cell reports.

[22]  Tushar Patel,et al.  Involvement of Extracellular Vesicle Long Noncoding RNA (linc-VLDLR) in Tumor Cell Responses to Chemotherapy , 2014, Molecular Cancer Research.

[23]  Q. Guo,et al.  Comprehensive analysis of lncRNA-mRNA co-expression patterns identifies immune-associated lncRNA biomarkers in ovarian cancer malignant progression , 2015, Scientific Reports.

[24]  K. Chou,et al.  Predicting Anatomical Therapeutic Chemical (ATC) Classification of Drugs by Integrating Chemical-Chemical Interactions and Similarities , 2012, PloS one.

[25]  R. Trumbly,et al.  Differential effects of estrogen-dependent transactivation vs. transrepression by the estrogen receptor on invasiveness of HER2 overexpressing breast cancer cells. , 2015, Biochemical and biophysical research communications.

[26]  A. Wierzbicki,et al.  A SWI/SNF Chromatin-Remodeling Complex Acts in Noncoding RNA-Mediated Transcriptional Silencing , 2013, Molecular cell.

[27]  Nicolas Binder,et al.  Tumor and Patient Characteristics of Individuals with Mismatch Repair Deficient Colorectal Cancer , 2015, Digestion.

[28]  Shinichi Nakagawa,et al.  The long noncoding RNA Neat1 is required for mammary gland development and lactation , 2014, RNA.

[29]  Limei Ma,et al.  C-Myc-activated long noncoding RNA CCAT1 promotes colon cancer cell proliferation and invasion , 2014, Tumor Biology.

[30]  BiaoYan,et al.  lncRNA-MIAT Regulates Microvascular Dysfunction by Functioning as a Competing Endogenous RNA , 2015 .

[31]  Tokuji Tsuchiya,et al.  Genomic structure and differential expression of two tandem-arranged GSTZ genes in rice. , 2004, Gene.

[32]  Yunshan Wang,et al.  The Hedgehog pathway: role in cell differentiation, polarity and proliferation , 2015, Archives of Toxicology.

[33]  Jing Chen,et al.  Lysine acetylation activates 6-phosphogluconate dehydrogenase to promote tumor growth. , 2014, Molecular cell.

[34]  S. Weissman,et al.  Long intergenic non-coding RNA HOTAIRM1 regulates cell cycle progression during myeloid maturation in NB4 human promyelocytic leukemia cells , 2014, RNA biology.

[35]  Lei Chen,et al.  Analysis of Tumor Suppressor Genes Based on Gene Ontology and the KEGG Pathway , 2014, PloS one.

[36]  Jia Yu,et al.  PU.1-Regulated Long Noncoding RNA lnc-MC Controls Human Monocyte/Macrophage Differentiation through Interaction with MicroRNA 199a-5p , 2015, Molecular and Cellular Biology.

[37]  Jun Liu,et al.  LncRNA expression profiles reveal the co-expression network in human colorectal carcinoma , 2016 .

[38]  Ian Witten,et al.  Data Mining , 2000 .

[39]  André Toulouse,et al.  Allelic methylation bias of the RARB2 tumor suppressor gene promoter in cancer , 2008, Genes, chromosomes & cancer.

[40]  Ebrahim Hajizadeh,et al.  Finding genes discriminating smokers from non-smokers by applying a growing self-organizing clustering method to large airway epithelium cell microarray data. , 2013, Asian Pacific journal of cancer prevention : APJCP.

[41]  Meng Li,et al.  Silencing of Long Noncoding RNA MALAT1 by miR-101 and miR-217 Inhibits Proliferation, Migration, and Invasion of Esophageal Squamous Cell Carcinoma Cells* , 2014, The Journal of Biological Chemistry.

[42]  Susan Carpenter,et al.  Transcription of inflammatory genes: long noncoding RNA and beyond. , 2015, Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research.

[43]  T. Kensler,et al.  Nrf2: control of sensitivity to carcinogens , 2011, Archives of Toxicology.

[44]  C J Kemp,et al.  p27(Kip1): regulation and function of a haploinsufficient tumor suppressor and its misregulation in cancer. , 2001, Experimental cell research.

