Survival of enteroviruses and coliform bacteria in a sludge lagoon

Enteroviruses associate with aerobically and anaerobically digested sludge were determined before the addition of the sludge to a sludge lagoon. The fate of sludge-associated viruses was followed during detention of sludge in the lagoon and after application of sludge to land for disposal. While digested sludge was being added to the lagoon, enteroviruses were readily detected in grab samples of sludge from the lagoon. Sludge-associated viruses dropped to low or undetectable levels after disposal of sludge on land and during periods when addition of digested sludge to the lagoon was suspended. Changes in the levels of fecal coliforms in the lagooned sludge paralleled changes in the numbers of enteroviruses. Enteroviruses were not detected in water from deep wells located on the sludge disposal site or near the lagoon. During the initial part of the study, poliovirus serotypes accounted for greater than 90% of the viruses identified. Later, poliovirus serotypes comprised less than 40% of the virus isolates, and echoviruses and Coxsackieviruses were the most common enteroviruses identified.

[1]  E. Lund,et al.  Survival and movement of enterovirus in connection with land disposal of sludges , 1977 .

[2]  S. Sattar,et al.  Recovery of viruses from field samples of raw, digested, and lagoon-dried sludges. , 1979, Bulletin of the World Health Organization.

[3]  G. Gifford,et al.  Effect of a Secondary Treated Effluent on the Movement of Viruses through a Cypress Dome Soil 1 , 1976 .

[4]  M. Sobsey,et al.  Concentration of Enteroviruses from Large Volumes of Water , 1973, Applied microbiology.

[5]  E. Lund,et al.  The effect of sludge digestion on virus infectivity , 1977 .

[6]  C. Weber,et al.  Biological Field and Laboratory Methods for Measuring the Quality of Surface Waters and Effluents. Program Element 1BA027. , 1973 .

[7]  B. D. Hays.  Potential for parasitic disease transmission with land application of sewage plant effluents and sludges , 1977 .

[8]  L. Reed,et al.  A SIMPLE METHOD OF ESTIMATING FIFTY PER CENT ENDPOINTS , 1938 .

[9]  P. Kabler.  Removal of Pathogenic Microorganisms by Sewage Treatment Processes. , 1959 .

[10]  G. Bitton,et al.  Urea-lysine method for recovery of enteroviruses from sludge , 1981, Applied and environmental microbiology.

[11]  C. Gerba,et al.  Development of quantitative methods for the detection of enteroviruses in sewage sludges during activation and following land disposal , 1978, Applied and environmental microbiology.

[12]  G. Berg,et al.  Destruction by Anaerobic Mesophilic and Thermophilic Digestion of Viruses and Indicator Bacteria Indigenous to Domestic Sludges , 1980, Applied and environmental microbiology.

[13]  Charles P. Gerba,et al.  Fate of Wastewater Bacteria and Viruses in Soil , 1975 .

[14]  K. A. Lim,et al.  Typing of viruses by combinations of antiserum pools. Application to typing of enteroviruses (Coxsackie and ECHO). , 1960, Journal of immunology.