Gene targeting in primary human trophoblasts.

[1]  Y. Kanai,et al.  Mammalian target of rapamycin signalling modulates amino acid uptake by regulating transporter cell surface abundance in primary human trophoblast cells , 2013, The Journal of physiology.

[2]  A. Pasquinelli MicroRNAs and their targets: recognition, regulation and an emerging reciprocal relationship , 2012, Nature Reviews Genetics.

[3]  Lu Gao,et al.  Differential regulation of glucose transporters mediated by CRH receptor type 1 and type 2 in human placental trophoblasts. , 2012, Endocrinology.

[4]  L. Groome,et al.  ADAM17 regulates TNFα production by placental trophoblasts. , 2011, Placenta.

[5]  Ben Berkhout,et al.  miRNA cassettes in viral vectors: problems and solutions. , 2011, Biochimica et biophysica acta.

[6]  Sindy Sohr,et al.  The tumor suppressor p53 induces expression of the pregnancy-supporting human chorionic gonadotropin (hCG) CGB7 gene , 2011, Cell cycle.

[7]  P. Leung,et al.  Twist regulates cadherin-mediated differentiation and fusion of human trophoblastic cells. , 2011, The Journal of clinical endocrinology and metabolism.

[8]  Y. Taketani,et al.  Toll-like receptor-3 ligation-induced indoleamine 2, 3-dioxygenase expression in human trophoblasts. , 2011, Endocrinology.

[9]  Chih-ping Chen,et al.  Spatiotemporal expression of SERPINE2 in the human placenta and its role in extravillous trophoblast migration and invasion , 2011, Reproductive biology and endocrinology : RB&E.

[10]  Ching-Wen Chang,et al.  A Novel Cyclic AMP/Epac1/CaMKI Signaling Cascade Promotes GCM1 Desumoylation and Placental Cell Fusion , 2011, Molecular and Cellular Biology.

[11]  C. Mendelson,et al.  Estrogen-related receptor gamma (ERRgamma) mediates oxygen-dependent induction of aromatase (CYP19) gene expression during human trophoblast differentiation. , 2011, Molecular endocrinology.

[12]  J. Kingdom,et al.  Glial cell missing-1 mediates over-expression of tissue inhibitor of metalloproteinase-4 in severe pre-eclamptic placental villi. , 2011, Human reproduction.

[13]  Y. Sadovsky,et al.  Fatty acid binding protein 4 regulates intracellular lipid accumulation in human trophoblasts. , 2011, The Journal of clinical endocrinology and metabolism.

[14]  Pankaj Kumar,et al.  Optimization of lentiviral vectors generation for biomedical and clinical research purposes: contemporary trends in technology development and applications. , 2011, Current gene therapy.

[15]  L. Myatt,et al.  The Sp1 transcription factor is crucial for the expression of 11beta-hydroxysteroid dehydrogenase type 2 in human placental trophoblasts. , 2011, The Journal of clinical endocrinology and metabolism.

[16]  I. Crocker,et al.  IGF2 Actions on Trophoblast in Human Placenta Are Regulated by the Insulin-Like Growth Factor 2 Receptor, Which Can Function as Both a Signaling and Clearance Receptor1 , 2011, Biology of reproduction.

[17]  S. Lye,et al.  Nodal signals through activin receptor-like kinase 7 to inhibit trophoblast migration and invasion: implication in the pathogenesis of preeclampsia. , 2011, The American journal of pathology.

[18]  C. Fiala,et al.  Transcription factor AP-2α promotes EGF-dependent invasion of human trophoblast. , 2011, Endocrinology.

[19]  H. Jäck,et al.  A facile method to increase titers of miRNA‐encoding retroviruses by inhibition of the RNaseIII enzyme Drosha , 2011, European journal of immunology.

[20]  M. Westwood,et al.  A limitation of the method for siRNA delivery into primary human cytotrophoblast cells. , 2011, Placenta.

[21]  A. Cimarelli,et al.  The Inside Out of Lentiviral Vectors , 2011, Viruses.