[45]  J. Higginson,et al.  International Agency for Research on Cancer. , 1968, WHO chronicle.

[46]  Marta Alegre,et al.  Locally advanced and metastatic basal cell carcinoma: molecular pathways, treatment options and new targeted therapies , 2014, Expert review of anticancer therapy.

[47]  Toshifumi Inada,et al.  Stress‐induced lncRNAs evade nuclear degradation and enter the translational machinery , 2013, Genes to cells : devoted to molecular & cellular mechanisms.

[48]  Thanos D Halazonetis,et al.  DNA replication stress as a hallmark of cancer. , 2015, Annual review of pathology.

[49]  T. Wang,et al.  Long noncoding RNA PEG10 regulates proliferation and invasion of esophageal cancer cells , 2015, Cancer Gene Therapy.

[50]  R. Young,et al.  Transcriptional Regulation and Its Misregulation in Disease , 2013, Cell.

[51]  B. Benayoun,et al.  Adult ovarian granulosa cell tumor transcriptomics: prevalence of FOXL2 target genes misregulation gives insights into the pathogenic mechanism of the p.Cys134Trp somatic mutation , 2013, Oncogene.

[52]  L. Boyer,et al.  Lncing epigenetic control of transcription to cardiovascular development and disease. , 2015, Circulation research.

[53]  อนิรุธ สืบสิงห์,et al.  Data Mining Practical Machine Learning Tools and Techniques , 2014 .

[54]  Hagai Bergman,et al.  Long Non-Coding RNA and Alternative Splicing Modulations in Parkinson's Leukocytes Identified by RNA Sequencing , 2014, PLoS Comput. Biol..

[55]  Guodong Yang,et al.  LncRNA: a link between RNA and cancer. , 2014, Biochimica et biophysica acta.

[56]  Pierre Baldi,et al.  Assessing the accuracy of prediction algorithms for classification: an overview , 2000, Bioinform..

[57]  Xiao Yang,et al.  Long non-coding RNA HNF1A-AS1 regulates proliferation and migration in oesophageal adenocarcinoma cells , 2013, Gut.

[58]  Tao Huang,et al.  Identification of compound–protein interactions through the analysis of gene ontology, KEGG enrichment for proteins and molecular fragments of compounds , 2016, Molecular Genetics and Genomics.

[59]  T. Chan,et al.  Therapeutic targeting of tumor suppressor genes , 2015, Cancer.

[60]  James T. Elder,et al.  Analysis of long non-coding RNAs highlights tissue-specific expression patterns and epigenetic profiles in normal and psoriatic skin , 2015, Genome Biology.

[61]  M. Roizen,et al.  Hallmarks of Cancer: The Next Generation , 2012 .

[62]  Robert L Sutherland,et al.  ZNF300P1 Encodes a lincRNA that regulates cell polarity and is epigenetically silenced in type II epithelial ovarian cancer , 2014, Molecular Cancer.

[63]  Xin Sheng Wang,et al.  Warburg Effect or Reverse Warburg Effect? A Review of Cancer Metabolism , 2015, Oncology Research and Treatment.

[64]  N. Sonenberg,et al.  Targeting the translation machinery in cancer , 2015, Nature Reviews Drug Discovery.

[65]  Lei Chen,et al.  Prediction of Metabolic Pathway Using Graph Property, Chemical Functional Group and Chemical Structural Set , 2013 .

[66]  Frank Gibbons,et al.  Lifespan Based Pharmacokinetic-Pharmacodynamic Model of Tumor Growth Inhibition by Anticancer Therapeutics , 2014, PloS one.

[67]  Majid Mohammad Beigi,et al.  Prediction of allergenic proteins by means of the concept of Chou's pseudo amino acid composition and a machine learning approach. , 2012 .

[68]  Brian T. Lee,et al.  The UCSC Genome Browser database: 2015 update , 2014, Nucleic Acids Res..

[69]  Susumu Goto,et al.  Data, information, knowledge and principle: back to metabolism in KEGG , 2013, Nucleic Acids Res..

