High resolution separation methods for the determination of intact human erythropoiesis stimulating agents. A review.

Dopamine D3 receptors (Sokoloff et al., 1990) have been shown to be related to dopamine D2 receptors and have been suggested to play a role in mediating the antipsychotic effects of neuroleptics. So far studies on the expression of D3 mRNA and of binding sites with pharmacological characteristics of D3 receptors have been restricted to rat brain. Using in situ hybridization histochemistry, we demonstrate that D3 mRNAs are enriched in human n, accumbens and in the islands of Calleja. In addition, D3 mRNA was detected at very low levels in anterior caudate and putamen with a rostro-caudally decreasing gradient and in hypothalamic mammillary nuclei. In receptor autoradiographic binding studies, the islands of Calleja were found to be labeled by [125I]iodosulpride and [3H]CV 205 502 but not by [3H]raclopride and [3H]YM 09151-2. Pharmacological analysis of binding of the D2/D3 ligand [3H]CV 205 502 in n. accumbens and caudate-putamen is consistent with the presence of D3 receptor sites in ventral striatum. Overall distribution and pharmacology of D3 sites in human and rat brain appear to be similar. Presence and distribution of D3 receptors in human brain are compatible with the notion that D3 receptors might be involved in mediating the clinical effects of antipsychotics.

[1]  J. Palacios,et al.  Visualization of a dopamine D1 receptor mRNA in human and rat brain. , 1991, Brain research. Molecular brain research.

[2]  M. Martres,et al.  Alternative splicing directs the expression of two D2 dopamine receptor isoforms , 1989, Nature.

[3]  J. Palacios,et al.  Visualization of dopamine D1, D2 and D3 receptor mRNA's in human and rat brain , 1992, Neurochemistry International.

[4]  K. Fuxe,et al.  EVIDENCE FOR THE EXISTENCE OF MONOAMINE-CONTAINING NEURONS IN THE CENTRAL NERVOUS SYSTEM. I. DEMONSTRATION OF MONOAMINES IN THE CELL BODIES OF BRAIN STEM NEURONS. , 1964, Acta physiologica Scandinavica. Supplementum.

[5]  M. Martres,et al.  Pharmacology of human dopamine D3 receptor expressed in a mammalian cell line: comparison with D2 receptor. , 1992, European journal of pharmacology.

[6]  J. Palacios,et al.  Localization of the mRNA for the dopamine D2 receptor in the rat brain by in situ hybridization histochemistry. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[7]  D. Grandy,et al.  Cloning and expression of a rat D2 dopamine receptor cDNA , 1988, Nature.

[8]  J. Palacios,et al.  Differential Visualization of Dopamine D2 and D3 Receptor Sites in Rat Brain. A Comparative Study Using In Situ Hybridization Histochemistry and Ligand Binding Autoradiography , 1993, The European journal of neuroscience.

[9]  D. Grandy,et al.  Cloning and expression of human and rat Dt dopamine receptors , 1990, Nature.

[10]  M. Martres,et al.  The third dopamine receptor (D3) as a novel target for antipsychotics. , 1992, Biochemical pharmacology.

[11]  J. Palacios,et al.  Dopamine D2 receptors in the rat brain: autoradiographic visualization using a high-affinity selective agonist ligand , 1987, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[12]  M. Martres,et al.  Localization and function of the D3 dopamine receptor. , 1992, Arzneimittel-Forschung.

[13]  C. Gerfen,et al.  Molecular cloning and expression of a D1 dopamine receptor linked to adenylyl cyclase activation. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[14]  D. Grandy,et al.  Cloning of the cDNA and gene for a human D2 dopamine receptor. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[15]  R. Rozmahel,et al.  Human dopamine D1 receptor encoded by an intronless gene on chromosome 5 , 1990, Nature.

[16]  M. Martres,et al.  Widespread distribution of brain dopamine receptors evidenced with [125I]iodosulpride, a highly selective ligand. , 1985, Science.

[17]  C J CLEMEDSON,et al.  DYNAMIC RESPONSE OF CHEST WALL AND LUNG INJURIES IN RABBITS EXPOSED TO AIR SHOCK WAVES OF SHORT DURATION. , 1964, Acta physiologica Scandinavica. Supplementum.

[18]  M. Martres,et al.  A detailed mapping of dopamine D-2 receptors in rat central nervous system by autoradiography with [125I]iodosulpride , 1987, Neuroscience.

[19]  Susan R. George,et al.  Cloning of the gene for a human dopamine D5 receptor with higher affinity for dopamine than D1 , 1991, Nature.

[20]  Philip Seeman,et al.  Cloning of the gene for a human dopamine D4 receptor with high affinity for the antipsychotic clozapine , 1991, Nature.

[21]  M. Terai,et al.  Comparison of [3H]YM-09151-2 with [3H]spiperone and [3H]raclopride for dopamine d-2 receptor binding to rat striatum. , 1989, European journal of pharmacology.

[22]  A. Unis,et al.  Brain receptor autoradiography with [3H]-YM 09151-2: a ligand for labeling dopamine D-2 receptors. , 1990, Life sciences.

[23]  M. Martres,et al.  [Gene cloning of human dopaminergic D3 receptor and identification of its chromosome]. , 1990, Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie.

[24]  C. Gerfen,et al.  Multiple D2 dopamine receptors produced by alternative RNA splicing , 1989, Nature.

[25]  M. Caron,et al.  Molecular cloning and expression of the gene for a human D1 dopamine receptor , 1990, Nature.

[26]  Bruno Giros,et al.  Localization of dopamine D3 receptor mRNA in the rat brain using in situ hybridization histochemistry: comparison with dopamine D2 receptor mRNA , 1991, Brain Research.

[27]  Bruno Giros,et al.  Molecular cloning and characterization of a novel dopamine receptor (D3) as a target for neuroleptics , 1990, Nature.

[28]  F. J. White,et al.  Differential effects of classical and atypical antipsychotic drugs on A9 and A10 dopamine neurons. , 1983, Science.