SERS nanoprobes for the monitoring of endogenous nitric oxide in living cells.

Nitric Oxide (NO) is a significant gaseous signalling molecule in various pathological and physiological pathways, whereas many of its functions are still ambiguous in part because of the shortage of powerful detection approaches. Herein, we present a type of reaction-based surface-enhanced Raman scattering (SERS) nanoprobes, o-phenylenediamine-modified gold nanoparticles (AuNPs/OPD), to detect the level of the endogenous NO in living cells. The detection is achieved through the SERS variation of AuNPs/OPD caused by the reaction between NO and OPD on the surface of AuNPs. The proposed SERS nanoprobes have a good stability and a rapid response to NO within 30s Moreover, as a result of the reaction specificity coupled with SERS fingerprinting, AuNPs/OPD nanoprobes demonstrate high selectivity towards NO over other biologically relevant species with a sensitivity at 10(-7)M level. Thereby, this SERS strategy can be used for monitoring NO that is endogenously produced in living macrophages, indicating immense potential in studying NO-involved pathophysiological processes in biological systems.

[1]  C. Dessy,et al.  Rational design of a fluorescent NADPH derivative imaging constitutive nitric-oxide synthases upon two-photon excitation , 2012, Proceedings of the National Academy of Sciences.

[2]  Simon C Watkins,et al.  Inducible nitric oxide synthase suppresses the development of allograft arteriosclerosis. , 1997, The Journal of clinical investigation.

[3]  L. Boscá,et al.  Nitric oxide and cell viability in inflammatory cells: a role for NO in macrophage function and fate. , 2005, Toxicology.

[4]  R. Aebersold,et al.  Identification of Flow-dependent Endothelial Nitric-oxide Synthase Phosphorylation Sites by Mass Spectrometry and Regulation of Phosphorylation and Nitric Oxide Production by the Phosphatidylinositol 3-Kinase Inhibitor LY294002* , 1999, The Journal of Biological Chemistry.

[5]  A. Nel,et al.  Real-time electrical detection of nitric oxide in biological systems with sub-nanomolar sensitivity , 2013, Nature Communications.

[6]  Bing Yan,et al.  SERS tags: novel optical nanoprobes for bioanalysis. , 2013, Chemical reviews.

[7]  Weiming Xu,et al.  The role of nitric oxide in cancer , 2002, Cell Research.

[8]  Barry Halliwell,et al.  Formation of nitric oxide-derived inflammatory oxidants by myeloperoxidase in neutrophils , 1998, Nature.

[9]  H. Tian,et al.  Monitoring of Endogenous Hydrogen Sulfide in Living Cells Using Surface-Enhanced Raman Scattering. , 2015, Angewandte Chemie.

[10]  S. Snyder,et al.  Nitric oxide: a physiologic messenger molecule. , 1994, Annual review of biochemistry.

[11]  A. Wan,et al.  Fluorescent probes for real-time measurement of nitric oxide in living cells. , 2015, The Analyst.

[12]  S. Lippard,et al.  Visualization of nitric oxide in living cells by a copper-based fluorescent probe , 2006, Nature chemical biology.

[13]  Sei-Jung Lee,et al.  Phytoglycoprotein inhibits interleukin-1β and interleukin-6 via p38 mitogen-activated protein kinase in lipopolysaccharide-stimulated RAW 264.7 cells , 2008, Naunyn-Schmiedeberg's Archives of Pharmacology.

[14]  D. Meisel,et al.  Adsorption and surface-enhanced Raman of dyes on silver and gold sols , 1982 .

[15]  A. Friebe,et al.  Fatal gastrointestinal obstruction and hypertension in mice lacking nitric oxide-sensitive guanylyl cyclase , 2007, Proceedings of the National Academy of Sciences.

[16]  A. Facchini,et al.  Differential roles of nitric oxide and oxygen radicals in chondrocytes affected by osteoarthritis and rheumatoid arthritis. , 2001, Clinical science.

[17]  H. Schaefer,et al.  The photohydration of N-alkylpyridinium salts: theory and experiment. , 2001, Chemistry.