[70]  Emily A. Vucic,et al.  Human Cancer Long Non-Coding RNA Transcriptomes , 2011, PloS one.

[71]  Rob Jelier,et al.  A Long Noncoding RNA on the Ribosome Is Required for Lifespan Extension. , 2015, Cell reports.

[72]  Jun-Qi Ma,et al.  Epigenetic regulation of human riboflavin transporter 2(hRFT2) in cervical cancers from Uighur women. , 2014, Asian Pacific journal of cancer prevention : APJCP.

[73]  Steven Y. Cheng,et al.  MicroRNA-31 suppresses medulloblastoma cell growth by inhibiting DNA replication through minichromosome maintenance 2 , 2014, Oncotarget.

[74]  Hu Pu,et al.  LncRNA HOTAIR promotes human liver cancer stem cell malignant growth through downregulation of SETD2 , 2015, Oncotarget.

[75]  Min Yu,et al.  Targeting homologous recombination and telomerase in Barrett’s adenocarcinoma: Impact on telomere maintenance, genomic instability, and tumor growth , 2013, Oncogene.

[76]  Jing Bai,et al.  Identification of cancer-related lncRNAs through integrating genome, regulome and transcriptome features. , 2015, Molecular bioSystems.

[77]  Alberto Passi,et al.  The Motile Breast Cancer Phenotype Roles of Proteoglycans/Glycosaminoglycans , 2014, BioMed research international.

[78]  Ian H. Witten,et al.  Data mining: practical machine learning tools and techniques, 3rd Edition , 1999 .

[79]  David Haussler,et al.  Current status and new features of the Consensus Coding Sequence database , 2013, Nucleic Acids Res..

[80]  Sylvain Julien,et al.  Over-sulfated glycosaminoglycans are alternative selectin ligands: insights into molecular interactions and possible role in breast cancer metastasis , 2013, Clinical & Experimental Metastasis.

[81]  R. Hernández-Muñoz,et al.  α-Tocopherol administration blocks adaptive changes in cell NADH/NAD+ redox state and mitochondrial function leading to inhibition of gastric mucosa cell proliferation in rats. , 2013, Free radical biology & medicine.

[82]  S. Sokol,et al.  Wnt signaling and dorso-ventral axis specification in vertebrates. , 1999, Current opinion in genetics & development.

[83]  Steven J. M. Jones,et al.  Subgroup-specific structural variation across 1,000 medulloblastoma genomes , 2012, Nature.

[84]  Juancarlos Chan,et al.  Gene Ontology Consortium: going forward , 2014, Nucleic Acids Res..

[85]  Wei Li,et al.  Enzymatic synthesis of glutathione using yeast cells in two-stage reaction , 2010, Bioprocess and biosystems engineering.

[86]  M. Mourtada-Maarabouni,et al.  Long non-coding RNA GAS5 regulates apoptosis in prostate cancer cell lines. , 2013, Biochimica et biophysica acta.

[87]  Luísa Azevedo,et al.  NAMPT and NAPRT1: novel polymorphisms and distribution of variants between normal tissues and tumor samples , 2014, Scientific Reports.

[88]  Qiuhui Pan,et al.  Mutual inhibition between YAP and SRSF1 maintains long non-coding RNA, Malat1-induced tumourigenesis in liver cancer. , 2014, Cellular signalling.

[89]  Ron Kohavi,et al.  A Study of Cross-Validation and Bootstrap for Accuracy Estimation and Model Selection , 1995, IJCAI.

[90]  G. H. Clowes,et al.  Glucose, Mannose, and Fructose Metabolism by Ascites Tumor Cells , 1954, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[91]  Paolo Vineis,et al.  DNA methylation changes associated with cancer risk factors and blood levels of vitamin metabolites in a prospective study , 2011, Epigenetics.

[92]  Tadashi Eguchi,et al.  Biosynthetic enzymes for the aminoglycosides butirosin and neomycin. , 2009, Methods in enzymology.

[93]  Mohammad Athar,et al.  Role of CRD-BP in the growth of human Basal Cell Carcinoma Cells , 2014, The Journal of investigative dermatology.

