Correlation between sperm characteristics and testosterone in bovine seminal plasma by direct radioimmunoassay

The objectives of this study were to validate a non-extractive RIA for seminal testosterone and quantify the hormone using a solid-phase commercial kit, and study the correlation between testosterone in seminal plasma and sperm characteristics. Parallelism showed a correlation index r = 0.992 (Y = -5.47 + 1.073X; R2 = 0.985), indicating that the non-extractive method presented is indicated particularly for assessment of testosterone when establishing comparisons between samples. Overall mean (±SD) of testosterone level was 0.60±0.65 ng/mL. Correlation was only found between the seminal concentrations of testosterone and pH of the semen.

[1]  S. Asakuma,et al.  Changes of fructose concentrations in seminal plasma and glucose and testosterone concentrations in blood plasma in rams over the course of a year. , 2006, The Journal of reproduction and development.

[2]  J. Ruas,et al.  Potencial reprodutivo de touros da raça Nelore submetidos a diferentes proporções touro: vaca , 2004 .

[3]  B. Breier,et al.  Growth hormone treatment of breeding bulls used for artificial insemination improves fertilization rates. , 2000, Domestic animal endocrinology.

[4]  J. Guéchot,et al.  Seminal androgen concentrations and residual sperm cytoplasm. , 1998, Clinica chimica acta; international journal of clinical chemistry.

[5]  A. Amador,et al.  Effects of somatostatin and somatotropin on the in vitro testicular steroidogenesis in hamster. , 1995, Revista espanola de fisiologia.

[6]  M. Andersson.  Relationships between GnRH-induced testosterone maxima, sperm motility and fertility in Ayrshire bulls , 1992 .

[7]  D. Schams,et al.  Divergent Effects of Estrogens on the Somatotropic Axis in Male and Female Calves , 1992 .

[8]  K. Borg,et al.  Cortisol, growth hormone, and testosterone concentrations during mating behavior in the bull and boar. , 1991, Journal of animal science.

[9]  G. Aumüller,et al.  Protein secretion and secretory processes in male accessory sex glands. , 1990, International review of cytology.

[10]  D. Robertson,et al.  The effects of exogenously administered testosterone on spermatogenesis in intact and hypophysectomized rats. , 1989, Endocrinology.

[11]  B. Schanbacher,et al.  Validation of a Direct Radioimmunoassay for Testosterone in Unextracted Serum from Five Species: Application to Study of the Hypothalamic- Pituitary-Gonadal Axis in Males , 1982 .

[12]  T. Mann,et al.  Biochemistry of Spermatozoa: Chemical and Functional Correlations in Ejaculated Semen, Andrological Aspect , 1981 .

[13]  T. Mann,et al.  Male reproductive function and semen. , 1981 .

[14]  T. Post,et al.  Testosterone variability and fertility in bulls , 1976 .