A label-free strategy for facile electrochemical analysis of dynamic glycan expression on living cells.

A novel, label-free strategy has been developed for facile electrochemical analysis of dynamic glycan expression on living cells, which uses carbon nanohorns to efficiently immobilize lectin for the construction of a recognition interface and enhancing accessibility of cell surface glycan motifs.

[1]  Lloyd M Smith,et al.  Lectin arrays for profiling cell surface carbohydrate expression. , 2005, Journal of the American Chemical Society.

[2]  C. Bertozzi,et al.  Glycans in cancer and inflammation — potential for therapeutics and diagnostics , 2005, Nature Reviews Drug Discovery.

[3]  Lloyd M. Smith,et al.  Analysis of cell surface carbohydrate expression patterns in normal and tumorigenic human breast cell lines using lectin arrays. , 2007, Analytical chemistry.

[4]  C. Gahmberg,et al.  Differentiation of human erythroid cells is associated with increased O-glycosylation of the major sialoglycoprotein, glycophorin A. , 1984, Proceedings of the National Academy of Sciences of the United States of America.

[5]  Xin Liu,et al.  Mass spectrometry-based glycomics strategy for exploring N-linked glycosylation in eukaryotes and bacteria. , 2006, Analytical chemistry.

[6]  H. Ju,et al.  Carbohydrate monolayer strategy for electrochemical assay of cell surface carbohydrate. , 2008, Journal of the American Chemical Society.

[7]  Lara K Mahal,et al.  A ratiometric lectin microarray approach to analysis of the dynamic mammalian glycome , 2007, Proceedings of the National Academy of Sciences.

[8]  J. Paulson,et al.  Glycomics: an integrated systems approach to structure-function relationships of glycans , 2005, Nature Methods.

[9]  Satoshi Fukuoka,et al.  Fabrication of quantum dot-lectin conjugates as novel fluorescent probes for microscopic and flow cytometric identification of leukemia cells from normal lymphocytes. , 2005, Chemical communications.

[10]  M. Yudasaka,et al.  Light-assisted oxidation of single-wall carbon nanohorns for abundant creation of oxygenated groups that enable chemical modifications with proteins to enhance biocompatibility. , 2007, ACS nano.

[11]  Yanbin Li,et al.  Interdigitated Array microelectrode-based electrochemical impedance immunosensor for detection of Escherichia coli O157:H7. , 2004, Analytical chemistry.

[12]  N. Sharon,et al.  Lectins: Carbohydrate-Specific Proteins That Mediate Cellular Recognition. , 1998, Chemical reviews.

[13]  H. Ju,et al.  Effective cell capture with tetrapeptide-functionalized carbon nanotubes and dual signal amplification for cytosensing and evaluation of cell surface carbohydrate. , 2008, Analytical chemistry.

[14]  I. Hamachi,et al.  Double-modification of lectin using two distinct chemistries for fluorescent ratiometric sensing and imaging saccharides in test tube or in cell. , 2005, Journal of the American Chemical Society.

[15]  A. Kuno,et al.  A novel strategy for mammalian cell surface glycome profiling using lectin microarray. , 2007, Glycobiology.

[16]  A. Dell,et al.  Carbohydrate structure of erythropoietin expressed in Chinese hamster ovary cells by a human erythropoietin cDNA. , 1987, Journal of Biological Chemistry.

[17]  H. Ju,et al.  Sandwich nanohybrid of single-walled carbon nanohorns-TiO2-porphyrin for electrocatalysis and amperometric biosensing towards chloramphenicol. , 2009, Chemical communications.

[18]  Wei Cheng,et al.  A simple electrochemical cytosensor array for dynamic analysis of carcinoma cell surface glycans. , 2009, Angewandte Chemie.

[19]  Lara K Mahal,et al.  Deciphering the glycocode: the complexity and analytical challenge of glycomics. , 2007, Current opinion in chemical biology.

[20]  A. Oratore,et al.  Pattern expression of glycan residues in AZT-treated K562 cells analyzed by lectin cytochemistry , 2007, Molecular and Cellular Biochemistry.

[21]  Ku-Lung Hsu,et al.  Analyzing the dynamic bacterial glycome with a lectin microarray approach , 2006, Nature chemical biology.