Miscanthus: A Promising Biomass Crop

The C4 grass Miscanthus × giganteus is of increasing interest as a biomass feedstock for renewable fuel production. This review describes what is known to date on M. × giganteus from extensive research in Europe and more recently in the US. Research trials have shown that M. × giganteus productivity is among the highest recorded within temperate climates. The crop's high productivity results from greater levels of seasonal carbon fixation than other C4 crops during the growing season. Genetic sequencing of M. × giganteus has identified close homology with related crop species such as sorghum (Sorghum bicolor (L.) Moench) and sugarcane (Saccharum officinarum L.), and breeding of new varieties is underway. Miscanthus × giganteus has high water use efficiency; however, its exceptional productivity causes higher water use than other arable crops, potentially causing changes in hydrology in agricultural areas. Nitrogen use patterns are inconsistent and may indicate association with N fixing microorganisms. Miscanthus × giganteus has great promise as an economically and ecologically viable biomass crop; however, there are still challenges to widespread commercial development.

[1]  R. Costanza,et al.  SPECIAL ISSUE: The Dynamics and Value of Ecosystem Services: Integrating Economic and Ecological Perspectives Economic and ecological concepts for valuing ecosystem services , 2002 .

[2]  J. Greef,et al.  Syntaxonomy of Miscanthus x giganteus Greef et Deu , 1993 .

[3]  Enli Wang,et al.  Soil carbon change and its responses to agricultural practices in Australian agro-ecosystems: A review and synthesis , 2010 .

[4]  Mark W. Chase,et al.  Genetic resources of Miscanthus. , 2001 .

[5]  M. Acaroğlu,et al.  The cultivation and energy balance of Miscanthus×giganteus production in Turkey , 2005 .

[6]  H. Zub,et al.  Agronomic and physiological performances of different species of Miscanthus, a major energy crop. A review , 2010, Agronomy for Sustainable Development.

[7]  Ilia J Leitch,et al.  The use of dna sequencing (ITS and trnL-F), AFLP, and fluorescent in situ hybridization to study allopolyploid Miscanthus (Poaceae). , 2002, American journal of botany.

[8]  A. Masoni,et al.  Effect of irrigation and nitrogen fertilization on biomass yield and efficiency of energy use in crop production of Miscanthus , 1999 .

[9]  R. Perrin,et al.  Net energy of cellulosic ethanol from switchgrass , 2008, Proceedings of the National Academy of Sciences.

[10]  Halin Zhao,et al.  Seasonal pattern of antioxidant enzyme system in the roots of perennial forage grasses grown in alpine habitat, related to freezing tolerance , 2004 .

[11]  Iris Lewandowski,et al.  Delayed harvest of miscanthus—influences on biomass quantity and quality and environmental impacts of energy production , 2003 .

[12]  M. Margni,et al.  Considering time in LCA: dynamic LCA and its application to global warming impact assessments. , 2010, Environmental science & technology.

[13]  Stephen P. Long,et al.  Modification of the response of photosynthetic productivity to rising temperature by atmospheric CO2 concentrations: Has its importance been underestimated? , 1991 .

[14]  S. Polasky,et al.  Land Clearing and the Biofuel Carbon Debt , 2008, Science.

[15]  Ilias P. Tatsiopoulos,et al.  Logistics issues of biomass: The storage problem and the multi-biomass supply chain , 2009 .

[16]  Humberto Blanco-Canqui,et al.  Energy Crops and Their Implications on Soil and Environment , 2010 .

[17]  James D. McMillan,et al.  Pretreatment of lignocellulosic biomass , 1994 .

[18]  John Clifton-Brown,et al.  Screening Miscanthus genotypes in field trials to optimise biomass yield and quality in Southern Germany , 2002 .

[19]  J. R. Hess,et al.  Convergence of Agriculture and Energy: II. Producing Cellulosic Biomass for Biofuels , 2007 .

[20]  Nicolas Salamin,et al.  Phylogenetics of Miscanthus, Saccharum and related genera (Saccharinae, Andropogoneae, Poaceae) based on DNA sequences from ITS nuclear ribosomal DNA and plastid trnL intron and trnL-F intergenic spacers , 2002, Journal of Plant Research.

