Microscale Atmospheric Pressure Plasma Jet as a Source for Plasma‐Driven Biocatalysis

The use of a microscale atmospheric pressure plasma jet (μAPPJ) was investigated for its potential to supply hydrogen peroxide in biocatalysis. Compared to a previously employed dielectric barrier discharge (DBD), the μAPPJ offered significantly higher H2O2 production rates and better handling of larger reaction volumes. The performance of the μAPPJ was evaluated with recombinant unspecific peroxygenase from Agrocybe aegerita (rAaeUPO). Using plasma‐treated buffer, no side reactions with other plasma‐generated species were detected. For long‐term treatment, rAaeUPO was immobilized, transferred to a rotating bed reactor, and reactions performed using the μAPPJ. The enzyme had a turnover of 36,415 mol mol−1 and retained almost full activity even after prolonged plasma treatment. Overall, the μAPPJ presents a promising plasma source for plasma‐driven biocatalysis.

[1]  N. Bibinov,et al.  Characterisation of volume and surface dielectric barrier discharges in N 2 –O 2 mixtures using optical emission spectroscopy , 2020, Plasma Processes and Polymers.

[2]  J. Bandow,et al.  Plasma‐Driven in Situ Production of Hydrogen Peroxide for Biocatalysis , 2020, ChemSusChem.

[3]  P. Awakowicz,et al.  Study on Chemical Modifications of Glutathione by Cold Atmospheric Pressure Plasma (Cap) Operated in Air in the Presence of Fe(II) and Fe(III) Complexes , 2019, Scientific Reports.

[4]  Mafalda Dias Gomes,et al.  Considerations when Measuring Biocatalyst Performance , 2019, Molecules.

[5]  A. Ngamjarurojana,et al.  A compact pulse-modulation cold air plasma jet for the inactivation of chronic wound bacteria: development and characterization , 2019, Heliyon.

[6]  A. Bogaerts,et al.  Applications of the COST Plasma Jet: More than a Reference Standard , 2019, Plasma.

[7]  I. Arends,et al.  Expanding the Spectrum of Light-Driven Peroxygenase Reactions , 2018, ACS catalysis.

[8]  A. Bogaerts,et al.  Combining experimental and modelling approaches to study the sources of reactive species induced in water by the COST RF plasma jet. , 2018, Physical chemistry chemical physics : PCCP.

[9]  Wuyuan Zhang,et al.  Selective Activation of C−H Bonds in a Cascade Process Combining Photochemistry and Biocatalysis , 2017, Angewandte Chemie.

[10]  Ronny Brandenburg,et al.  Dielectric barrier discharges: progress on plasma sources and on the understanding of regimes and single filaments , 2017 .

[11]  Frank Hollmann,et al.  Peroxygenases en route to becoming dream catalysts. What are the opportunities and challenges? , 2017, Current opinion in chemical biology.

[12]  J. Foster,et al.  Plasma–liquid interactions: a review and roadmap , 2016 .

[13]  Miles M. Turner,et al.  Concepts and characteristics of the ‘COST Reference Microplasma Jet’ , 2016 .

[14]  Frank Hollmann,et al.  Peroxygenase-Catalyzed Oxyfunctionalization Reactions Promoted by the Complete Oxidation of Methanol. , 2016, Angewandte Chemie.

[15]  R. Ludwig,et al.  Tandem-yeast expression system for engineering and producing unspecific peroxygenase. , 2015, Enzyme and microbial technology.

[16]  Frank Hollmann,et al.  Specific oxyfunctionalisations catalysed by peroxygenases: opportunities, challenges and solutions , 2015 .

[17]  K. Weltmann,et al.  Tracking plasma generated H2O2 from gas into liquid phase and revealing its dominant impact on human skin cells , 2014 .

[18]  N. Bibinov,et al.  Characterization of DBD plasma source for biomedical applications , 2009 .

[19]  Juergen F. Kolb,et al.  Cold atmospheric pressure air plasma jet for medical applications , 2008 .

[20]  J. Benedikt,et al.  Characterization of the effluent of a He/O2 microscale atmospheric pressure plasma jet by quantitative molecular beam mass spectrometry , 2010 .