Flow cytometric analysis of immunogold cell surface label.

Goat antimouse immunoglobulin antibodies conjugated to colloidal 40-nm gold particles were used to label mouse spleen lymphocytes. The labeled cells were analysed with a flow cytometer, equipped with an argon-ion laser and a (0.5 mW) helium-neon laser. The right-angle (90 degrees) light scatter signal of the red (632.8 nm) helium-neon light was enhanced more than tenfold by the gold label. Dual labeling with gold and fluorescein isothiocyanate (FITC) showed no interference between the two labels. Thus immunogold provides a nonfluorescent cell surface label that can be combined with other cell labels for multiparametrical cell analysis.

[1]  H M Shapiro,et al.  Multistation multiparameter flow cytometry: a critical review and rationale. , 1983, Cytometry.

[2]  M. Loken.  Simultaneous quantitation of Hoechst 33342 and immunofluorescence on viable cells using a fluorescence activated cell sorter. , 1980, Cytometry.

[3]  M. De Brabander,et al.  High resolution light and electron microscopic localization of tubulin with the IGS (immuno gold staining) method. , 1981, Cell biology international reports.

[4]  W. D. Geoghegan,et al.  Adsorption of horseradish peroxidase, ovomucoid and anti-immunoglobulin to colloidal gold for the indirect detection of concanavalin A, wheat germ agglutinin and goat anti-human immunoglobulin G on cell surfaces at the electron microscopic level: a new method, theory and application. , 1977, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[5]  J. Steinkamp,et al.  Three-color fluorescence measurements on single cells excited at three laser wavelengths. , 2005, Cytometry.

[6]  I. W. Mclean,et al.  PERIODATE-LYSINE-PARAFORMALDEHYDE FIXATIVE A NEW FIXATIVE FOR IMMUNOELECTRON MICROSCOPY , 1974, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[7]  Y. Konttinen,et al.  A method for the identification of human peripheral blood T lymphocytes by sequential immunogold and esterase double staining. , 1983, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[8]  M. Horisberger,et al.  Colloidal gold : a cytochemical marker for light and fluorescent microscopy and for transmission and scanning electron microscopy. , 1981, Scanning electron microscopy.