LASER PHOTOLYSIS OF 3‐METHYLLUMIFLAVIN

Abstract—The pH dependence of the transient absorptions of 3‐methyllumiflavin has been investigated with laser photolysis in the nanosecond time range. Three different transient species (I, II and III) can be observed. The analysis of the decay curves shows that I and II can be ascribed to the optical absorption spectra of the lowest excited singlet and triplet states, respectively. Their appearance is not affected by a change of pH of the solution. The transient spectra due to species III have a decay which is dependent on pH. By comparison of these results with spectral data obtained with flavin dissolved in polymethylmethacrylate and with photochemical data reported in the literature, a structure for III is proposed which might explain the observed pH dependence.

[1]  D. Bebelaar.  Time resolved molecular spectroscopy using high power solid state lasers in pulse transmission mode. A re-examination of the Sn← S1 spectra of naphthalene and anthracene , 1974 .

[2]  V. Massey,et al.  On the effect of temperature on the absorption spectra of free and protein-bound flavines. , 1973, Biochemistry.

[3]  T. Moore,et al.  Molecular Luminescence Studies of Flavins, II. , 1972, Zeitschrift fur Naturforschung. Teil B. Anorganische Chemie, organische Chemie, Biochemie, Biophysik, Biologie.

[4]  T. Moore,et al.  Molecular luminescence studies of flavins. I. The excited states of flavins. , 1972, Journal of the American Chemical Society.

[5]  P. Hemmerich,et al.  pH-Dependence, Isotope Effect and Products of Flavin-sensitized Photodecarboxylation and Photodehydrogenation , 1972, Zeitschrift fur Naturforschung. Teil B. Anorganische Chemie, organische Chemie, Biochemie, Biophysik, Biologie.

[6]  P. Hemmerich,et al.  Neue Photo‐Alkylierungsreaktionen am Flavin‐Kern , 1971 .

[7]  J. Langelaar,et al.  Singlet excited monomer and excimer absorptions of pyrene , 1971 .

[8]  W. Pfleiderer,et al.  Pteridine, XLIV. Über die Synthese und Struktur N‐8‐substituierter Pterine und Lumazine , 1971 .

[9]  M. B. Katan,et al.  pH dependence of the transient absorptions in the flash photolysis of 3-methyllumiflavin. , 1971, Biochimica et biophysica acta.

[10]  J. Langelaar,et al.  Excited singlet state polarization and absorption spectra of 1,2-benzcoronene, 1,12-benzperylene and 1,2:3,4-dibenzanthracene , 1971 .

[11]  G. Tollin,et al.  Flash photolysis of flavins. IV. Some properties of the lumiflavin triplet state , 1970 .

[12]  G. Radda,et al.  Chemical properties of flavins in relation to flavoprotein catalysis. , 1970, Vitamins and hormones.

[13]  J. Langelaar,et al.  Sensitivity improvements in spectrophospho-fluorimetry. , 1969, Journal of scientific instruments.

[14]  N. Kaplan,et al.  Investigations of inter- and intramolecular interations in flavin--adenine dinucleotide by proton magnetic resonance. , 1968, Biochemistry.

[15]  A. Knowles,et al.  A FLASH‐PHOTOLYSIS INVESTIGATION OF FLAVIN PHOTOSENSITIZATION OF PURINE NUCLEOTIDES , 1968, Photochemistry and photobiology.

[16]  P. Hemmerich,et al.  Basicity, visible spectra, and electron spin resonance of flavosemiquinone anions. , 1967, European journal of biochemistry.

[17]  O. Jardetzky,et al.  An Experimental Demonstration of the Nuclear Magnetic Resonance Assignments in the 6,7-Dimethylisoalloxazine Nucleus* , 1965 .

[18]  H. Erlenmeyer,et al.  Studien in der Lumiflavin‐Reihe V. Spezifische Reaktivität 8‐ständiger Substituenten am Isoalloxazin‐Kern Flavin‐Dimere , 1959 .

[19]  H. Erlenmeyer,et al.  Synthesen in der Lumiflavinreihe IV , 1956 .