Mono-functional aminosilanes as primers for peptide functionalization.

When covalently attaching biomolecules to surfaces such as titanium, trifunctional silanes are commonly used as primers to produce surface amine groups. However, these primed surfaces are rarely uniform in structure due to networking of the silane. Mono-functional aminosilanes may result in more uniform structures, although their long-term stability and effect on osteoblast cell responses are possible issues for orthopedic applications. This study examines for the first time the optimization of peptide coupling to titanium using mono-functional aminosilane reaction chemistry. The resultant surface topography, chemistry, and thicknesses were characterized showing improved surface uniformity compared with trifunctional silanized surfaces. The stability of the coatings was examined over a period of 8 days in environments of varying pH, temperature, and humidity. In addition, human osteosarcoma (HOS) cell adhesion and spreading on the samples was examined; adhesion was minimal on silanized surfaces, but after functionalization with cysteine the cell density was greater than the titanium control and showed no overall detrimental effect on initial cell responses.

[1]  P. Dubruel,et al.  Comparative study of silanisation reactions for the biofunctionalisation of Ti-surfaces , 2006 .

[2]  Xiaowei Pei,et al.  Preparation and characterization of poly(N-isopropylacrylamide) films on a modified glass surface via surface initiated redox polymerization , 2005 .

[3]  R. Advíncula,et al.  Surface modification of surface sol-gel derived titanium oxide films by self-assembled monolayers (SAMs) and non-specific protein adsorption studies. , 2005, Colloids and surfaces. B, Biointerfaces.

[4]  R. Oréfice,et al.  Biomaterial with chemically engineered surface for protein immobilization , 2005, Journal of materials science. Materials in medicine.

[5]  S. Ek.  Controlled preparation of amino-functionalized surfaces on porous silica by atomic layer deposition , 2004 .

[6]  J. González‐Benito,et al.  Hydrolytic damage study of the silane coupling region in coated silica microfibres: pH and coating type effects , 2003 .

[7]  C. Tripp,et al.  Method to Double the Surface Concentration and Control the Orientation of Adsorbed (3-Aminopropyl)dimethylethoxysilane on Silica Powders and Glass Slides , 2002 .

[8]  C. Halliwell,et al.  A factorial analysis of silanization conditions for the immobilization of oligonucleotides on glass surfaces. , 2001, Analytical chemistry.

[9]  White,et al.  Reaction of (3-Aminopropyl)dimethylethoxysilane with Amine Catalysts on Silica Surfaces. , 2000, Journal of colloid and interface science.

[10]  R. Almeida,et al.  Densification of hybrid silica–titania sol–gel films studied by ellipsometry and FTIR , 2000 .

[11]  G. Vezon,et al.  Fourier transform infrared spectroscopic characterization of grafting of 3-aminopropyl silanol onto aluminum/alumina substrate. , 2000, Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy.

[12]  Gang-yu Liu,et al.  Correspondence between layer morphology and intralayer excitation transport dynamics in zirconium-phosphonate monolayers , 1999 .

[13]  Marcus Textor,et al.  Covalent Attachment of Cell-Adhesive, (Arg-Gly-Asp)-Containing Peptides to Titanium Surfaces , 1998 .

[14]  M. McKee,et al.  Chemical modification of titanium surfaces for covalent attachment of biological molecules. , 1998, Journal of biomedical materials research.

[15]  J. Finch,et al.  Silanation and stability of 3-aminopropyl triethoxy silane on nanosized superparamagnetic particles: I. Direct silanation , 1997 .

[16]  J. D. de Wijn,et al.  Biocompatibility screening of silane-treated hydroxyapatite powders, for use as filler in resorbable composites , 1996 .

[17]  Gil U. Lee,et al.  Covalent attachment of synthetic DNA to self-assembled monolayer films. , 1996, Nucleic acids research.

[18]  T. Groth,et al.  Reorganization of substratum-bound fibronectin on hydrophilic and hydrophobic materials is related to biocompatibility , 1994 .

[19]  P. Macdonald,et al.  Effect of Surface Water and Base Catalysis on the Silanization of Silica by (Aminopropyl)alkoxysilanes Studied by X-ray Photoelectron Spectroscopy and 13C Cross-Polarization/Magic Angle Spinning Nuclear Magnetic Resonance , 1994 .

[20]  Ingemar Lundström,et al.  Structure of 3-aminopropyl triethoxy silane on silicon oxide , 1991 .

[21]  R. Kellner,et al.  FTIR-ATR-spectroscopic investigation of the silanization of germanium surfaces with 3-aminopropyltriethoxysilane , 1989 .

[22]  G. Olofsson,et al.  Chemical vapour deposition of silanes , 1985 .

[23]  L. Merhari,et al.  Surface chemistry of TiO2 nanoparticles: influence on electrical and gas sensing properties , 2004 .

[24]  M. Delamar,et al.  Adsorption of aminopropyltriethoxy silane on quartz: an XPS and contact angle measurements study , 1996 .

[25]  D. Puleo.  Biochemical surface modification of Co-Cr-Mo. , 1996, Biomaterials.