Rapid endosomal escape of prickly nanodiamonds: implications for gene delivery

The prickly nanodiamonds easily entered cells via endocytosis followed by unique intracellular translocation characteristics—quick endosomal escape followed by stable residence in cytoplasm. Endosomal membrane rupturing is identified as the major route of nanodiamonds’ escaping the vesicle confinement and to the cytoplasm. Little cytotoxicity is observed to associate with the nanodiamonds’ cytosolic release. Such features enable its application for gene delivery, which requires both effective cellular uptake and cytosolic release of the gene. Taking green fluorescent protein gene as an example, we demonstrate the successful cytosolic delivery and expression of such a gene using the prickly nanodiamonds as carrier.

[1]  R. Mahato.  Water insoluble and soluble lipids for gene delivery. , 2005, Advanced drug delivery reviews.

[2]  Daniel W. Pack,et al.  Design and development of polymers for gene delivery , 2005, Nature Reviews Drug Discovery.

[3]  Huan-Cheng Chang,et al.  The biocompatibility of fluorescent nanodiamonds and their mechanism of cellular uptake , 2009, Nanotechnology.

[4]  Jin-Zhi Du,et al.  Gold nanoparticles capped with polyethyleneimine for enhanced siRNA delivery. , 2010, Small.

[5]  Shubiao Zhang,et al.  Toxicity of cationic lipids and cationic polymers in gene delivery. , 2006, Journal of controlled release : official journal of the Controlled Release Society.

[6]  Huan-Cheng Chang,et al.  In vivo imaging and toxicity assessments of fluorescent nanodiamonds in Caenorhabditis elegans. , 2010, Nano letters.

[7]  Huan-Cheng Chang,et al.  Mass production and dynamic imaging of fluorescent nanodiamonds. , 2008, Nature nanotechnology.

[8]  A. Prescott,et al.  The coated pit and macropinocytic pathways serve distinct endosome populations , 1994, The Journal of cell biology.

[9]  Morteza Mahmoudi,et al.  Protein-Nanoparticle Interactions , 2013 .

[10]  Q. Tao,et al.  Cellular uptake, evolution, and excretion of silica nanoparticles in human cells. , 2011, Nanoscale.

[11]  S. Aaronson,et al.  Implications for Cancer Therapy , 2003 .

[12]  P. Maurer,et al.  Nanometre-scale thermometry in a living cell , 2013, Nature.

[13]  Y. Liu,et al.  Selective targeting of gold nanorods at the mitochondria of cancer cells: implications for cancer therapy. , 2011, Nano letters.

[14]  Courtney R. Thomas,et al.  Involvement of lysosomal exocytosis in the excretion of mesoporous silica nanoparticles and enhancement of the drug delivery effect by exocytosis inhibition. , 2013, Small.

[15]  Kenneth A. Dawson,et al.  Protein–Nanoparticle Interactions , 2008, Nano-Enabled Medical Applications.

[16]  Marco P Monopoli,et al.  Biomolecular coronas provide the biological identity of nanosized materials. , 2012, Nature nanotechnology.

[17]  Yury Gogotsi,et al.  Adsorption of drugs on nanodiamond: toward development of a drug delivery platform. , 2013, Molecular pharmaceutics.

[18]  Acknowledgements , 1992, Experimental Gerontology.

[19]  Quan Li,et al.  Free Energy Calculation of Nanodiamond-Membrane Association-The Effect of Shape and Surface Functionalization. , 2014, Journal of chemical theory and computation.

[20]  Christof von Kalle,et al.  A serious adverse event after successful gene therapy for X-linked severe combined immunodeficiency. , 2003, The New England journal of medicine.

[21]  G. Lin,et al.  Controllable drug release and simultaneously carrier decomposition of SiO2-drug composite nanoparticles. , 2013, Journal of the American Chemical Society.

[22]  S Moein Moghimi,et al.  A two-stage poly(ethylenimine)-mediated cytotoxicity: implications for gene transfer/therapy. , 2005, Molecular therapy : the journal of the American Society of Gene Therapy.

[23]  Huan-Cheng Chang,et al.  Tracking the Engraftment and Regenerative Capabilities of Transplanted Lung Stem Cells using Fluorescent Nanodiamonds , 2014 .

[24]  T. Xia,et al.  Understanding biophysicochemical interactions at the nano-bio interface. , 2009, Nature materials.

[25]  K. Kinzler,et al.  A nanoparticle formulation that selectively transfects metastatic tumors in mice , 2013, Proceedings of the National Academy of Sciences.

[26]  T. Bein,et al.  Role of endosomal escape for disulfide-based drug delivery from colloidal mesoporous silica evaluated by live-cell imaging. , 2010, Nano letters.

[27]  V. S. Lin,et al.  Mesoporous silica nanoparticles deliver DNA and chemicals into plants. , 2007, Nature nanotechnology.

[28]  J Wrachtrup,et al.  Magnetic spin imaging under ambient conditions with sub-cellular resolution. , 2013, Nature communications.

[29]  Luigi Naldini,et al.  Viral vectors for gene therapy: the art of turning infectious agents into vehicles of therapeutics , 2001, Nature Medicine.

[30]  Antonios Kontsos,et al.  Mechanical properties and biomineralization of multifunctional nanodiamond-PLLA composites for bone tissue engineering. , 2012, Biomaterials.

[31]  Ge Lin,et al.  Unambiguous observation of shape effects on cellular fate of nanoparticles , 2014, Scientific Reports.

[32]  Q. Tao,et al.  Physiological pathway of human cell damage induced by genotoxic crystalline silica nanoparticles. , 2012, Biomaterials.

[33]  A. Goga,et al.  Nanodiamond Therapeutic Delivery Agents Mediate Enhanced Chemoresistant Tumor Treatment , 2011, Science Translational Medicine.

[34]  Jayanth Panyam,et al.  Biodegradable nanoparticles for drug and gene delivery to cells and tissue. , 2003, Advanced drug delivery reviews.

[35]  Ru Bai,et al.  Surface chemistry of gold nanorods: origin of cell membrane damage and cytotoxicity. , 2013, Nanoscale.

[36]  Yury Gogotsi,et al.  Fluorescent PLLA-nanodiamond composites for bone tissue engineering. , 2011, Biomaterials.

[37]  J. Benoit,et al.  Progress in developing cationic vectors for non-viral systemic gene therapy against cancer. , 2008, Biomaterials.

[38]  S. Schmid,et al.  ATP is required for receptor-mediated endocytosis in intact cells , 1990, The Journal of cell biology.

[39]  G. Kroemer,et al.  Lysosomal membrane permeabilization in cell death , 2008, Oncogene.