A versatile time-sharing multichannel spectrophotometer, reflectometer, and fluorometer.

A simple turbine-driven multi-wavelength time-sharing apparatus affords flexibility and versatility in optical measurements and serves as a fluorometer, reflectometer, and spectrophotometer for four wavelength channels. As a reflectance fluorometer, nearly simultaneous readout of tissue reflectance and NADH or flavoprotein fluorescence can be obtained. As a transmission spectrophotometer and reflectometer, hemoproteins such as myoglobin, catalase, and cytochrome a + a3 with its copper component may be measured. This instrument offers the advantages of high speed of time-sharing, and simplicity, compactness, and flexibility not found in previous designs. In addition, it causes minimal acoustic and electrical disturbance to the experimental system.

[1]  F. Jöbsis,et al.  Intracellular redox changes in functioning cerebral cortex. I. Metabolic effects of epileptiform activity. , 1971, Journal of neurophysiology.

[2]  M. Baltscheffsky Inorganic Pyrophosphate and ATP as Energy Donors in Chromatophores from Rhodospirillum rubrum , 1967, Nature.

[3]  B. Chance,et al.  Kinetics of mitochondrial flavoprotein and pyridine nucleotide in perfused heart , 1972 .

[4]  V. Legallais,et al.  Rapid Readout from Dual Wavelength Spectrophotometers , 1972 .

[5]  B. Chance,et al.  Intracellular Oxidation-Reduction States in Vivo , 1962, Science.

[6]  V. Legallais,et al.  Compensation for Light Source Noise in a Sensitive Dual Wavelength Spectrophotometer , 1970 .

[7]  B. Chance,et al.  A time sharing fluorometer for the readout of intracellular oxidation-reduction states of NADH and flavoprotein. , 1971, The Review of scientific instruments.

[8]  E. Ogata,et al.  Microfluorometry of oxidation-reduction state of the rat kidney in situ. , 1971, Journal of applied physiology.

[9]  V. Legallais,et al.  Combined Fluorometer and Double‐Beam Spectrophotometer for Reflectance Measurements , 1963 .

[10]  B. Chance,et al.  Flavin and pyridine nucleotide oxidation-reduction changes in perfused rat liver. I. Anoxia and subcellular localization of fluorescent flavoproteins. , 1969, The Journal of biological chemistry.

[11]  H. Sies,et al.  State of oxidation-reduction and state of binding in the cytosolic NADH-system as disclosed by equilibration with extracellular lactate-pyruvate in hemoglobin-free perfused rat liver. , 1972, European journal of biochemistry.