[21]  D. Wyse,et al.  Freezing tolerance of selected perennial ryegrass (Lolium perenne L.) accessions and its association with field winterhardiness and turf traits , 2008, Euphytica.

[22]  J. Scurlock,et al.  Miscanthus : European experience with a novel energy crop , 2000 .

[23]  Andrew H. Paterson,et al.  Genetic improvement of C4 grasses as cellulosic biofuel feedstocks , 2009, In Vitro Cellular & Developmental Biology - Plant.

[24]  Iris Lewandowski,et al.  Propagation method as an important factor in the growth and development of Miscanthus×giganteus , 1998 .

[25]  Stephen P. Long,et al.  Can perennial C4 grasses attain high efficiencies of radiant energy conversion in cool climates , 1995 .

[26]  Stephen P. Long,et al.  Leaf photosynthesis in the C4-grass Miscanthus x giganteus, growing in the cool temperate climate of southern England , 1996 .

[27]  N. O'neill,et al.  Miscanthus blight, a new foliar disease of ornamental grasses and sugarcane incited by Leptosphaeria sp. and its anamorphic state Stagonospora sp , 1996 .

[28]  Stephen L. Johnson,et al.  HYPOXIA IN THE NORTHERN GULF OF MEXICO , 2000 .

[29]  I. Holme,et al.  Callus induction and plant regeneration from different explant types of Miscanthus × ogiformis , 2022 .

[30]  S. Cosentino,et al.  Biomass yield and energy balance of three perennial crops for energy use in the semi-arid Mediterranean environment , 2009 .

[31]  J. Stier,et al.  Visualization of freezing progression in turfgrasses using infrared video thermography , 2003 .

[32]  J. Porter,et al.  The Physiology of Crop Yield , 2006 .

[33]  B. Jenkins,et al.  Combustion properties of biomass , 1998 .

[34]  D. G. Christian,et al.  The agronomy of some herbaceous crops grown for energy in Southern England. , 1997 .

[35]  I. Lewandowski,et al.  Comparing annual and perennial energy cropping systems with different management intensities , 2008 .

[36]  Fernando E. Miguez,et al.  Meta-analysis of the effects of management factors on Miscanthus × giganteus growth and biomass production , 2008 .

[37]  John Ralph,et al.  Plant biology and pathology / Biologie et pathologie végétales Genetic and molecular basis of grass cell-wall degradability. I. Lignin-cell wall matrix interactions ✩ , 2004 .

[38]  J. Porter,et al.  The Value of Producing Food, Energy, and Ecosystem Services within an Agro-Ecosystem , 2009, Ambio.

[39]  Virginia H. Dale,et al.  Hypoxia in the Northern Gulf of Mexico , 2010 .

[40]  S. Long,et al.  What is the maximum efficiency with which photosynthesis can convert solar energy into biomass? , 2008, Current opinion in biotechnology.

[41]  Pete Smith,et al.  The potential distribution of bioenergy crops in the UK under present and future climate , 2006 .

[42]  Stephen P. Long,et al.  The Productivity of the C_4 Grass Echinochloa Polystachya on the Amazon Floodplain , 1991 .

[43]  Sheryl A. Martin,et al.  Breaking the Biological barriers to Cellulosic Ethanol: A Joint Research Agenda , 2006 .

[44]  Stephen P. Long,et al.  Seasonal nitrogen dynamics of Miscanthus×giganteus and Panicum virgatum , 2009 .

[45]  F. Vivien,et al.  The convention on biological diversity: A conventionalist approach , 2005 .

[46]  John Clifton-Brown,et al.  The thermal response of leaf extension rate in genotypes of the C4-grass Miscanthus: an important factor in determining the potential productivity of different genotypes , 1997 .

[47]  É. Malézieux,et al.  Mixing plant species in cropping systems: concepts, tools and models. A review , 2011, Agronomy for Sustainable Development.

[48]  David S. Powlson,et al.  Biofuels and other approaches for decreasing fossil fuel emissions from agriculture , 2005 .

