Trapping Biases of Culex torrentium and Culex pipiens Revealed by Comparison of Captures in CDC Traps, Ovitraps, and Gravid Traps

ABSTRACT: We evaluate three trapping methods for their effectiveness at capturing Culex pipiens and Culex torrentium, both enzootic vectors of bird-associated viruses in Europe. The comparisons, performed in two regions in Sweden, were among CDC traps baited with carbon dioxide, gravid traps, and ovitraps baited with hay infusion. The proportions of the two Culex species in a catch differed between trap types, with CDC traps catching a lower proportion of Cx. torrentium than both gravid traps and ovitraps. Between gravid traps and ovitraps, there was no difference in the proportions of the two species. The results indicate that Cx. torrentium may go undetected or underestimated compared to Cx. pipiens when using carbon dioxide baited CDC traps. The new insight of trap bias presented here adds an important dimension to consider when investigating these vectors of bird-associated viruses in the field.

[1]  A. Larsson,et al.  Exceptional Sindbis virus infection rate in Swedish Culex torrentium defines its role as a major enzootic vector , 2015 .

[2]  N. Becker,et al.  Field evaluation of four widely used mosquito traps in Central Europe , 2014, Parasites & Vectors.

[3]  E. Scholte,et al.  The arbovirus vector Culex torrentium is more prevalent than Culex pipiens in northern and central Europe , 2014, Medical and veterinary entomology.

[4]  N. Becker,et al.  First Nationwide Surveillance of Culex pipiens Complex and Culex torrentium Mosquitoes Demonstrated the Presence of Culex pipiens Biotype pipiens/molestus Hybrids in Germany , 2013, PloS one.

[5]  A. Halling,et al.  The geographic distribution of mosquito species in Sweden , 2013 .

[6]  D. Fontenille,et al.  Comparison of Different Trapping Methods for Surveillance of Mosquito Vectors of West Nile Virus in Rhône Delta, France , 2012, Journal of vector ecology : journal of the Society for Vector Ecology.

[7]  B. Caputo,et al.  Looking for the Gold Standard: Assessment of the Effectiveness of four Traps for Monitoring Mosquitoes in Italy , 2012, Journal of vector ecology : journal of the Society for Vector Ecology.

[8]  J. Minár,et al.  Mosquitoes (Diptera: Culicidae) of the Lower Dyje River Basin (Podyjí) at the Czech-Austrian border , 2012, Central European Journal of Biology.

[9]  O. Ostman,et al.  Geographic distribution and relative abundance of the sibling vector species Culex torrentium and Culex pipiens in Sweden. , 2011, Vector borne and zoonotic diseases.

[10]  B. Kesavaraju,et al.  Efficacy of Gravid Traps in Trapping Culex pipiens , 2011, Journal of the American Mosquito Control Association.

[11]  Ho‐Sheng Wu,et al.  Comparison of the Efficacy of CO2-Baited and Unbaited Light Traps, Gravid Traps, Backpack Aspirators, and Sweep Net Collections for Sampling Mosquitoes Infected with Japanese Encephalitis Virus , 2011, Journal of vector ecology : journal of the Society for Vector Ecology.

[12]  N. Becker,et al.  Distribution and frequency of Culex pipiens and Culex torrentium (Culicidae) in Europe and diagnostic allozyme markers , 2011 .

[13]  R. Bellini,et al.  Evidence of Simultaneous Circulation of West Nile and Usutu Viruses in Mosquitoes Sampled in Emilia-Romagna Region (Italy) in 2009 , 2010, PloS one.

[14]  P. Erixon,et al.  A sensitive and reliable restriction enzyme assay to distinguish between the mosquitoes Culex torrentium and Culex pipiens , 2010, Medical and veterinary entomology.

[15]  M. Bogojević,et al.  Seasonal dynamics of mosquitoes (Diptera: Culicidae) in Osijek (Croatia) for the period 1995–2004 , 2009, Biologia.

[16]  S. Ritchie,et al.  Efficacy of bird-baited traps placed at different heights for collecting ornithophilic mosquitoes in eastern Queensland, Australia , 2009 .

[17]  John B. Silver,et al.  Mosquito Ecology: Field Sampling Methods , 2008 .

[18]  H. Zeller,et al.  Vector competence of some French Culex and Aedes mosquitoes for West Nile virus. , 2008, Vector borne and zoonotic diseases.

[19]  N. Burkett-Cadena,et al.  Comparison of Infusions of Commercially Available Garden Products for Collection of Container-Breeding Mosquitoes , 2008, Journal of the American Mosquito Control Association.

[20]  A. Allepuz,et al.  Usutu Virus Sequences in Culex pipiens (Diptera: Culicidae), Spain , 2008, Emerging infectious diseases.

[21]  Gregory M. Williams,et al.  Comparison of light traps, gravid traps, and resting boxes for West Nile virus surveillance , 2007, Journal of vector ecology : journal of the Society for Vector Ecology.

[22]  R. Novak,et al.  Evaluation of Four Sampling Techniques for Surveillance of Culex quinquefasciatus (Diptera: Culicidae) and Other Mosquitoes in African Rice Agroecosystems , 2007, Journal of medical entomology.