[22]  P. Mark,et al.  Placental ABCA1 and ABCG1 transporters efflux cholesterol and protect trophoblasts from oxysterol induced toxicity. , 2010, Biochimica et biophysica acta.

[23]  Theresa A. Storm,et al.  Argonaute proteins are key determinants of RNAi efficacy, toxicity, and persistence in the adult mouse liver. , 2010, The Journal of clinical investigation.

[24]  C. Sibley,et al.  The contribution of SNAT1 to system A amino acid transporter activity in human placental trophoblast , 2010, Biochemical and biophysical research communications.

[25]  B. Berkhout,et al.  Titers of lentiviral vectors encoding shRNAs and miRNAs are reduced by different mechanisms that require distinct repair strategies. , 2010, RNA.

[26]  J. Guibourdenche,et al.  ZO-1 is involved in trophoblastic cell differentiation in human placenta. , 2010, American journal of physiology. Cell physiology.

[27]  Y. Sadovsky,et al.  MiR‐205 silences MED1 in hypoxic primary human trophoblasts , 2010, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[28]  Nicholas T. Ingolia,et al.  Mammalian microRNAs predominantly act to decrease target mRNA levels , 2010, Nature.

[29]  I. Sargent,et al.  Downregulation of Caveolin-1 Enhances Fusion of Human BeWo Choriocarcinoma Cells , 2010, PloS one.

[30]  M. Westwood,et al.  Transforming growth factor-{beta} (TGF{beta}) receptors I/II differentially regulate TGF{beta}1 and IGF-binding protein-3 mitogenic effects in the human placenta. , 2010, Endocrinology.

[31]  R. Kreienberg,et al.  Differential expression of VE-cadherin and VEGFR2 in placental syncytiotrophoblast during preeclampsia - New perspectives to explain the pathophysiology. , 2010, Placenta.

[32]  S. Handwerger,et al.  Involvement of Transcription Factor NR2F2 in Human Trophoblast Differentiation , 2010, PloS one.

[33]  T. Powell,et al.  Full-Length Adiponectin Attenuates Insulin Signaling and Inhibits Insulin-Stimulated Amino Acid Transport in Human Primary Trophoblast Cells , 2010, Diabetes.

[34]  A. P. Bol’shakov,et al.  Transfection of Nerve Cells , 2010, Neuroscience and Behavioral Physiology.

[35]  S. Zhou,et al.  Effect of 14-3-3 tau protein on differentiation in BeWo choriocarcinoma cells. , 2010, Placenta.

[36]  P. Linsley,et al.  Recognizing and avoiding siRNA off-target effects for target identification and therapeutic application , 2010, Nature Reviews Drug Discovery.

[37]  J. Aplin Developmental cell biology of human villous trophoblast: current research problems. , 2010, The International journal of developmental biology.

[38]  H. Wang,et al.  The proprotein convertase furin in human trophoblast: Possible role in promoting trophoblast cell migration and invasion. , 2009, Placenta.

[39]  T. Powell,et al.  IL-6 stimulates system A amino acid transporter activity in trophoblast cells through STAT3 and increased expression of SNAT2. , 2009, American journal of physiology. Cell physiology.

[40]  M. Westwood,et al.  The protein-tyrosine phosphatase, SRC homology-2 domain containing protein tyrosine phosphatase-2, is a crucial mediator of exogenous insulin-like growth factor signaling to human trophoblast. , 2009, Endocrinology.

[41]  C. Cadwell,et al.  Ablation of Stat3 by siRNA alters gene expression profiles in JEG-3 cells: a systems biology approach. , 2009, Placenta.

[42]  H. Guan,et al.  The p38 mitogen-activated protein kinase regulates 11beta-hydroxysteroid dehydrogenase type 2 (11beta-HSD2) expression in human trophoblast cells through modulation of 11beta-HSD2 messenger ribonucleic acid stability. , 2009, Endocrinology.

[43]  S. Landry,et al.  Syncytin-2 plays an important role in the fusion of human trophoblast cells. , 2009, Journal of molecular biology.