[49]  E. Heaton The Comparative Agronomic Potential of Miscanthus X Giganteus and Panicum Virgatum as Energy Crops in Illinois , 2006 .

[50]  C. Jung,et al.  Genetic diversity of European Miscanthus species revealed by AFLP fingerprinting , 1997, Genetic Resources and Crop Evolution.

[51]  A. Cropper Convention on Biological Diversity , 1993, Environmental Conservation.

[52]  Andrew B. Riche,et al.  Growth, yield and mineral content of Miscanthus × giganteus grown as a biofuel for 14 successive harvests , 2008 .

[53]  J. Hatfield,et al.  Nitrate-nitrogen patterns in the Raccoon River Basin related to agricultural practices , 2009, Journal of Soil and Water Conservation.

[54]  J. Morison,et al.  Water use efficiency of C4 perennial grasses in a temperate climate , 1999 .

[55]  David A. Blowers,et al.  Low growth temperatures modify the efficiency of light use by photosystem II for CO2 assimilation in leaves of two chilling-tolerant C4 species, Cyperus longus L. and Miscanthus x giganteus. , 2006, Plant, cell & environment.

[56]  John Clifton-Brown,et al.  Genotypic variation in cold tolerance influences the yield of Miscanthus , 2006 .

[57]  S. Anthony,et al.  Identifying the yield potential of Miscanthus x giganteus: an assessment of the spatial and temporal variability of M. x giganteus biomass productivity across England and Wales , 2004 .

[58]  MICHAEL B. Jones,et al.  Miscanthus for Renewable Energy Generation: European Union Experience and Projections for Illinois , 2004 .

[59]  R. Nagoshi,et al.  Development and Feeding of Fall Armyworm on Miscanihus × giganteus and Switchgrass , 2009, Journal of economic entomology.

[60]  G. Bollero,et al.  The ecology and agronomy of Miscanthus sinensis, a species important to bioenergy crop development, in its native range in Japan: a review , 2009 .

[61]  Carl J. Bernacchi,et al.  A comparison of canopy evapotranspiration for maize and two perennial grasses identified as potential bioenergy crops , 2010 .

[62]  D. D. Wolf,et al.  Switchgrass as a sustainable bioenergy crop , 1996 .

[63]  Michael E. Gray,et al.  Distribution, morphological description, and molecular characterization of Xiphinema and Longidorous spp. associated with plants (Miscanthus spp. and Panicum virgatum) used for biofuels , 2009 .

[64]  Stephen P. Long,et al.  More Productive Than Maize in the Midwest: How Does Miscanthus Do It?1[W][OA] , 2009, Plant Physiology.

[65]  Charlotte K. Williams,et al.  The Path Forward for Biofuels and Biomaterials , 2006, Science.

[66]  D. G. Christian Quantifying the yield of perennial grasses grown as a biofuel for energy generation , 1994 .

[67]  J. Widholm,et al.  The use of antimicrotubule herbicides for the production of doubled haploid plants from anther-derived maize callus , 1991, Theoretical and Applied Genetics.

[68]  Scott M. Swinton,et al.  Profitability Analysis of Cellulosic Energy Crops Compared with Corn , 2010 .

[69]  W. Huisman,et al.  Costs of supply chains of Miscanthus giganteus , 1997 .

[70]  S. Raghu,et al.  Refuge or Reservoir? The Potential Impacts of the Biofuel Crop Miscanthus x giganteus on a Major Pest of Maize , 2009, PloS one.

[71]  D. G. Christian,et al.  First report of barley yellow dwarf luteovirus onMiscanthus in the United Kingdom , 1994, European Journal of Plant Pathology.

[72]  G. McIsaac,et al.  Miscanthus and switchgrass production in central Illinois: impacts on hydrology and inorganic nitrogen leaching. , 2010, Journal of environmental quality.

[73]  Michael E. Gray,et al.  First Report of Field Populations of Two Potential Aphid Pests of the Bioenergy Crop Miscanthus × Giganteus , 2010 .

[74]  Wallace E. Tyner,et al.  The Global Impacts of Biofuel Mandates , 2010 .