[18]  C. Harris,et al.  The chemical biology of nitric oxide: implications in cellular signaling. , 2008, Free radical biology & medicine.

[19]  D. Wink,et al.  A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite. , 2001, Nitric oxide : biology and chemistry.

[20]  I Rovira,et al.  Nitric oxide , 2021, Reactions Weekly.

[21]  Sebastian Schlücker,et al.  Label-free SERS monitoring of chemical reactions catalyzed by small gold nanoparticles using 3D plasmonic superstructures. , 2013, Journal of the American Chemical Society.

[22]  Sanjiv S. Gambhir,et al.  Multiplexed imaging of surface enhanced Raman scattering nanotags in living mice using noninvasive Raman spectroscopy , 2009, Proceedings of the National Academy of Sciences.

[23]  M. Moskowitz,et al.  Stroke protection by 3-hydroxy-3-methylglutaryl (HMG)-CoA reductase inhibitors mediated by endothelial nitric oxide synthase. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[24]  M. Recanatini,et al.  Revealing DNA interactions with exogenous agents by surface-enhanced Raman scattering. , 2015, Journal of the American Chemical Society.

[25]  Weihua Huang,et al.  Functionalized graphene-based biomimetic microsensor interfacing with living cells to sensitively monitor nitric oxide release , 2015, Chemical science.

[26]  Yasuteru Urano,et al.  Highly sensitive fluorescence probes for nitric oxide based on boron dipyrromethene chromophore-rational design of potentially useful bioimaging fluorescence probe. , 2004, Journal of the American Chemical Society.

[27]  Steven R. Emory,et al.  Probing Single Molecules and Single Nanoparticles by Surface-Enhanced Raman Scattering , 1997, Science.

[28]  Stephen E. Flower,et al.  A water-soluble boronate-based fluorescent probe for the selective detection of peroxynitrite and imaging in living cells† , 2014 .

[29]  Luca Guerrini,et al.  Highly sensitive SERS quantification of the oncogenic protein c-Jun in cellular extracts. , 2013, Journal of the American Chemical Society.

[30]  Evan W. Miller,et al.  Fluorescent probes for nitric oxide and hydrogen peroxide in cell signaling. , 2007, Current opinion in chemical biology.

[31]  C. Nathan,et al.  Nitric oxide as a secretory product of mammalian cells , 1992, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[32]  R. Aumann,et al.  "Dimers" and "trimers" of tetrahydroindenes and hexahydroazulenes, respectively generated from [2-(1-cycloalkenyl)ethynyl]carbene complexes (M = W, Cr) by cascade cyclization/cycloaddition reactions. , 2001, Chemistry.

[33]  E. Manders,et al.  Controlled light-exposure microscopy reduces photobleaching and phototoxicity in fluorescence live-cell imaging , 2007, Nature Biotechnology.

[34]  M. Marletta,et al.  Mammalian nitrate biosynthesis: mouse macrophages produce nitrite and nitrate in response to Escherichia coli lipopolysaccharide. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[35]  R. Frontiera,et al.  SERS: Materials, applications, and the future , 2012 .

[36]  M. El-Sayed,et al.  Biological Targeting of Plasmonic Nanoparticles Improves Cellular Imaging via the Enhanced Scattering in the Aggregates Formed , 2014, The journal of physical chemistry letters.

[37]  W. Hellstrom,et al.  RhoA/Rho-kinase suppresses endothelial nitric oxide synthase in the penis: A mechanism for diabetes-associated erectile dysfunction , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[38]  Xiaoping Liu,et al.  Accelerated reaction of nitric oxide with O2 within the hydrophobic interior of biological membranes. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[39]  Y. Chu,et al.  Endogenous production and exogenous exposure to nitric oxide augment doxorubicin cytotoxicity for breast cancer cells but not cardiac myoblasts. , 2004, Nitric oxide : biology and chemistry.

[40]  Shuming Nie,et al.  Efficient Raman enhancement and intermittent light emission observed in single gold nanocrystals , 1999 .