Improved microcirculation imaging of human skin in vivo using optical microangiography with a correlation mapping mask

Abstract. Optical microangiography based on optical coherence tomography (OCT) is prone to noise that arises from a static tissue region. Here, we propose a method that can significantly reduce this noise. The method is developed based on an approach that uses the magnitude information of OCT signals to produce tissue microangiograms, especially suitable for the case where a swept-source OCT system is deployed. By combined use of two existing OCT microangiography methods—ultrahigh-sensitive optical microangiography (UHS-OMAG) and correlation mapping OCT (cmOCT)—the final tissue microangiogram is generated by masking UHS-OMAG image using the binary representation of cmOCT image. We find that this process masks the residual static artifacts while preserving the vessel structures. The noise rejection capability of the masked approach (termed as mOMAG) is tested on a tissue-like flow phantom as well as an in vivo human skin tissue. Compared to UHS-OMAG and cmOCT, we demonstrate that the proposed method is capable of achieving improved signal-to-noise ratio in providing microcirculation images. Finally, we show its clinical potential by quantitatively assessing the vascular difference between a burn scar and a normal skin of human subject in vivo.

[1]  Ruikang K Wang,et al.  Three-dimensional optical imaging of microvascular networks within intact lymph node in vivo. , 2010, Journal of biomedical optics.

[2]  Yih Miin Liew,et al.  In vivo assessment of human burn scars through automated quantification of vascularity using optical coherence tomography , 2012, Journal of biomedical optics.

[3]  Bernard Choi,et al.  High-resolution imaging of microvasculature in human skin in-vivo with optical coherence tomography , 2012, Optics express.

[4]  M. Simoons,et al.  The microcirculation in health and critical disease. , 2008, Progress in cardiovascular diseases.

[5]  Martin F. Kraus,et al.  Split-spectrum amplitude-decorrelation angiography with optical coherence tomography , 2012, Optics express.

[6]  J. Barton,et al.  Flow measurement without phase information in optical coherence tomography images. , 2005, Optics express.

[7]  T. Gambichler,et al.  Evaluation of the epidermal refractive index measured by optical coherence tomography , 2006, Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging.

[8]  Martin Leahy,et al.  In vivo imaging of the microcirculation of the volar forearm using correlation mapping optical coherence tomography (cmOCT) , 2011, Biomedical optics express.

[9]  Freddy T. Nguyen,et al.  Intraoperative evaluation of breast tumor margins with optical coherence tomography. , 2009, Cancer research.

[10]  Adrian Mariampillai,et al.  Speckle variance detection of microvasculature using swept-source optical coherence tomography. , 2008, Optics letters.

[11]  Paul P M van Zuijlen,et al.  Perspectives on burn scar evaluation and artificial skin , 2002 .

[12]  Robert J Zawadzki,et al.  Noninvasive imaging of the foveal avascular zone with high-speed, phase-variance optical coherence tomography. , 2012, Investigative ophthalmology & visual science.

[13]  Ruikang K. Wang,et al.  Depth-resolved imaging of capillary networks in retina and choroid using ultrahigh sensitive optical microangiography. , 2010, Optics letters.

[14]  Ruikang K. Wang,et al.  Three dimensional optical angiography. , 2007, Optics express.

[15]  Ruikang K. Wang,et al.  Volumetric In Vivo Imaging of Microvascular Perfusion Within the Intact Cochlea in Mice Using Ultra-High Sensitive Optical Microangiography , 2011, IEEE Transactions on Medical Imaging.

[16]  Bruce J. Tromberg,et al.  A comparison of Doppler optical coherence tomography methods , 2012, Biomedical optics express.

[17]  S. M. R. Motaghiannezam,et al.  Differential phase-contrast, swept-source optical coherence tomography at 1060 nm for in vivo human retinal and choroidal vasculature visualization. , 2012, Journal of biomedical optics.

[18]  Martin J Leahy,et al.  Microcirculation imaging based on full-range high-speed spectral domain correlation mapping optical coherence tomography , 2013, Journal of biomedical optics.