[75]  Stephen Polasky,et al.  Climate change and health costs of air emissions from biofuels and gasoline , 2009, Proceedings of the National Academy of Sciences.

[76]  Salvador A. Gezan,et al.  Is UK biofuel supply from Miscanthus water‐limited? , 2008 .

[77]  Christopher J. Atkinson,et al.  Establishing perennial grass energy crops in the UK: A review of current propagation options for Miscanthus , 2009 .

[78]  Kevin J. Shinners,et al.  Fractional yield and moisture of corn stover biomass produced in the Northern US Corn Belt , 2007 .

[79]  F. Dohleman,et al.  Does greater leaf-level photosynthesis explain the larger solar energy conversion efficiency of Miscanthus relative to switchgrass? , 2009, Plant, cell & environment.

[80]  M. Walsh,et al.  Miscanthus : For Energy and Fibre , 2009 .

[81]  U. Nehls,et al.  Fungal carbohydrate support in the ectomycorrhizal symbiosis: a review. , 2010, Plant biology.

[82]  Fernando E. Miguez,et al.  A semimechanistic model predicting the growth and production of the bioenergy crop Miscanthus×giganteus: description, parameterization and validation , 2009 .

[83]  Philip W. Gassman,et al.  Impact of land use and land cover change on the water balance of a large agricultural watershed: Historical effects and future directions , 2008 .

[84]  M. Chase,et al.  Characterization of a genetic resource collection for Miscanthus (Saccharinae, Andropogoneae, Poaceae) using AFLP and ISSR PCR. , 2002, Annals of botany.

[85]  John Clifton-Brown,et al.  Water Use Efficiency and Biomass Partitioning of Three Different Miscanthus Genotypes with Limited and Unlimited Water Supply , 2000 .

[86]  J. Lammel,et al.  Cultivation of Miscanthus under West European conditions: Seasonal changes in dry matter production, nutrient uptake and remobilization , 1997, Plant and Soil.

[87]  John Clifton-Brown,et al.  Overwintering problems of newly established Miscanthus plantations can be overcome by identifying genotypes with improved rhizome cold tolerance , 2000 .

[88]  John Clifton-Brown,et al.  The modelled productivity of Miscanthus×giganteus (GREEF et DEU) in Ireland. , 2000 .

[89]  I. Holme,et al.  Callus induction and plant regeneration from different explant types of Miscanthus x ogiformis Honda ‘Giganteus’ , 1996, Plant Cell, Tissue and Organ Culture.

[90]  Michael Duffy,et al.  Estimated Costs of Crop Production in Iowa, 2014 , 2001 .

[91]  A. Troy,et al.  Valuing ecosystem services , 2010, Annals of the New York Academy of Sciences.

[92]  J. S. Cole Crop Rotation , 1944, Nature.

[93]  P. Ruckenbauer,et al.  The effect of fertilization on yield and quality of Miscanthus sinensis 'Giganteus'. , 1994 .

[94]  K. McDonnell,et al.  Nitrate leaching losses from Miscanthus × giganteus impact on groundwater quality. , 2009 .

[95]  Thomas B. Voigt,et al.  Effects of rhizome size, depth of planting and cold storage on Miscanthus x giganteus establishment in the Midwestern USA , 2010 .

[96]  Andrew B. Riche,et al.  Nitrate leaching losses under Miscanthus grass planted on a silty clay loam soil , 1998 .

[97]  P. Claassen,et al.  Pretreatment of Miscanthus for hydrogen production by Thermotoga elfii , 2002 .

[98]  R. Hatfield,et al.  Comparison of the acetyl bromide spectrophotometric method with other analytical lignin methods for determining lignin concentration in forage samples. , 2004, Journal of agricultural and food chemistry.

[99]  J. Houghton,et al.  Breaking the Biological Barriers to Cellulosic Ethanol: A Joint Research Agenda , 2006 .

[100]  D. Clark,et al.  The hydrological impacts of energy crop production in the UK. Final report , 2004 .

[101]  R. Perrin,et al.  Farm-Scale Production Cost of Switchgrass for Biomass , 2008, BioEnergy Research.