[44]  E. Schleußner,et al.  mTOR mediates human trophoblast invasion through regulation of matrix-remodeling enzymes and is associated with serine phosphorylation of STAT3. , 2009, Experimental cell research.

[45]  A. Judge,et al.  siRNA and innate immunity. , 2009, Oligonucleotides.

[46]  M. Bally,et al.  siRNA-mediated integrin-linked kinase suppression: nonspecific effects of siRNA/cationic liposome complexes trigger changes in the expression of phosphorylated-AKT and mTOR independently of ILK silencing. , 2009, Oligonucleotides.

[47]  J. Kingdom,et al.  Glial cell missing-1 transcription factor is required for the differentiation of the human trophoblast , 2009, Cell Death and Differentiation.

[48]  E. Sontheimer,et al.  Origins and Mechanisms of miRNAs and siRNAs , 2009, Cell.

[49]  M. Westwood,et al.  Methods for siRNA-mediated Reduction of mRNA and Protein Expression in Human Placental Explants, Isolated Primary Cells and Cell Lines , 2009, Placenta.

[50]  D. Bartel MicroRNAs: Target Recognition and Regulatory Functions , 2009, Cell.

[51]  S. Hyde,et al.  Progress and Prospects: The design and production of plasmid vectors , 2009, Gene Therapy.

[52]  Hideaki Kashima,et al.  Expression of stathmin, a microtubule regulatory protein, is associated with the migration and differentiation of cultured early trophoblasts. , 2008, Human reproduction.

[53]  P. Baker,et al.  Insulin-like growth factor I and II regulate the life cycle of trophoblast in the developing human placenta. , 2008, American journal of physiology. Cell physiology.

[54]  N. Wake,et al.  Hypoxia inducible factor 1 alpha regulates matrigel-induced endovascular differentiation under normoxia in a human extravillous trophoblast cell line. , 2008, Placenta.

[55]  Xiang Gao,et al.  Nonviral gene delivery: What we know and what is next , 2007, The AAPS Journal.

[56]  J. Guibourdenche,et al.  Human placental development is impaired by abnormal human chorionic gonadotropin signaling in trisomy 21 pregnancies. , 2007, Endocrinology.

[57]  C. Pellegrino,et al.  Efficient transfection of DNA or shRNA vectors into neurons using magnetofection , 2007, Nature Protocols.

[58]  S. Guller,et al.  Role of Hypoxia-Inducible Transcription Factors 1α and 2α in the Regulation of Plasminogen Activator Inhibitor-1 Expression in a Human Trophoblast Cell Line , 2007 .

[59]  M. Kiebler,et al.  High-efficiency transfection of mammalian neurons via nucleofection , 2007, Nature Protocols.

[60]  M. Tremblay,et al.  HIV-1 infection of trophoblasts is independent of gp120/CD4 Interactions but relies on heparan sulfate proteoglycans. , 2007, The Journal of infectious diseases.

[61]  T. Kafri,et al.  Gene delivery by lentivirus vectors , 2007, Molecular biotechnology.

[62]  S. Handwerger,et al.  Mitogen-activated protein kinase activates human placental lactogen-B enhancer by an NF-IL6-dependent pathway , 2000, Endocrine.

[63]  S. Guller,et al.  Role of hypoxia-inducible transcription factors 1alpha and 2alpha in the regulation of plasminogen activator inhibitor-1 expression in a human trophoblast cell line. , 2007, Placenta.

[64]  J. Bertram MATra - Magnet Assisted Transfection: combining nanotechnology and magnetic forces to improve intracellular delivery of nucleic acids. , 2006, Current pharmaceutical biotechnology.

[65]  L. Lim,et al.  Widespread siRNA "off-target" transcript silencing mediated by seed region sequence complementarity. , 2006, RNA.

[66]  L. Lim,et al.  Position-specific chemical modification of siRNAs reduces "off-target" transcript silencing. , 2006, RNA.

[67]  Theresa A. Storm,et al.  Fatality in mice due to oversaturation of cellular microRNA/short hairpin RNA pathways , 2006, Nature.