[19]  Ruikang K. Wang,et al.  Supercontinuum light source enables in vivo optical microangiography of capillary vessels within tissue beds. , 2011, Optics letters.

[20]  Benjamin J Vakoc,et al.  Fourier-domain optical coherence tomography: recent advances toward clinical utility. , 2009, Current opinion in biotechnology.

[21]  Benjamin J Vakoc,et al.  Three-dimensional microscopy of the tumor microenvironment in vivo using optical frequency domain imaging , 2009, Nature Medicine.

[22]  Boris Povazay,et al.  Multispectral in vivo three-dimensional optical coherence tomography of human skin. , 2010, Journal of biomedical optics.

[23]  T Ohtsuka,et al.  Quantitative analysis of nailfold capillary abnormalities in patients with connective tissue diseases , 1999, International journal of dermatology.

[24]  Clare Y L Chao,et al.  Microvascular dysfunction in diabetic foot disease and ulceration , 2009, Diabetes/metabolism research and reviews.

[25]  Wolfgang Drexler,et al.  In situ structural and microangiographic assessment of human skin lesions with high-speed OCT , 2012, Biomedical optics express.

[26]  Mohammad Sultan Mahmud,et al.  Review of speckle and phase variance optical coherence tomography to visualize microvascular networks , 2013, Journal of biomedical optics.

[27]  Freddy T. Nguyen,et al.  Optical coherence tomography: a review of clinical development from bench to bedside. , 2007, Journal of biomedical optics.

[28]  Ruikang K. Wang,et al.  Ultrahigh sensitive optical microangiography for in vivo imaging of microcirculations within human skin tissue beds. , 2010, Optics express.

[29]  Ruikang K. Wang,et al.  In vivo volumetric imaging of microcirculation within human skin under psoriatic conditions using optical microangiography , 2011, Lasers in surgery and medicine.

[30]  Ruikang K. Wang,et al.  Optical microangiography provides depth-resolved images of directional ocular blood perfusion in posterior eye segment. , 2010, Journal of biomedical optics.

[31]  Erik L Ritman,et al.  The human cutaneous circulation as a model of generalized microvascular function. , 2008, Journal of applied physiology.

[32]  Adrian Mariampillai,et al.  Real-time speckle variance swept-source optical coherence tomography using a graphics processing unit , 2012, Biomedical optics express.

[33]  Adrian Mariampillai,et al.  Optimized speckle variance OCT imaging of microvasculature. , 2010, Optics letters.

[34]  Hrebesh M. Subhash,et al.  High-speed high-sensitivity spectral-domain correlation mapping optical coherence tomography based modified scanning protocol , 2013, Photonics West - Biomedical Optics.

[35]  Ruikang K. Wang,et al.  Quantifying Optical Microangiography Images Obtained from a Spectral Domain Optical Coherence Tomography System , 2012, Int. J. Biomed. Imaging.

[36]  R. Leitgeb,et al.  Ultrahigh-speed non-invasive widefield angiography. , 2012, Journal of biomedical optics.

[37]  Robert J Zawadzki,et al.  Volumetric microvascular imaging of human retina using optical coherence tomography with a novel motion contrast technique. , 2009, Optics express.

[38]  Ruikang K. Wang,et al.  High-resolution wide-field imaging of retinal and choroidal blood perfusion with optical microangiography. , 2010, Journal of biomedical optics.

[39]  K. Ueda,et al.  Study of microvascular structure in keloid and hypertrophic scars: Density of microvessels and the efficacy of three-dimensional vascular imaging , 2010, Journal of plastic surgery and hand surgery.

[40]  Yuhei Takahashi,et al.  Graphics processing unit accelerated intensity-based optical coherence tomography angiography using differential frames with real-time motion correction , 2013, Journal of biomedical optics.

[41]  Ruikang K. Wang,et al.  A practical approach to eliminate autocorrelation artefacts for volume-rate spectral domain optical coherence tomography , 2006, Physics in medicine and biology.