Photosynthetic Light Reactions in Chemically Fixed Anacystis nidulans, Chlorella pyrenoidosa, and Porphyridium cruentum.

The photochemical activities of various species of unicellular algae (Anacystis nidulans, Chlorella pyrenoidosa, and Porphyridium cruentum) were studied following chemical fixation. Fixation with formaldehyde and glutaraldehyde yielded cells which retained their ability to perform photosystem I and photosystem II reactions. The photochemical efficiencies of some fixed algae are as great as those of unfixed spinach chloroplasts. Fixed algae containing accessory pigments appear to be useful models for further studies of the light reactions of photosynthesis.

[1]  P. J. Anderson.  PURIFICATION AND QUANTITATION OF GLUTARALDEHYDE AND ITS EFFECT ON SEVERAL ENZYME ACTIVITIES IN SKELETAL MUSCLE , 1967, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[2]  J. Biggins,et al.  Photosynthetic Reactions by Lysed Protoplasts and Particle Preparations from the Blue-Green Alga, Phormidium luridum. , 1967, Plant physiology.

[3]  S. Izawa,et al.  Hill reaction rates and chloroplast fragment size. , 1965, Biochimica et biophysica acta.

[4]  B. Kok,et al.  The reducing power generated in photoact I of photosynthesis. , 1965, Biochimica et biophysica acta.

[5]  K. Sauer,et al.  Action spectra and quantum yields for nicotinamide--adenine dinucleotide phosphate reduction by chloroplasts. , 1965, Biochimica et biophysica acta.

[6]  D. Krogmann,et al.  HILL ACTIVITY IN CELL-FREE PREPARATIONS OF A BLUE-GREEN ALGA. , 1964, Biochimica et biophysica acta.

[7]  Raymond F. Jones,et al.  Studies on the Growth of the Red Alga Porphyridium cruentum , 1963 .

[8]  Russell J. Barrnett,et al.  Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation. , 1963 .

[9]  A. Jagendorf,et al.  Uncouplers of Spinach Chloroplast Photosynthetic Phosphorylation. , 1959, Plant physiology.

[10]  A. Jagendorf,et al.  Cofactors and rates of photosynthetic phosphorylation by spinach chloroplasts. , 1958, The Journal of biological chemistry.

[11]  J. Myers,et al.  NUTRITION AND GROWTH OF SEVERAL BLUE‐GREEN ALGAE , 1955 .

[12]  G. Mackinney,et al.  ABSORPTION OF LIGHT BY CHLOROPHYLL SOLUTIONS , 1941 .

[13]  John S. Burlew,et al.  Algal culture from laboratory to pilot plant. , 1953 .

[14]  D. Arnon.  COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. , 1949, Plant physiology.

[15]  K. Sauer,et al.  THE HILL REACTION OF CHLOROPLASTS ISOLATED FROM1 GLUTARALDEHYDE-FIXED SPINACH LEA VES* , 2022 .