[102]  A. D. Schneider,et al.  Evapotranspiration, Yield, and Water Use Efficiency of Corn Hybrids Differing in Maturity , 1998 .

[103]  I. Mitsios,et al.  Potential growth and biomass productivity of Miscanthus×giganteus as affected by plant density and N-fertilization in central Greece , 2007 .

[104]  S. Long,et al.  Cool C4 Photosynthesis: Pyruvate Pi Dikinase Expression and Activity Corresponds to the Exceptional Cold Tolerance of Carbon Assimilation in Miscanthus × giganteus12[W][OA] , 2008, Plant Physiology.

[105]  V. Sundaresan,et al.  The indeterminate Gene Encodes a Zinc Finger Protein and Regulates a Leaf-Generated Signal Required for the Transition to Flowering in Maize , 1998, Cell.

[106]  R. Richards,et al.  Breeding Opportunities for Increasing the Efficiency of Water Use and Crop Yield in Temperate Cereals. , 2002, Crop science.

[107]  D. G. Christian,et al.  Performance of 15 Miscanthus genotypes at five sites in Europe , 2001 .

[108]  J. Clifton-Brown,et al.  Miscanthus biomass production for energy in Europe and its potential contribution to decreasing fossil fuel carbon emissions , 2004 .

[109]  Pamela J Green,et al.  Genomic and small RNA sequencing of Miscanthus × giganteus shows the utility of sorghum as a reference genome sequence for Andropogoneae grasses , 2010, Genome Biology.

[110]  P. Reich,et al.  Biodiversity and ecosystem stability in a decade-long grassland experiment , 2006, Nature.

[111]  A. Monti,et al.  Root distribution and soil moisture retrieval in perennial and annual energy crops in Northern Italy , 2009 .

[112]  D. Tilman,et al.  Carbon-Negative Biofuels from Low-Input High-Diversity Grassland Biomass , 2006, Science.

[113]  T. Crow,et al.  Agroecosystem restoration through strategic integration of perennials , 2006 .

[114]  B. Dale,et al.  Biofuels, land use change, and greenhouse gas emissions: some unexplored variables. , 2009, Environmental science & technology.

[115]  Martin J. Kropff,et al.  Crop modeling, QTL mapping, and their complementary role in plant breeding , 2003 .

[116]  P. Leinweber,et al.  Cropping of Miscanthus in Central Europe: biomass production and influence on nutrients and soil organic matter , 2001 .

[117]  Wilfred Vermerris,et al.  Miscanthus: Genetic resources and breeding potential to enhance bioenergy production , 2008 .

[118]  I. Linde-Laursen,et al.  Cytogenetic Analysis of Miscanthus‘Giganteus’, an Interspecific Hybrid , 2004 .

[119]  Dileep K. Birur,et al.  Biofuels and their By-Products: Global Economic and Environmental Implications , 2010 .

[120]  S. W. Humphries,et al.  WIMOVAC: a software package for modelling the dynamics of plant leaf and canopy photosynthesis , 1995, Comput. Appl. Biosci..

[121]  Shachar Lovett,et al.  Preface , 2012, COLT.

[122]  A. Hastings,et al.  Future energy potential of Miscanthus in Europe , 2009 .

[123]  K. Schwarz,et al.  Why do basic research? A lesson from commercial exploitation of miscanthus , 2000 .

[124]  P. Porter,et al.  Comment on "Carbon-Negative Biofuels from Low-Input High-Diversity Grassland Biomass" , 2007, Science.

[125]  R. Allen,et al.  Opportunities for Engineering Abiotic Stress Tolerance in Cotton Plants , 2010 .

[126]  S. Long,et al.  Can the cold tolerance of C4 photosynthesis in Miscanthus x giganteus relative to Zea mays be explained by differences in activities and thermal properties of Rubisco? , 2007, Journal of experimental botany.

[127]  John Clifton-Brown,et al.  Carbon mitigation by the energy crop, Miscanthus , 2007 .

[128]  Stephen P. Long,et al.  Perennial Grasses as Second-Generation Sustainable Feedstocks Without Conflict with Food Production , 2010 .