[68]  Y. Sadovsky,et al.  N-Myc Down-regulated Gene 1 Modulates the Response of Term Human Trophoblasts to Hypoxic Injury* , 2006, Journal of Biological Chemistry.

[69]  C. Mendelson,et al.  Adenoviral-mediated gene delivery to trophoblast cells. , 2006, Methods in molecular medicine.

[70]  D. Szukiewicz,et al.  Histamine stimulates alphav-beta3 integrin expression of the human trophoblast through the H(1) receptor. , 2006, Inflammation research : official journal of the European Histamine Research Society ... [et al.].

[71]  D. Szukiewicz,et al.  Histamine stimulates αv-β3 integrin expression of the human trophoblast through the H1 receptor , 2006, Inflammation Research.

[72]  G. Mor,et al.  A Role for TLRs in the Regulation of Immune Cell Migration by First Trimester Trophoblast Cells1 , 2005, The Journal of Immunology.

[73]  N. Rajewsky,et al.  Silencing of microRNAs in vivo with ‘antagomirs’ , 2005, Nature.

[74]  M. Tremblay,et al.  Rab5 and Rab7, but Not ARF6, Govern the Early Events of HIV-1 Infection in Polarized Human Placental Cells1 , 2005, The Journal of Immunology.

[75]  C. Mendelson,et al.  O2 Enhancement of Human Trophoblast Differentiation and hCYP19 (Aromatase) Gene Expression Are Mediated by Proteasomal Degradation of USF1 and USF2 , 2005, Molecular and Cellular Biology.

[76]  N. Copeland,et al.  Region-specific saturation germline mutagenesis in mice using the Sleeping Beauty transposon system , 2005, Nature Methods.

[77]  R. Shiekhattar,et al.  TRBP recruits the Dicer complex to Ago2 for microRNA processing and gene silencing , 2005, Nature.

[78]  Elisa Izaurralde,et al.  Decay of mRNAs targeted by RISC requires XRN1, the Ski complex, and the exosome. , 2005, RNA.

[79]  A. Vultur,et al.  Transfection techniques affecting Stat3 activity levels. , 2005, Analytical biochemistry.

[80]  S. Akira,et al.  Sequence-specific potent induction of IFN-α by short interfering RNA in plasmacytoid dendritic cells through TLR7 , 2005, Nature Medicine.

[81]  Wei Ge,et al.  Synthetic shRNAs as potent RNAi triggers , 2005, Nature Biotechnology.

[82]  M. Tremblay,et al.  Endocytic Host Cell Machinery Plays a Dominant Role in Intracellular Trafficking of Incoming Human Immunodeficiency Virus Type 1 in Human Placental Trophoblasts , 2004, Journal of Virology.

[83]  N. Bessis,et al.  Immune responses to gene therapy vectors: influence on vector function and effector mechanisms , 2004, Gene therapy.

[84]  J. Milbrandt,et al.  Increased Nuclear NAD Biosynthesis and SIRT1 Activation Prevent Axonal Degeneration , 2004, Science.

[85]  B. Aronow,et al.  Critical role for transcription factor AP-2alpha in human trophoblast differentiation. , 2004, Physiological genomics.

[86]  D. Weissman,et al.  Small Interfering RNAs Mediate Sequence-Independent Gene Suppression and Induce Immune Activation by Signaling through Toll-Like Receptor 31 , 2004, The Journal of Immunology.

[87]  Susana Castro-Obregon,et al.  New non-viral method for gene transfer into primary cells. , 2004, Methods.

[88]  A. Dinda,et al.  Differential expression of CXCR4 receptor in early and term human placenta. , 2004, Placenta.

[89]  C. Delenda Lentiviral vectors: optimization of packaging, transduction and gene expression , 2004, The journal of gene medicine.

[90]  D. Bartel MicroRNAs Genomics, Biogenesis, Mechanism, and Function , 2004, Cell.

[91]  Michael R. Green,et al.  Nonspecific, concentration-dependent stimulation and repression of mammalian gene expression by small interfering RNAs (siRNAs). , 2004, RNA.

