论文信息 - Estimation of N Absorption and Secretion by Digesta Exchange between 15N Labelled and Unlabelled Pigs

Estimation of N Absorption and Secretion by Digesta Exchange between 15N Labelled and Unlabelled Pigs

Abstract Three female pigs (LW∼30 kg) were fitted with re-entrant cannulas in the duodenum and the ileum and with bladder catheters. Animal No. 1 was labelled by continuous infusion of [15N]leucine via a catheter into the vena jugularis. After reaching a steady state in the level of endogenous N (4–5 d after beginning of the infusion) the digesta of the labelled animal No. 1 and the two unlabelled animals were exchanged in a 3 day experiment. During this time the course of transit rates of digesta, digesta N and 15N through duodenum and ileum as well as the proportion of endogenous N: exogenous N were estimated. Using these data it was possible to calculate the secretion and absorption rates of endogenous and exogenous N in the three segments of the digestive tract: mouth-duodenum, duodenum-ileum, ileum-anus and in addition the reabsorption (intestinal recycling) of the endogenous N during its passage through the gut could be computed.

J. Kowalczyk | T. Żebrowska | R. Schadereit | K. Krawielitzki | F. Kreienbring

[1] J. Wünsche,et al. Determining of nitrogen absorption and nitrogen secretion in different sections of the pig's intestine by digesta exchange between 15N labelled and unlabelled animals. , 1990, Archiv fur Tierernahrung.

[2] J. Laplace,et al. Recycling of endogenous nitrogen in the pig (preliminary results of a collaborative study). , 1986, Archiv fur Tierernahrung.

[3] J. Partridge,et al. The passage and absorption of dietary and endogenous nitrogen in different regions of the digestive tract of rats given a single meal of 15N-labelled barley , 1985 .

[4] M. Ivan,et al. Exchange of digesta via duodenal cannula in sheep, a technique useful for absorption studies with labelled compounds. , 1982, Annales de recherches veterinaires. Annals of veterinary research.

[5] T. Żebrowska,et al. [Nitrogen and amino acid absorption in swine. 3. Endogenous secretion and absorption of nitrogen]. , 1978, Archiv fur Tierernahrung.

[6] T. Żebrowska,et al. [Studies on nitrogen and amino-acid absorption in the swine. Part 2. The course of 15 N labeling in the chyme, urine and blood]. , 1978, Archiv fur Tierernahrung.