Pore-size controlled and polycation-immobilized cellulose spherical particles for removal of endotoxin

セルロース微粒子を基材とし，これに種々のポリカチオンを化学修飾した高分子球状微粒子を調製した．得られた種々のポリカチオン固定化セルロース粒子をエンドトキシン(LPS)吸着剤として用い，バッチ法により LPS 水溶液からの LPS 吸着除去を試みた．その結果，すべての粒子の LPS 吸着能は，粒子の細孔径(糖の排除限界分子；Mlim)の増大とともに著しく増大し，吸着時の緩衝液のイオン強度の上昇とともに低下した．種々の吸着剤のタンパク質溶液からの LPS 選択除去能をカラム法により評価した結果，ポリ(e-リジン)固定化セルロース粒子は pH 7，イオン強度 μ=0.17 の条件下で，アルブミンを吸着することなく，LPS を吸着除去できた．吸着処理後の溶液中の LPS 残存濃度は 10 pg/mL 以下で，タンパク質回収率は 98%であった．これらの結果から，同粒子はタンパク質水溶液からの LPS 吸着除去剤として大いに期待できることがわかった．

[1]  M. Sakata,et al.  Chromatographic removal of endotoxin from protein solutions by polymer particles. , 2002, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[2]  M. Kunitake,et al.  Pore-size controlled and poly(ε-lysine)-immobilized cellulose spherical particles for removal of lipopolysaccharides , 2002 .

[3]  F B Anspach,et al.  Endotoxin removal from protein solutions. , 2000, Journal of Biotechnology.

[4]  H. Ihara,et al.  Reduction of endotoxin contamination of various crude vaccine materials by gram-negative bacteria using aminated poly(gamma-methyl L-glutamate) spherical particles. , 1993, Biological & pharmaceutical bulletin.

[5]  M. Kastowsky,et al.  Phase behavior, supramolecular structure, and molecular conformation of lipopolysaccharide. , 1993, Immunobiology.

[6]  H. Ihara,et al.  Effect of the Pore Size of an A initiated Poly(γ-methyl l-glutamate) Adsorbent on the Selective Removal of Endotoxin , 1992 .

[7]  H. Ihara,et al.  Regulation of Pore-size Distribution of Poly(γ-methyl L-glutamate) Spheres as a Gel Permeation Chromatography Packings , 1990 .

[8]  T. Watanabe,et al.  Characteristics and applications of adsorbents for pyrogen removal , 1988, Biotechnology and applied biochemistry.

[9]  H. Ihara,et al.  Preparation and gel permeation chromatographic properties of porous spheres from poly (γ-methyl or γ-benzyl L-glutamate) , 1986 .

[10]  H. Ihara,et al.  Novel gel permeation chromatography packing composed of cross-linked and porous spherical particles of poly-γ-methyl-l-glutamate , 1985 .

[11]  A. Issekutz.  Removal of gram-negative endotoxin from solutions by affinity chromatography. , 1983, Journal of immunological methods.

[12]  T. Tosa,et al.  Preparation of adsorbents for pyrogen adsorption , 1982 .

[13]  S. Kuga.  The porous structure of cellulose gel regenerated from calcium thiocyanate solution , 1980 .

[14]  H. Sakai,et al.  Polylysine Produced by Streptomyces , 1977 .