[92]  J. Lanner,et al.  Insulin receptor activation and down-regulation by cationic lipid transfection reagents , 2004, BMC Cell Biology.

[93]  M. Sakaguchi,et al.  Lentiviral Vector: A Useful Tool for Transduction of Human Liver Endothelial Cells , 2003, ASAIO journal.

[94]  U. Ikeda,et al.  Efficient gene transfer of a simian immuno‐deficiency viral vector into cardiomyocytes derived from primate embryonic stem cells , 2003, The journal of gene medicine.

[95]  D. Palmer,et al.  Improved system for helper-dependent adenoviral vector production. , 2003, Molecular therapy : the journal of the American Society of Gene Therapy.

[96]  C. Mendelson,et al.  USF1 and USF2 Mediate Inhibition of Human Trophoblast Differentiation and CYP19 Gene Expression by Mash-2 and Hypoxia , 2003, Molecular and Cellular Biology.

[97]  P. Hawley-Nelson,et al.  Transfection of Cultured Eukaryotic Cells Using Cationic Lipid Reagents , 2003, Current protocols in cell biology.

[98]  S. Handwerger,et al.  Identification of an enhancer of the human activating protein-2alpha gene that contains a critical Ets1 binding site. , 2003, The Journal of clinical endocrinology and metabolism.

[99]  Thomas Nevian,et al.  High-efficiency transfection of individual neurons using modified electrophysiology techniques , 2003, Journal of Neuroscience Methods.

[100]  B. Li,et al.  Expression profiling reveals off-target gene regulation by RNAi , 2003, Nature Biotechnology.

[101]  S. Fesik,et al.  Specificity of short interfering RNA determined through gene expression signatures , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[102]  Michael T. McManus,et al.  A lentivirus-based system to functionally silence genes in primary mammalian cells, stem cells and transgenic mice by RNA interference , 2003, Nature Genetics.

[103]  Lung-Ji Chang,et al.  Self-inactivating lentiviral vectors and a sensitive Cre-loxP reporter system. , 2003, Methods in molecular medicine.

[104]  G. Bismuth,et al.  Imaging antigen-induced PI3K activation in T cells , 2002, Nature Immunology.

[105]  A. McAllister,et al.  Techniques for gene transfer into neurons , 2002, Current Opinion in Neurobiology.

[106]  T. Rana,et al.  RNAi in human cells: basic structural and functional features of small interfering RNA. , 2002, Molecular cell.

[107]  Á. McKnight,et al.  Cell surface receptors, virus entry and tropism of primate lentiviruses. , 2002, The Journal of general virology.

[108]  Patrick J. Paddison,et al.  Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells. , 2002, Genes & development.

[109]  R. Bernards,et al.  A System for Stable Expression of Short Interfering RNAs in Mammalian Cells , 2002, Science.

[110]  R. Schild,et al.  The Activity of PPAR in Primary Human Trophoblasts Is Enhanced by Oxidized Lipids , 2002 .

[111]  M. Eder,et al.  Gene transfer into hematopoietic stem cells using lentiviral vectors. , 2002, Current gene therapy.

[112]  P. Godowski,et al.  Tissue Expression of Human Toll-Like Receptors and Differential Regulation of Toll-Like Receptor mRNAs in Leukocytes in Response to Microbes, Their Products, and Cytokines , 2002, The Journal of Immunology.

[113]  R. Schild,et al.  The activity of PPAR gamma in primary human trophoblasts is enhanced by oxidized lipids. , 2002, The Journal of clinical endocrinology and metabolism.

[114]  C. Lois,et al.  Retroviruses as tools to study the immune system. , 2001, Current opinion in immunology.

[115]  A. Fire,et al.  Specific inhibition of gene expression by small double-stranded RNAs in invertebrate and vertebrate systems , 2001, Proceedings of the National Academy of Sciences of the United States of America.

[116]  A. Makrigiannakis,et al.  Differential Expression of the Coxsackievirus and Adenovirus Receptor Regulates Adenovirus Infection of the Placenta1 , 2001, Biology of reproduction.

