Nasal chondrocytes and fibrin sealant for cartilage tissue engineering.

Hybrid constructs associating a biodegradable matrix and autologous chondrocytes hold promise for the treatment of articular cartilage defects. In this context, our objective was to investigate the potential use of nasal chondrocytes associated with a fibrin sealant for the treatment of articular cartilage defects. The phenotype of primary nasal chondrocytes (NC) from human (HNC) and rabbit (RNC) origin were characterized by RT-PCR. The ability of constructs associating fibrin sealant and NC to form a cartilaginous tissue in vivo was investigated, firstly in a subcutaneous site in nude mice and secondly in an articular cartilage defect in rabbit. HNC express type II collagen and aggrecan, the two major hallmarks of a chondrocytic phenotype. Furthermore, when injected subcutaneously into nude mice within a fibrin sealant, these chondrocytes were able to form a cartilage-like tissue. Our data indicate that RNC also express type II collagen and aggrecan and maintained their phenotype in three-dimensional culture within a fibrin sealant. Moreover, treatment of rabbit articular cartilage defects with autologous RNC embedded in a fibrin sealant led to the formation of a hyalin-like repair tissue. The use of fibrin sealant containing hybrid autologous NC therefore appears as a promising approach for cell-based therapy of articular cartilage.

[1]  J. Jansen,et al.  Degradable hydrogel scaffolds for in vivo delivery of single and dual growth factors in cartilage repair. , 2007, Osteoarthritis and cartilage.

[2]  G. Daculsi,et al.  Engineering cartilage with human nasal chondrocytes and a silanized hydroxypropyl methylcellulose hydrogel. , 2007, Journal of biomedical materials research. Part A.

[3]  Y. Chai,et al.  Recent advances in craniofacial morphogenesis , 2006, Developmental dynamics : an official publication of the American Association of Anatomists.

[4]  J. Hardillo,et al.  Short-Duration Enzymatic Treatment Promotes Integration of a Cartilage Graft in a Defect , 2006, The Annals of otology, rhinology, and laryngology.

[5]  Yoshito Ikada,et al.  Comparison of different chondrocytes for use in tissue engineering of cartilage model structures. , 2006, Tissue engineering.

[6]  Dietmar W Hutmacher,et al.  Evaluation of a hybrid scaffold/cell construct in repair of high-load-bearing osteochondral defects in rabbits. , 2006, Biomaterials.

[7]  Vilmos Vécsei,et al.  Cartilage repair: generations of autologous chondrocyte transplantation. , 2006, European journal of radiology.

[8]  Miya Ishihara,et al.  Tissue engineering of articular cartilage using an allograft of cultured chondrocytes in a membrane-sealed atelocollagen honeycomb-shaped scaffold (ACHMS scaffold). , 2005, Journal of biomedical materials research. Part B, Applied biomaterials.

[9]  Feza Korkusuz,et al.  Tissue engineered cartilage on collagen and PHBV matrices. , 2005, Biomaterials.

[10]  K. Athanasiou,et al.  Rapid phenotypic changes in passaged articular chondrocyte subpopulations , 2005, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[11]  E. Kastenbauer,et al.  Tissue engineering of autologous cartilage grafts in three-dimensional in vitro macroaggregate culture system. , 2004, Tissue engineering.

[12]  J. Elisseeff,et al.  Injectable cartilage tissue engineering , 2004, Expert opinion on biological therapy.

[13]  Andrés J. García,et al.  Chondrocyte phenotypes on different extracellular matrix monolayers. , 2004, Biomaterials.

[14]  S. Waldman,et al.  Long-term intermittent compressive stimulation improves the composition and mechanical properties of tissue-engineered cartilage. , 2004, Tissue engineering.

[15]  H. Weinans,et al.  Improved cartilage integration and interfacial strength after enzymatic treatment in a cartilage transplantation model , 2004, Arthritis research & therapy.

[16]  G G Reinholz,et al.  Animal models for cartilage reconstruction. , 2004, Biomaterials.

[17]  M. Martina,et al.  Culture and differentiation of chondrocytes entrapped in alginate gels , 2004, Calcified Tissue International.

[18]  M. Brittberg,et al.  Cartilage repair with chondrocytes: clinical and cellular aspects. , 2003, Novartis Foundation symposium.

[19]  J Malda,et al.  Expansion of human nasal chondrocytes on macroporous microcarriers enhances redifferentiation. , 2003, Biomaterials.

[20]  N. Adachi,et al.  Optimum combination of monolayer and three-dimensional cultures for cartilage-like tissue engineering. , 2003, Tissue engineering.

[21]  Ivan Martin,et al.  Three-dimensional tissue engineering of hyaline cartilage: comparison of adult nasal and articular chondrocytes. , 2002, Tissue engineering.

