Comparison of Major Compounds in Illicii Veri Fructus by Extraction Solvents

ABSTRACT Objectives : The Illicii Veri Fructus was not only traditional medicine but also food in Asia. The aim of this study was selection of optimum solvent in the fruit of Illicii Veri Fructus because an appropriate solvent affect a medicinal effect.Methods : Illicii Veri Fructus was carried out ultrasonic-assisted extra ction as various solvents. Two main compounds, p-anisaldehyde and anethole, were successfully analyzed by high performance liquid chromatography-photodiode array detector (HPLC-PDA) and carried out method validation according to ICH guideline. The optimum solvent selected by comparing with yields of two main ingredients.Results : The p-anisaldehyde and anethole were detected at approximately 8.0 min and 19.8 min, respectively. It was all below 5.0% that RSD of retention time and peak area for two main peaks. Calibration curves of two compounds were good linearity as R 2 >0.9999. All of the precisions and accuracy were good intra-day and inter-day as below 5.0% RSD. Limited of detection (LOD) of p-anisaldehyde and anethole were analyzed as 0.134 µg/mL and 4.286 µg/mL, respectively. Limited of quantification (LOQ) of two comp ounds were 0.407 µg/mL and 12.989 µg/mL, respectively. As a result of this study, p-anisladehyde was detected as 0.209 ~ 0.467%, however anethole was not detected in the distilled wate r.Conclusions : Anethole was main component as 5.329 ~ 6.815% except for water extraction. Methanol extraction among various solvents was detected the highest cont ents of p-anisaldehyde and anethole as 0.467(±0.008)% and 6.815(±0.220)%, respectively. Key words : Illicii Veri Fructus, anethole, p-anisaldehyde, HPLC-PDA