[117]  R. Ulevitch,et al.  Cloning and characterization of a sub-family of human toll-like receptors: hTLR7, hTLR8 and hTLR9. , 2000, European cytokine network.

[118]  Y. Sadovsky,et al.  Peroxisome proliferator-activated receptor-γ modulates differentiation of human trophoblast in a ligand-specific manner , 2000 .

[119]  C. Mendelson,et al.  Hypoxia prevents induction of aromatase expression in human trophoblast cells in culture: potential inhibitory role of the hypoxia-inducible transcription factor Mash-2 (mammalian achaete-scute homologous protein-2). , 2000, Molecular endocrinology.

[120]  P. Roques,et al.  HIV‐1 co‐receptor expression on trophoblastic cells from early placentas and permissivity to infection by several HIV‐1 primary isolates , 2000, Clinical and experimental immunology.

[121]  C. Kessler,et al.  Activator protein-2 regulates human placental lactogen gene expression , 2000, Molecular and Cellular Endocrinology.

[122]  V. Goiffon,et al.  Calcium phosphate transfection of mammalian cultured cells. , 2000, Methods in molecular biology.

[123]  S. Smith,et al.  The Journal of Clinical Endocrinology & Metabolism Printed in U.S.A. Copyright © 2000 by The Endocrine Society Peroxisome Proliferator-Activated Receptor- � Modulates Differentiation of Human Trophoblast in a Ligand-Specific Manner* , 2022 .

[124]  R. Lehmann,et al.  Targeted mRNA degradation by double-stranded RNA in vitro. , 1999, Genes & development.

[125]  J. Bloch,et al.  Lentiviral Gene Transfer to the Nonhuman Primate Brain , 1999, Experimental Neurology.

[126]  C. Kaltschmidt,et al.  Optimized protocol for biolistic transfection of brain slices and dissociated cultured neurons with a hand-held gene gun , 1999, Journal of Neuroscience Methods.

[127]  A. Cheung,et al.  Apoptotic and proliferative activities in first trimester placentae. , 1999, Placenta.

[128]  A. Fire,et al.  Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans , 1998, Nature.

[129]  R. Plasterk,et al.  Molecular Reconstruction of Sleeping Beauty , a Tc1-like Transposon from Fish, and Its Transposition in Human Cells , 1997, Cell.

[130]  Y. Sadovsky,et al.  Transcriptional regulation of prostaglandin-H synthase-2 gene in human trophoblasts. , 1997, The Journal of clinical endocrinology and metabolism.

[131]  J. Bergelson,et al.  Isolation of a Common Receptor for Coxsackie B Viruses and Adenoviruses 2 and 5 , 1997, Science.

[132]  G. Nemerow,et al.  Integrin alpha v beta 5 selectively promotes adenovirus mediated cell membrane permeabilization , 1994, The Journal of cell biology.

[133]  R. Kumar,et al.  Enhanced gene delivery and mechanism studies with a novel series of cationic lipid formulations. , 1994, The Journal of biological chemistry.

[134]  G. Nemerow,et al.  Integrins α v β 3 and α v β 5 promote adenovirus internalization but not virus attachment , 1993, Cell.

[135]  J. Chermann,et al.  Human transformed trophoblast-derived cells lacking CD4 receptor exhibit restricted permissiveness for human immunodeficiency virus type 1 , 1991, Journal of virology.

[136]  H. Fox,et al.  Ultrastructure of the normal human placenta. , 1991, Electron microscopy reviews.

[137]  D. Cheresh,et al.  Purification and functional characterization of integrin alpha v beta 5. An adhesion receptor for vitronectin. , 1990, The Journal of biological chemistry.

[138]  J. Northrop,et al.  Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure. , 1987, Proceedings of the National Academy of Sciences of the United States of America.

[139]  J. Alwine Transient gene expression control: effects of transfected DNA stability and trans-activation by viral early proteins , 1985, Molecular and cellular biology.

[140]  S. Bacchetti,et al.  Transfer of the gene for thymidine kinase to thymidine kinase-deficient human cells by purified herpes simplex viral DNA. , 1977, Proceedings of the National Academy of Sciences of the United States of America.