Hydrolysis of lactose in milk and whey with minute amounts of lactase

Summary A method for lactose hydrolysis in sterile milk or whey with Saccharomyces lactis lactase is described. The properties and stability of this enzyme are very favourable for use in milk and neutral whey. The long shelf life of sterilized milk and whey products is utilized for a prolonged incubation time – one to several weeks – at room temperature. Thus, minimal amounts of enzyme are needed which makes enzyme costs very low.