THREE‐DIMENSIONAL RECONSTRUCTION OF BENIGN LYMPHOID AGGREGATES IN BONE MARROW TREPHINES

Distinguishing between deposits of low‐grade non‐Hodgkin's lymphoma and benign lymphoid aggregates in bone marrow trephine sections is a recognized problem in haematopathology. To test the hypothesis that benign lymphoid aggregates do not make contact with a trabecular surface, three‐dimensional models were constructed of five serially sectioned bone marrow trephines, containing a total of 19 lymphoid aggregates known to be benign. The computer‐generated images showed that benign lymphoid aggregates were located in the central marrow space and did not become paratrabecular. This suggests that a paratrabecular location may help in some cases to distinguish deposits of low‐grade non‐Hodgkin's lymphoma from benign lymphoid aggregates.

[1]  M. Higuchi,et al.  Lymph Nodules in Human Bone Marrow , 1957 .

[2]  A. Rywlin,et al.  Lymphoid nodules of bone marrow: normal and abnormal. , 1974, Blood.

[3]  R. Greil,et al.  IMMUNOHISTOLOGY FOR QUANTIFICATION OF NORMAL BONE MARROW LYMPHOCYTE SUBSETS , 1989, British journal of haematology.

[4]  G. Hoffmann-Fezer,et al.  Lymphoproliferations in the bone marrow: identification and evolution, classification and staging. , 1984, Journal of clinical pathology.

[5]  P. Isaacson,et al.  Bone marrow trephine biopsy in lymphoproliferative disease. , 1992, Journal of clinical pathology.

[6]  M H Deverell,et al.  Three‐dimensional reconstruction of human embryonic notochords: Clue to the pathogenesis of chordoma , 1993, The Journal of pathology.

[7]  A. Pich,et al.  Lymphoid nodules and nodular lymphoid hyperplasia in bone marrow biopsies. , 1985, Acta haematologica.

[8]  G. Janossy,et al.  Immuno‐histological analysis of bone marrow involvement in lymphoproliferative disorders , 1982, British journal of haematology.

[9]  R. Warnke,et al.  The immunologic phenotyping of bone marrow biopsies and aspirates: frozen section techniques. , 1982, Blood.

[10]  B. Faulkner-Jones,et al.  Lymphoid aggregates in bone marrow: study of eventual outcome. , 1988, Journal of clinical pathology.

[11]  R. Bartl,et al.  Assessment of bone marrow histology in the malignant lymphomas (non‐Hodgkin's): correlation with clinical factors for diagnosis, prognosis, classification and staging , 1982, British journal of haematology.

[12]  P. Bishop,et al.  Bone marrow trephine biopsy in lymphoproliferative disease. , 1993, Journal of clinical pathology.

[13]  W F Whimster,et al.  Progress in computer‐generated three‐dimensional reconstruction , 1993, The Journal of pathology.

[14]  M. Leyland,et al.  Benign and malignant (B cell) focal lymphoid aggregates in bone marrow trephines shown by means of an immunogold-silver technique. , 1986, Journal of clinical pathology.

[15]  J G Holman,et al.  Applications of a microcomputer-based reconstruction system to produce realistic three-dimensional shaded images from serial sections. , 1989, Medical informatics = Medecine et informatique.

[16]  B. Falini,et al.  Immunohistological analysis of human bone marrow trephine biopsies using monoclonal antibodies , 1984, British journal of haematology.