Effects of varying dietary protein levels on growth, reproductive performance, body and egg composition of rohu, Labeo rohita (Hamilton)

A 360-day feeding trial was conducted to observe the influence of varying levels of dietary protein on growth, reproductive performance, body and egg composition of rohu, Labeo rohita. Twenty fish (40.4 ± 0.24 cm; 852 ± 4.9 g), stocked in outdoor concrete tanks (200 m2), in duplicate, were fed diets with varying levels (200, 250, 300, 350 and 400 g kg−1) of crude protein exchanged with carbohydrate to apparent satiation, twice daily, at 09:00 and 17:00 h. Higher (P   0.05) among fish of different dietary groups except those fed 200 g kg−1 protein diet which produced least values. Egg diameter remained unaffected (P > 0.05) by variations in levels of dietary protein. Relative fecundity was maximum (P   0.05) by variations in dietary protein level. Hatchability (%) followed the trend of variations almost similar to that of fertilizability. Proximate composition of muscle and eggs varied significantly (P < 0.05) with dietary protein levels. For broodstock L. rohita, a dietary protein level of 250 g kg−1 was found optimum with regard to its reproductive performance, egg quality and composition.

[1]  John P. Sumpter,et al.  Egg quality in fish: what makes a good egg? , 1997, Reviews in Fish Biology and Fisheries.

[2]  G. Mourente,et al.  Effect of broodstock diets on lipid classes and their fatty acid composition in eggs of gilthead sea bream (Sparus aurata L.) , 1990, Fish Physiology and Biochemistry.

[3]  A. Tacon,et al.  Effect of broodstock nutrition on reproductive performance of fish , 2001 .

[4]  Blanc,et al.  Effects of dietary keto‐carotenoids (canthaxanthin and astaxanthin) on the reproductive performance of female rainbow trout Oncorhynchus mykiss (Walbaum) , 1998 .

[5]  M. Reis-Henriques,et al.  Effect of protein source on the reproductive performance of female rainbow trout, Oncorhynchus mykiss (Walbaum) , 1998 .

[6]  Y. Al-Hafedh,et al.  Effect of dietary protein level on the reproductive performance of Nile tilapia, Oreochromis niloticus (L.) , 1998 .

[7]  O. Torrissen,et al.  Effects of dietary astaxanthin supplementation on fertilization and egg survival in Atlantic salmon (Salmo salar L.) , 1997 .

[8]  T. J. Lam,et al.  Influence of dietary protein content on the distribution of amino acids in oocytes, serum and muscle of Nile tilapia, Oreochromis niloticus (L.) , 1997 .

[9]  R. Gunasekera,et al.  Influence of dietary protein level on ovarian recrudescence in Nile tilapia, Oreochromis niloticus (L.) , 1997 .

[10]  M. Salhi,et al.  The effect of dietary protein and lipid from squid and fish meals on egg quality of broodstock for gilthead seabream (Sparus aurata) , 1997 .

[11]  T. J. Lam,et al.  Influence of protein content of broodstock diets on larval quality and performance in Nile tilapia, Oreochromis niloticus (L.) , 1996 .

[12]  R. Gunasekera,et al.  Effect of dietary protein level on spawning performance and amino acid composition of eggs of Nile tilapia, Oreochromis niloticus , 1996 .

[13]  R. Singh,et al.  Effect of formulated diets on the growth and ovarian maturation in common carp Cyprinus carpio (!ommunis Linn. , 1996 .

[14]  T. J. Lam,et al.  Effect of dietary protein level on puberty, oocyte growth and egg chemical composition in the tilapia, Oreochromis niloticus (L.) , 1995 .

[15]  M. Salhi,et al.  Effect of n − 3 HUFA level in broodstock diets on egg quality of gilthead sea bream (Sparus aurata L.) , 1995 .

[16]  K. Dąbrowski,et al.  Reproductive success of female rainbow trout (Oncorhynchus mykiss) in response to graded dietary ascorbyl monophosphate levels. , 1995, Biology of reproduction.

[17]  J. Cerdà,et al.  Influence of nutritional composition of diet on sea bass, Dicentrarchus labrax L., reproductive performance and egg and larval quality , 1994 .

[18]  G. Choubert,et al.  Muscle pigmentation changes during and after spawning in male and female rainbow trout, Oncorhynchus mykiss, fed dietary carotenoids , 1993 .

[19]  C. B. Santiago,et al.  Effects of dietary lipid source on reproductive performance and tissue lipid levels of Nile tilapia Oreochromis niloticus (Linnaeus) broodstock , 1993 .

[20]  M. A. Laron,et al.  Growth and reproductive performance of bighead carp (Aristichthys nobilis) reared with or without feeding in floating cages , 1991 .

[21]  S. O. Hung,et al.  Dietary effects on tissue composition, oogenesis and the reproductive performance of female rainbow trout (Oncorhynchus mykiss). , 1990 .

[22]  P. Eskelinen.  Effects of different diets on egg production and egg quality of Atlantic salmon (Salmo salar L.) , 1989 .

[23]  J. Metcoff.  Intracellular amino acid levels as predictors of protein synthesis. , 1986, Journal of the American College of Nutrition.

[24]  A. Itoh,et al.  Nutritional studies in the seed production of fish - XVIII. Effect of dietary protein levels and feeding period before spawning on chemical components of eggs produced by Red Sea bream broodstock. , 1985 .

[25]  R. Hardy,et al.  Proximate and elemental composition of developing eggs and maternal soma of pen-reared coho salmon (Oncorhynchus kisutch) fed production and trace element fortified diets☆☆☆ , 1984 .

[26]  A. Itoh,et al.  Nutritional studies in the seed production of fish - XV. Effect of nutritional composition of diets on chemical components of red sea bream broodstock and eggs produced. , 1984 .

[27]  A. Itoh,et al.  Nutritional studies in the seed production of fish - XVI. Effect of dietary protein levels on reproduction of red sea bream. , 1984 .

[28]  K. Jauncey.  The effects of varying dietary protein level on the growth, food conversion, protein utilization and body composition of juvenile tilapias (Sarotherodon mossambicus) , 1982 .

[29]  B. Dahlgren.  The effects of three different dietary protein levels on the fecundity in the guppy, Poecilia reticulata (Peters). , 1980 .

[30]  D. B. Duncan.  MULTIPLE RANGE AND MULTIPLE F TESTS , 1955 .