[129]  Mariam B. Sticklen,et al.  Feedstock crop genetic engineering for alcohol fuels. , 2007 .

[130]  B. Sobral,et al.  Phylogenetic analysis of chloroplast restriction enzyme site mutations in the Saccharinae Griseb. subtribe of the Andropogoneae Dumort. tribe , 1994, Theoretical and Applied Genetics.

[131]  A. Fares,et al.  Review of vadose zone soil solution sampling techniques , 2009 .

[132]  E. Kellogg,et al.  Molecular and morphological evolution in the Andropogoneae. , 2000 .

[133]  Uffe Jørgensen,et al.  Environment and harvest time affects the combustion qualities of Miscanthus genotypes , 2003 .

[134]  L. Reijnders Transport biofuel yields from food and lignocellulosic C4 crops , 2010 .

[135]  N. Carpita STRUCTURE AND BIOGENESIS OF THE CELL WALLS OF GRASSES. , 1996, Annual review of plant physiology and plant molecular biology.

[136]  Evan H. DeLucia,et al.  Comparative Biogeochemical Cycles of Bioenergy Crops Reveal Nitrogen-Fixation and Low Greenhouse Gas Emissions in a Miscanthus × giganteus Agro-Ecosystem , 2010, Ecosystems.

[137]  Ray Hurley,et al.  Statistics by subjects , 1956 .

[138]  M. J. Bullard,et al.  Principles of weed control in Miscanthus spp. under contrasting field conditions. , 1995 .

[139]  W. Huisman,et al.  Economical and technical comparison between herbaceous (Miscanthus x giganteus) and woody energy crops (Salix viminalis) , 1999 .

[140]  B. Solomon,et al.  Biofuels and sustainability , 2010, Annals of the New York Academy of Sciences.

[141]  J. Ditomaso,et al.  Nonnative Species and Bioenergy: Are We Cultivating the Next Invader? , 2008 .

[142]  Stephen P. Long,et al.  Meeting US biofuel goals with less land: the potential of Miscanthus , 2008 .

[143]  Stephen P. Long,et al.  Seasonal dynamics of nutrient accumulation and partitioning in the perennial C4-grasses Miscanthus × giganteus and Spartina cynosuroides , 1997 .

[144]  J. Finch,et al.  Interception losses from Miscanthus at a site in south‐east England—an application of the Gash model , 2010 .

[145]  Scott A. Staggenborg,et al.  Performance of Annual and Perennial Biofuel Crops: Yield during the First Two Years , 2010 .

[146]  Hyoung Seok Kim,et al.  Miscanthus×giganteus plant regeneration: effect of callus types, ages and culture methods on regeneration competence , 2010 .

[147]  J. Juvik,et al.  Genome Size of Three Miscanthus Species , 2009, Plant Molecular Biology Reporter.

[148]  R. Monson,et al.  16 – The Taxonomic Distribution of C4 Photosynthesis , 1999 .

[149]  A. Faaij,et al.  The impact of sustainability criteria on the costs and potentials of bioenergy production : applied for case studies in Brazil and Ukraine , 2010 .

[150]  Thomas B. Voigt,et al.  A quantitative review comparing the yields of two candidate C4 perennial biomass crops in relation to nitrogen, temperature and water , 2004 .

[151]  J. Allan Landscapes and Riverscapes: The Influence of Land Use on Stream Ecosystems , 2004 .

[152]  W. W. Nelson,et al.  Nitrate losses through subsurface tile drainage in Conservation Reserve Program, alfalfa, and row crop systems , 1997 .

[153]  A. Hastings,et al.  The development of MISCANFOR, a new Miscanthus crop growth model: towards more robust yield predictions under different climatic and soil conditions , 2009 .

[154]  Richard A Dixon,et al.  Lignin modification improves fermentable sugar yields for biofuel production , 2007, Nature Biotechnology.

[155]  W. Huisman,et al.  Mechanization of crop establishment, harvest, and post-harvest conservation of Miscanthus sinensis Giganteus , 1994 .

