The Behaviour of Some Isometric Plant Viruses Heated in vitro as determined by Particle Stabilizing Forces

Summary Absorbance-temperature profiles, at 400 nm, of plant viruses with one or more kinds of isometric particle, were determined. Two temperatures were determined, Td (the temperature at which E 400 begins to increase) and Tf (the temperature at the inflexion point of the curve). The behaviour on heating at these temperatures was of two main types. Heating in vitro appears to be a useful means of making a gross subdivision of isometric plant viruses into two groups typified by cucumber mosaic virus and turnip yellow mosaic virus, respectively, in agreement with Kaper's (1971a) suggestion.

[1]  D. Gallitelli,et al.  Production of RNA and Artificial Top Component from Parsley Carrot-leaf Virus Heated In vitro , 1977 .

[2]  D. Gallitelli,et al.  Properties of Grapevine Fanleaf Virus , 1976 .

[3]  J. Kaper.  Arrangement and identification of simple isometric viruses according to their dominating stabilizing interactions. , 1973, Virology.

[4]  M. Mayo,et al.  Evidence for Two Functional RNA Species in Raspberry Ringspot Virus , 1972 .

[5]  T. O. Diener.  Potato spindle tuber "virus". IV. A replicating, low molecular weight RNA. , 1971, Virology.

[6]  J. Kaper,et al.  Interactions in biological polyelectrolytes: Studies on the dissociation and assembly of two simple RNA viruses , 1971 .

[7]  J. Kaper.  Studies on the stabilizing forces of simple RNA viruses. I. Selective interference with protein-RNA interactions in turnip yellow mosaic virus. , 1971, Journal of molecular biology.

[8]  T. O. Diener,et al.  Potato spindle tuber virus: a plant virus with properties of a free nucleic acid. I. Assay, extraction, and concentration. , 1969, Virology.

[9]  S. Spiegelman,et al.  Electrophoretic separation of viral nucleic acids on polyacrylamide gels. , 1967, Journal of molecular biology.

[10]  D. B. Dunn,et al.  THE USE OF BENTONITE IN THE PURIFICATION OF PLANT VIRUSES. , 1965, Virology.

[11]  L. Crawford,et al.  Biophysical studies of the interactions between the viruses of tobacco mosaic and tomato bushy stunt and their rabbit antisera. , 1960, Virology.

[12]  J. W. Lyttleton,et al.  Release of nucleic acid from turnip yellow mosaic virus under mild conditions. , 1958, Virology.

[13]  P. Kaesberg,et al.  The Molecular Weight and Other Biophysical Properties of Bromegrass Mosaic Virus. , 1962, Biophysical journal.