Metabolic interactions between animal cells through permeable intercellular junctions.

Abstract In mixed cultures of HGPRT− (lacking hypoxanthine: guanine phosphoribosyltransferase activity) and wild-type mammalian cells coupled by permeable intercellular junctions, the purine nucleotide pools equilibrate between the two cell types prior to incorporation into nucleic acid. As a consequence, in the presence of exogenous hypoxanthine, the mutant cells stimulate the activity of HGPRT pathway in the wild-type cells and the de novo pathway of purine nucleotide biosynthesis is inhibited in both cell types. That is, the metabolic activity of a mixed culture of coupled cells is a unique characteristic of the mixture, not simply the sum of the activities of the component cells, whereas the activity of a mixed culture of cells which are not coupled by permeable intercellular junctions is simply the sum of the activities of the two cell types.

[1]  J D Pitts,et al.  Metabolic Co-operation between Biochemically Marked Mammalian Cells in Tissue Culture , 2022 .

[2]  J. Subak-Sharpe,et al.  Exchange between hamster cells in culture. , 1968, Experimental cell research.

[3]  A W Murray,et al.  The biological significance of purine salvage. , 1971, Annual Review of Biochemistry.

[4]  D. Potter,et al.  Chapter 4 Low-Resistance Junctions Between Cells in Embryos and Tissue Culture , 1968 .

[5]  J. Sheridan.  Functional Evaluation of Low Resistance Junctions: Influence of Cell Shape and Size , 1973 .

[6]  P. Plagemann,et al.  Transport of nucleosides, nucleic acid bases, choline and glucose by animal cells in culture. , 1974, Biochimica et biophysica acta.

[7]  R. Dehaan,et al.  Cell coupling in developing systems: the heart-cell paradigm. , 1972, Current topics in developmental biology.

[8]  W. Loewenstein,et al.  PERMEABILITY OF MEMBRANE JUNCTIONS * , 1966, Annals of the New York Academy of Sciences.

[9]  J. Pitts,et al.  Permeability of junctions between animal cells. Intercellular transfer of nucleotides but not of macromolecules. , 1977, Experimental cell research.

[10]  Henderson Jf,et al.  AVAILABILITY OF 5-PHOSPHORIBOSYL 1-PYROPHOSPHATE FOR RIBONUCLEOTIDE SYNTHESIS IN EHRLICH ASCITES TUMOR CELLS IN VITRO. , 1965 .

[11]  J. Littlefield,et al.  Selection of Morphologically Normal Cell Lines from Polyoma-transformed BHK21/13 Hamster Fibroblasts , 1968, Journal of Virology.

[12]  K. Sanford,et al.  The growth in vitro of single isolated tissue cells. , 1948, Journal of the National Cancer Institute.

[13]  N. Gilula,et al.  Metabolic Coupling, Ionic Coupling and Cell Contacts , 1972, Nature.

[14]  J. Littlefield.  The use of drug-resistant markers to study the hybridization of mouse fibroblasts. , 1966, Experimental cell research.

[15]  I. Macpherson,et al.  Polyoma transformation of hamster cell clones--an investigation of genetic factors affecting cell competence. , 1962, Virology.