[156]  Thomas B. Voigt,et al.  Miscanthus × giganteus Response to Preemergence and Postemergence Herbicides , 2010, Weed Technology.

[157]  Steven R. Thomas,et al.  Herbaceous energy crop development: recent progress and future prospects. , 2008, Current opinion in biotechnology.

[158]  A. Kicherer,et al.  Combustion quality of biomass: practical relevance and experiments to modify the biomass quality of Miscanthus x giganteus , 1997 .

[159]  James W. Jones,et al.  POTENTIAL USES AND LIMITATIONS OF CROP MODELS , 1996 .

[160]  Erin M. Tegtmeier,et al.  External Costs of Agricultural Production in the United States , 2004 .

[161]  Li Tao,et al.  Utilization of miscanthus and development strategy. , 2010 .

[162]  D. Mohan,et al.  Pyrolysis of Wood/Biomass for Bio-oil: A Critical Review , 2006 .

[163]  G. A. Little The Pew Charitable Trusts , 1994 .

[164]  F. Dohleman,et al.  Agronomic experiences with Miscanthus x giganteus in Illinois, USA. , 2009, Methods in molecular biology.

[165]  Jakob Magid,et al.  Decomposition of plant residues at low temperatures separates turnover of nitrogen and energy rich tissue components in time , 2004, Plant and Soil.

[166]  Andrew D. Hanson,et al.  Whole-Plant Response to Water Deficits: Water Deficits and the Nitrogen Economy , 1983 .

[167]  Richard E. Joost,et al.  Biorenewable Resources, Engineering New Products from Agriculture , 2004 .

[168]  R Arundale,et al.  First Report of Pithomyces chartarum Causing a Leaf Blight of Miscanthus × giganteus in Kentucky. , 2010, Plant disease.

[169]  J. Scurlock,et al.  The development and current status of perennial rhizomatous grasses as energy crops in the US and Europe , 2003 .

[170]  Tamas Lelley,et al.  Cytogenetic Studies of Different Miscanthus Species with Potential for Agricultural Use , 1994 .

[171]  Genera graminum: Grasses of the World , 1987 .

[172]  O. Olsson,et al.  Increased gibberellin biosynthesis in transgenic trees promotes growth, biomass production and xylem fiber length , 2000, Nature Biotechnology.

[173]  Hyoung Seok Kim,et al.  Chromosome doubling of the bioenergy crop, Miscanthus×giganteus , 2009 .

[174]  A. Faaij,et al.  Bioenergy revisited: Key factors in global potentials of bioenergy , 2010 .

[175]  R. Sage,et al.  The temperature response of C(3) and C(4) photosynthesis. , 2007, Plant, cell & environment.

[176]  R. Hartley,et al.  Occurrence and nature of ferulic acid substitution of cell-wall polysaccharides in graminaceous plants , 1983 .

[177]  J. Lammel,et al.  Spatial and temporal distribution of the root system and root nutrient content of an established Miscanthus crop , 1999 .

[178]  I. Lewandowski,et al.  CO2-balance for the cultivation and combustion of Miscanthus , 1995 .

[179]  S. Datta Impact of Plant Biotechnology in Agriculture , 2007 .

[180]  Simon R. Leather,et al.  Suitability of the biomass crop Miscanthus sinensis as a host for the aphids Rhopalosiphum padi (L.) and Rhopalosiphum maidis (F.), and its susceptibility to the plant luteovirus Barley Yellow Dwarf Virus , 1999 .

[181]  Iris Lewandowski,et al.  Nitrogen, energy and land use efficiencies of miscanthus, reed canary grass and triticale as determined by the boundary line approach , 2006 .

[182]  Robert G. Hartzler,et al.  Weed management in short rotation poplar and herbaceous perennial crops grown for biofuel production , 1998 .

[183]  J. Fike,et al.  The Biology and Agronomy of Switchgrass for Biofuels , 2005 .

[184]  D. Andow,et al.  Multifunctional Agriculture in the United States , 2005 .

[185]  A. Faaij,et al.  The economical and environmental performance of miscanthus and switchgrass production and supply chains in a European setting , 2009 .