Studies of Fe3O4-chitosan nanoparticles prepared by co-precipitation under the magnetic field for lipase immobilization

Abstract A simple method was introduced to prepare magnetic Fe3O4-chitosan nanoparticles by co-precipitation with 0.45 T static magnetic field via glutaraldehyde cross-linking reaction, and the nanoparticles were used to immobilize lipase. The influence of magnetic field on the properties of magnetic nanoparticles was studied by SEM, XRD and VSM. The results showed that no obvious difference of the nanoparticles structure was found, while the morphology varied from random sphere-like to rod-like and magnetic character was significantly changed. Furthermore, the lipase immobilized with nanoparticles prepared under the magnetic field enhanced the activity, operational and storage stability compared with the control sample.

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