[94]  Fuhui Long,et al.  Feature selection based on mutual information criteria of max-dependency, max-relevance, and min-redundancy , 2003, IEEE Transactions on Pattern Analysis and Machine Intelligence.

[95]  Larisa M Haupt,et al.  Heparan Sulfate Proteoglycans and Human Breast Cancer Epithelial Cell Tumorigenicity , 2014, Journal of cellular biochemistry.

[96]  Fang Wang,et al.  Oncofetal long noncoding RNA PVT1 promotes proliferation and stem cell‐like property of hepatocellular carcinoma cells by stabilizing NOP2 , 2014, Hepatology.

[97]  中尾 光輝,et al.  KEGG(Kyoto Encyclopedia of Genes and Genomes)〔和文〕 (特集 ゲノム医学の現在と未来--基礎と臨床) -- (データベース) , 2000 .

[98]  I. Kurochkin,et al.  Long noncoding RNAs: a potential novel class of cancer biomarkers , 2015, Front. Genet..

[99]  John C. Platt,et al.  Fast training of support vector machines using sequential minimal optimization, advances in kernel methods , 1999 .

[100]  Chen Chu,et al.  Predicting the types of metabolic pathway of compounds using molecular fragments and sequential minimal optimization. , 2016, Combinatorial chemistry & high throughput screening.

[101]  P. Mehdipour,et al.  Association of folate and other one-carbon related nutrients with hypermethylation status and expression of RARB, BRCA1, and RASSF1A genes in breast cancer patients , 2015, Journal of Molecular Medicine.

[102]  Bruce Stillman,et al.  DDX5 regulates DNA replication and is required for cell proliferation in a subset of breast cancer cells. , 2012, Cancer discovery.

[103]  Ding Ma,et al.  Sine oculis homeobox homolog 1 promotes DNA replication and cell proliferation in cervical cancer. , 2014, International journal of oncology.

[104]  Hui Chen,et al.  Aberrant Expression miR-196a Is Associated With Abnormal Apoptosis, Invasion, and Proliferation of Pancreatic Cancer Cells , 2013, Pancreas.

[105]  Zhihua Liu,et al.  CGAP: a new comprehensive platform for the comparative analysis of chloroplast genomes , 2013, BMC Bioinformatics.

[106]  M Fickova,et al.  Impact of endocrine disruptors on ovarian steroidogenesis. , 2014, Endocrine regulations.

[107]  Yanli Wang,et al.  FSelector: a Ruby gem for feature selection , 2012, Bioinform..

[108]  Zhiguo Zhang,et al.  Increased New lncRNA–mRNA Gene Pair Levels in Human Cumulus Cells Correlate With Oocyte Maturation and Embryo Development , 2015, Reproductive Sciences.

[109]  A. Bhan,et al.  Antisense transcript long noncoding RNA (lncRNA) HOTAIR is transcriptionally induced by estradiol. , 2013, Journal of molecular biology.

[110]  Paolo Vineis,et al.  Towards incorporating epigenetic mechanisms into carcinogen identification and evaluation. , 2013, Carcinogenesis.

[111]  Udo Schumacher,et al.  Aberrant Presentation of HPA-Reactive Carbohydrates Implies Selectin-Independent Metastasis Formation in Human Prostate Cancer , 2014, Clinical Cancer Research.

[112]  Lei Chen,et al.  Identification of Drug-Drug Interactions Using Chemical Interactions , 2017 .

[113]  Jiri Bartek,et al.  Myc and Ras oncogenes engage different energy metabolism programs and evoke distinct patterns of oxidative and DNA replication stress , 2014, Molecular oncology.

[114]  C. la Vecchia,et al.  Lactose absorption in patients with ovarian cancer. , 1999, American journal of epidemiology.

[115]  B. Matthews.  Comparison of the predicted and observed secondary structure of T4 phage lysozyme. , 1975, Biochimica et biophysica acta.

[116]  Basappa,et al.  Roles of glycosaminoglycans and glycanmimetics in tumor progression and metastasis , 2014, Glycoconjugate Journal.

