A single protein based nanobiosensor for odorant recognition

We propose a nanosensor with a biological active part able to identify specific odorants. The biological part should be constituted by olfactory receptors pertaining to the G protein-coupled receptors, the most efficient natural sensors for odorant discrimination. Modeling, design, and experiments performed for proving the concept are reported and discussed.

[1]  David Cahen,et al.  Bacteriorhodopsin‐Monolayer‐Based Planar Metal–Insulator–Metal Junctions via Biomimetic Vesicle Fusion: Preparation, Characterization, and Bio‐optoelectronic Characteristics , 2007 .

[2]  P De Los Rios,et al.  Discrete breathers in nonlinear network models of proteins. , 2007, Physical review letters.

[3]  T. N. Bhat,et al.  The Protein Data Bank , 2000, Nucleic Acids Res..

[4]  Nagarajan Vaidehi,et al.  Predicted 3-D structures for mouse I7 and rat I7 olfactory receptors and comparison of predicted odor recognition profiles with experiment. , 2004, Chemical senses.

[5]  T. Blundell,et al.  Comparative protein modelling by satisfaction of spatial restraints. , 1993, Journal of molecular biology.

[6]  J Samitier,et al.  Nanoscale electrical conductivity of the purple membrane monolayer. , 2007, Physical review. E, Statistical, nonlinear, and soft matter physics.

[7]  Peter Mombaerts,et al.  Odorant receptor gene choice in olfactory sensory neurons: the one receptor–one neuron hypothesis revisited , 2004, Current Opinion in Neurobiology.

[8]  Karine Badonnel,et al.  On a chip demonstration of a functional role for Odorant Binding Protein in the preservation of olfactory receptor activity at high odorant concentration. , 2008, Lab on a chip.

[9]  D Schild,et al.  Bioelectronic noses: a status report. Part I. , 1998, Biosensors & bioelectronics.

[10]  Lino Reggiani,et al.  Charge transport in bacteriorhodopsin monolayers: The contribution of conformational change to current-voltage characteristics , 2008, 0804.1720.

[11]  Tirion,et al.  Large Amplitude Elastic Motions in Proteins from a Single-Parameter, Atomic Analysis. , 1996, Physical review letters.

[12]  J Samitier,et al.  Electron transport through supported biomembranes at the nanoscale by conductive atomic force microscopy , 2007, Nanotechnology.

[13]  Josep Samitier,et al.  Immobilization of rhodopsin on a self-assembled multilayer and its specific detection by electrochemical impedance spectroscopy. , 2006, Biosensors & bioelectronics.

[14]  Marta Filizola,et al.  Modeling activated states of GPCRs: the rhodopsin template , 2007, J. Comput. Aided Mol. Des..

[15]  Qingjun Liu,et al.  Olfactory cell-based biosensor: a first step towards a neurochip of bioelectronic nose. , 2006, Biosensors & bioelectronics.

[16]  M. F.,et al.  Bibliography , 1985, Experimental Gerontology.

[17]  Josep Samitier,et al.  A novel detection strategy for odorant molecules based on controlled bioengineering of rat olfactory receptor I7. , 2007, Biosensors & bioelectronics.

[18]  Josep Samitier,et al.  Towards the Realization of Nanobiosensors Based on G‐protein‐coupled Receptors , 2007 .

[19]  P. Duchamp-Viret,et al.  Odor response properties of rat olfactory receptor neurons. , 1999, Science.

[20]  Lino Reggiani,et al.  Topological change and impedance spectrum of rat olfactory receptor I7: A comparative analysis with , 2009 .

[21]  András Fiser,et al.  ModLoop: automated modeling of loops in protein structures , 2003, Bioinform..

[22]  Peter L. Freddolino,et al.  Prediction of structure and function of G protein-coupled receptors , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[23]  R. Axel,et al.  A novel multigene family may encode odorant receptors: A molecular basis for odor recognition , 1991, Cell.