Molecular clones of the mouse t complex derived from microdissected metaphase chromosomes

Fragments of the proximal half of mouse chromosome 17 including the t-complex region were microdissected from metaphase spreads. DNA was isolated from a pool of such fragments, and was cloned on microscale. Individual clones were used to probe genomic digests of DNA from a pair of Chinese hamster cell lines with or without mouse chromosome 17, and livers of congenic inbred lines of mice carrying wild-type and/or t-haplotype forms of chromosome 17. The data obtained indicate that 95% of the low copy number microclone inserts recognize DNA sequences present on mouse chromosome 17. It has been possible to use one-third of these clones to identify restriction-fragment-length polymorphisms between wild-type and t-haplotype DNA on a congenic background. These results demonstrate that these clones have been derived from the t-complex or regions closely linked to it. Clones of this type should provide starting points for a molecular analysis of this region of the mouse genome.

[1]  J. Vieira,et al.  The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. , 1982, Gene.

[2]  Margaret C. Green,et al.  Genetic variants and strains of the laboratory mouse , 1981 .

[3]  M. Lyon,et al.  t-Haplotypes of the mouse may involve a change in intercalary DNA , 1979, Nature.

[4]  M. Skolnick,et al.  Strategies for detecting and characterizing restriction fragment length polymorphisms (RFLP's). , 1982, Cytogenetics and cell genetics.

[5]  L. Silver.  Genomic analysis of the H-2 complex region associated with mouse t Haplotypes , 1982, Cell.

[6]  A. Poustka,et al.  Lambda replacement vectors carrying polylinker sequences. , 1983, Journal of molecular biology.

[7]  P. D’Eustachio,et al.  Dispersion of α-like globin genes of the mouse to three different chromosomes , 1981, Nature.

[8]  N. Hillman,et al.  ATP metabolism in tn/tn mouse embryos. , 1975, Journal of embryology and experimental morphology.

[9]  J. Forejt,et al.  T(16: 17)43H translocation as a tool in analysis of the proximal part of chromosome 17 (including T-t gene complex) of the mouse. , 1980, Genetical research.

[10]  L. Silver,et al.  Recombination suppression of mouse t-haplotypes due to chromatin mismatching , 1981, Nature.

[11]  R. Lathe,et al.  Overproduction of a viral protein during infection of a lyc mutant of Escherichia coli with phage lambdamm434. , 1977, Virology.

[12]  E. Eicher,et al.  Sex reversal in XY mice caused by dominant mutation on chromosome 17 , 1983, Nature.

[13]  D. Röhme.  Evidence for a relationship between longevity of mammalian species and life spans of normal fibroblasts in vitro and erythrocytes in vivo. , 1981, Proceedings of the National Academy of Sciences of the United States of America.

[14]  F. Kao,et al.  Isolation and chromosomal localization of unique DNA sequences from a human genomic library. , 1982, Proceedings of the National Academy of Sciences of the United States of America.

[15]  L. Silver.  Genetic organization of the mouse t complex , 1981, Cell.

[16]  J. Klein,et al.  The Control of Differentiation by the T Complex , 1977, Immunological reviews.

[17]  L. Silver,et al.  A diversified set of testicular cell proteins specified by genes within the mouse t complex , 1983, Cell.

[18]  J. Yunis,et al.  Chromosomes and cancer: new nomenclature and future directions. , 1981, Human pathology.

[19]  K. Artzt,et al.  Genetic structure and origin of t haplotypes of mice, analyzed with H-2 cDNA probes , 1982, Cell.

[20]  E. Lewis,et al.  Molecular Genetics of the Bithorax Complex in Drosophila melanogaster , 1983, Science.

[21]  S. Latt,et al.  Regional localization on the human X of DNA segments cloned from flow sorted chromosomes. , 1982, Nucleic acids research.

[22]  K. Davies,et al.  The occurrence of families of repetitive sequences in a library of cloned cDNA from human lymphocytes. , 1981, Nucleic acids research.

[23]  D. Housman,et al.  Isolation and localization of DNA segments from specific human chromosomes. , 1980, Proceedings of the National Academy of Sciences of the United States of America.

[24]  D. Bennett.  The T-locus of the mouse , 1975, Cell.

[25]  K. Davies,et al.  Cloning of a representative genomic library of the human X chromosome after sorting by flow cytometry , 1981, Nature.