[117]  Li Wang,et al.  Long non-coding RNA HOTAIR in carcinogenesis and metastasis. , 2014, Acta biochimica et biophysica Sinica.

[118]  Chen Chu,et al.  Prediction and analysis of cell-penetrating peptides using pseudo-amino acid composition and random forest models , 2015, Amino Acids.

[119]  J. O'Callaghan,et al.  Biomarkers of Parkinson's disease: present and future. , 2015, Metabolism: clinical and experimental.

[120]  Guoxin Zhang,et al.  The Interaction Between MiR-141 and lncRNA-H19 in Regulating Cell Proliferation and Migration in Gastric Cancer , 2015, Cellular Physiology and Biochemistry.

[121]  Tao Huang,et al.  Genetic differences among ethnic groups , 2015, BMC Genomics.

[122]  Lior Pachter,et al.  Sequence Analysis , 2020, Definitions.

[123]  A. Amedei,et al.  The use of cytokines and chemokines in the cancer immunotherapy. , 2013, Recent patents on anti-cancer drug discovery.

[124]  Kuo-Chen Chou,et al.  Predicting the network of substrate-enzyme-product triads by combining compound similarity and functional domain composition , 2010, BMC Bioinformatics.

[125]  David R. Kelley,et al.  Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks , 2012, Nature Protocols.

[126]  T. Jaffredo,et al.  Cell interactions and cell signaling during hematopoietic development. , 2014, Experimental cell research.

[127]  F. Pauler,et al.  Long non-coding RNAs display higher natural expression variation than protein-coding genes in healthy humans , 2016, Genome Biology.

[128]  Zhibo Hou,et al.  A long noncoding RNA Sox2ot regulates lung cancer cell proliferation and is a prognostic indicator of poor survival. , 2014, The international journal of biochemistry & cell biology.

[129]  Xuan Zhou,et al.  Classification of G-protein coupled receptors based on support vector machine with maximum relevance minimum redundancy and genetic algorithm , 2010, BMC Bioinformatics.

[130]  Kazuyuki Sugahara,et al.  Receptor for Advanced Glycation End Products (RAGE) Functions as Receptor for Specific Sulfated Glycosaminoglycans, and Anti-RAGE Antibody or Sulfated Glycosaminoglycans Delivered in Vivo Inhibit Pulmonary Metastasis of Tumor Cells* , 2012, The Journal of Biological Chemistry.

[131]  Yujin Hoshida,et al.  DNA hypomethylation induces a DNA replication-associated cell cycle arrest to block hepatic outgrowth in uhrf1 mutant zebrafish embryos , 2015 .

[132]  B. Luisi,et al.  Rarely at rest , 2013, RNA biology.

[133]  Wei Chen,et al.  Urothelial carcinoma associated 1 is a hypoxia-inducible factor-1α-targeted long noncoding RNA that enhances hypoxic bladder cancer cell proliferation, migration, and invasion , 2014, Tumor Biology.

[134]  Lanfen Chen,et al.  The Hippo signaling pathway in liver regeneration and tumorigenesis. , 2015, Acta biochimica et biophysica Sinica.

[135]  Ian H. Witten,et al.  Stacking Bagged and Dagged Models , 1997, ICML.

[136]  Chris Sander,et al.  Comprehensive Analysis of Long Non-Coding RNAs in Ovarian Cancer Reveals Global Patterns and Targeted DNA Amplification , 2013, PloS one.

[137]  C. Plass,et al.  Loss of imprinting of the insulin-like growth factor II gene occurs by biallelic methylation in a core region of H19-associated CTCF-binding sites in colorectal cancer. , 2001, Proceedings of the National Academy of Sciences of the United States of America.

[138]  J. Woolford,et al.  Ribosome Biogenesis in the Yeast Saccharomyces cerevisiae , 2013, Genetics.

[139]  I. Gérin,et al.  Multiple Roles for the Non-Coding RNA SRA in Regulation of Adipogenesis and Insulin Sensitivity , 2010, PloS one.

[140]  J. Wilusz.  Long noncoding RNAs: Re-writing dogmas of RNA processing and stability. , 2016, Biochimica et biophysica acta.