In this paper, the rule of the generation of single-machine operation skill is studied through experiment, which can make the operator adapt to operating environment quickly and complete the task. Methods: Multi-group operation was carried out to explore the basic rules of skills by recording experimental data, drawing curve dynamic graph, and comparing multiple sets of data. Through the combination of theory and practice, the general rule of skill generation was obtained by analyzing the graph, and there was a certain mechanism. Results: Whether single-person or multi-person operation, its skill generation rules follow a certain pattern, but complex operations and multi-person operations are more complex.