[22]  J. Mansour,et al.  Immunochemical and Mechanical Characterization of Cartilage Subtypes in Rabbit , 2002, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[23]  C. Gabay,et al.  Primary human articular chondrocytes, dedifferentiated chondrocytes, and synoviocytes exhibit differential responsiveness to interleukin‐4: Correlation with the expression pattern of the common receptor gamma chain , 2002, Journal of cellular physiology.

[24]  E B Hunziker,et al.  Articular cartilage repair: basic science and clinical progress. A review of the current status and prospects. , 2002, Osteoarthritis and cartilage.

[25]  Michael Sittinger,et al.  A tissue-engineering model for the manufacture of auricular-shaped cartilage implants , 2002, European Archives of Oto-Rhino-Laryngology.

[26]  E. Wagner,et al.  Reaching a genetic and molecular understanding of skeletal development. , 2002, Developmental cell.

[27]  V Vécsei,et al.  Dedifferentiation-associated changes in morphology and gene expression in primary human articular chondrocytes in cell culture. , 2002, Osteoarthritis and cartilage.

[28]  K. Kraus,et al.  Fluorescently labeled mesenchymal stem cells (MSCs) maintain multilineage potential and can be detected following implantation into articular cartilage defects. , 2002, Biomaterials.

[29]  D. Hungerford,et al.  Beta-1 integrin expression by human nasal chondrocytes in microcarrier spinner culture. , 2000, Journal of biomedical materials research.

[30]  M. Brittberg,et al.  Two- to 9-Year Outcome After Autologous Chondrocyte Transplantation of the Knee , 2000, Clinical orthopaedics and related research.

[31]  M. Brittberg,et al.  Autologous chondrocyte transplantation. , 1999, Clinical orthopaedics and related research.

[32]  A. Nixon,et al.  Enhanced repair of extensive articular defects by insulin‐like growth factor‐I‐laden fibrin composites , 1999, Journal of Orthopaedic Research.

[33]  W. B. van den Berg,et al.  Resurfacing potential of heterologous chondrocytes suspended in fibrin glue in large full-thickness defects of femoral articular cartilage: an experimental study in the goat. , 1999, Biomaterials.

[34]  M. Yaremchuk,et al.  Injectable tissue-engineered cartilage using a fibrin glue polymer. , 1999, Plastic and reconstructive surgery.

[35]  J. Buckwalter.  Articular cartilage: injuries and potential for healing. , 1998, The Journal of orthopaedic and sports physical therapy.

[36]  J Marler,et al.  Transplantation of cells in matrices for tissue regeneration. , 1998, Advanced drug delivery reviews.

[37]  Mark A. Randolph,et al.  Tissue Engineered Neocartilage Using Plasma Derived Polymer Substrates and Chondrocytes , 1998, Plastic and reconstructive surgery.

[38]  T. Ochi,et al.  Articular cartilage repair. Rabbit experiments with a collagen gel-biomatrix and chondrocytes cultured in it. , 1998, Acta orthopaedica Scandinavica.

[39]  J. Vacanti,et al.  De Novo Cartilage Generation Using Calcium Alginate‐Chondrocyte Constructs , 1996, Plastic and reconstructive surgery.

[40]  F. Silver,et al.  Preparation and use of fibrin glue in surgery. , 1995, Biomaterials.

[41]  C. Ohlsson,et al.  Treatment of deep cartilage defects in the knee with autologous chondrocyte transplantation. , 1994, The New England journal of medicine.

[42]  R J Todhunter,et al.  Chondrocyte‐fibrin matrix transplants for resurfacing extensive articular cartilage defects , 1994, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[43]  J. Bonaventure,et al.  Reexpression of cartilage-specific genes by dedifferentiated human articular chondrocytes cultured in alginate beads. , 1994, Experimental cell research.

[44]  W. B. van den Berg,et al.  Chondrocyte behavior in fibrin glue in vitro. , 1993, Acta orthopaedica Scandinavica.

[45]  M J Glimcher,et al.  Cell origin and differentiation in the repair of full-thickness defects of articular cartilage. , 1993, The Journal of bone and joint surgery. American volume.

[46]  R. Warren,et al.  The repair of osteochondral defects using an exogenous fibrin clot , 1992, The American journal of sports medicine.

[47]  T Ochi,et al.  Repair of rabbit articular surfaces with allograft chondrocytes embedded in collagen gel. , 1989, The Journal of bone and joint surgery. British volume.

[48]  H. Mankin,et al.  The response of articular cartilage to mechanical injury. , 1982, The Journal of bone and joint surgery. American volume.

[49]  M. Glimcher,et al.  Biochemical studies on repair cartilage resurfacing experimental defects in the rabbit knee. , 1980, The Journal of bone and joint surgery. American volume.