[23]  D. Giberson,et al.  Mosquito species composition, phenology and distribution (Diptera: Culicidae) on Prince Edward Island , 2007 .

[24]  D. Barnard,et al.  Efficacy of Four Trap Types for Detecting and Monitoring Culex spp. in North Central Florida , 2006, Journal of medical entomology.

[25]  G. Glass,et al.  COMPARISON OF MOSQUITO TRAPPING METHOD EFFICACY FOR WEST NILE VIRUS SURVEILLANCE IN NEW MEXICO , 2006, Journal of the American Mosquito Control Association.

[26]  G. Glass,et al.  Targeted Trapping of Mosquito Vectors in the Chesapeake Bay Area of Maryland , 2006, Journal of medical entomology.

[27]  U. Bernier,et al.  Laboratory Evaluation of Avian Odors for Mosquito (Diptera: Culicidae) Attraction , 2006, Journal of medical entomology.

[28]  B. Jackson,et al.  OVIPOSITION PREFERENCES OF CULEX RESTUANS AND CULEX PIPIENS (DIPTERA: CULICIDAE) FOR SELECTED INFUSIONS IN OVIPOSITION TRAPS AND GRAVID TRAPS , 2005, Journal of the American Mosquito Control Association.

[29]  P. Weinstein,et al.  Evaluation of two dipping methods for sampling immature Culex and Ochlerotatus mosquitoes (Diptera: Culicidae) from artificial containers , 2005 .

[30]  U. Bernier,et al.  EVALUATION OF OVIPOSITION SUBSTRATES AND ORGANIC INFUSIONS ON COLLECTION OF CULEX IN FLORIDA , 2005, Journal of the American Mosquito Control Association.

[31]  A. Vaheri,et al.  Causative Agent of Pogosta Disease Isolated from Blood and Skin Lesions , 2004, Emerging infectious diseases.

[32]  Marjorie J. Wonham,et al.  An epidemiological model for West Nile virus: invasion analysis and control applications , 2004, Proceedings of the Royal Society of London. Series B: Biological Sciences.

[33]  W. Walton,et al.  Larval behavior of four Culex (Diptera: Culicidae) associated with treatment wetlands in the southwestern United States. , 2003, Journal of vector ecology : journal of the Society for Vector Ecology.

[34]  N. Becker,et al.  Mosquitoes and Their Control , 2003, Springer US.

[35]  N. Becker,et al.  Preliminary studies on the mosquito fauna of Luxembourg , 2003 .

[36]  W. Takken,et al.  Comparison of carbon dioxide‐baited trapping systems for sampling outdoor mosquito populations in Tanzania , 2000, Medical and veterinary entomology.

[37]  W. Takken,et al.  Sampling Gravid Culex quinquefasciatus (Diptera: Culicidae) in Tanzania with Traps Baited with Synthetic Oviposition Pheromone and Grass Infusions , 2000, Journal of medical entomology.

[38]  R. Lanciotti,et al.  Entomologic and avian investigations of an epidemic of West Nile fever in Romania in 1996, with serologic and molecular characterization of a virus isolate from mosquitoes. , 1999, The American journal of tropical medicine and hygiene.

[39]  J. Lundström.  Mosquito-borne viruses in western Europe: a review. , 1999, Journal of vector ecology : journal of the Society for Vector Ecology.

[40]  J. O. Lunstrom.  Vector competence of western european mosquitoes for arboviruses: a review of field and experimental studies , 1994 .

[41]  M. Turell,et al.  Effect of environmental temperature on the vector competence of Culex pipiens and Cx. torrentium for Ockelbo virus. , 1990, The American journal of tropical medicine and hygiene.

[42]  B. Niklasson,et al.  Swedish Culex torrentium and Cx. pipiens (Diptera: Culicidae) as experimental vectors of Ockelbo virus. , 1990, Journal of medical entomology.

[43]  T. Jaenson.  Vector roles of Fennoscandian mosquitoes attracted to mammals, birds and frogs , 1990, Medical and veterinary entomology.

[44]  T. Struppe.  Biologie und Ökologie von Culex torrentium Martini unter besonderer Berücksichtigung seiner Beziehungen im menschlichen Siedlungsbereich , 1989 .

[45]  W. Reisen,et al.  Effectiveness of five methods for sampling adult Culex mosquitoes in rural and urban habitats in San Bernardino County, California. , 1987, Journal of the American Mosquito Control Association.

[46]  T. Jaenson,et al.  Feeding patterns of mosquitoes (Diptera: Culicidae) in relation to the transmission of Ockelbo disease in sweden , 1986 .

[47]  P. Reiter.  A portable battery-powered trap for collecting gravid Culex mosquitoes. , 1983 .

[48]  M. Skogh,et al.  OCKELBO DISEASE: EPIDEMIC ARTHRITIS-EXANTHEMA SYNDROME IN SWEDEN CAUSED BY SINDBIS-VIRUS LIKE AGENT , 1982, The Lancet.