Heterogeneity of human colony-forming cells (CFU-C): differences in size, rate of colony formation, and responsiveness to colony-stimulating factor.

Human bone marrow cells have been fractionated by velocity sedimentation at unit gravity. Fractions were analyzed for cell morphology, number of nucleated cells and myeloid colony-forming cells (CFU-C's). Colony formation was assayed with the following CSF preparations: serum-free HLCM and two electrophoretically distinct CSF fractions (CSF-A and CSF-B) purified from the same source. Two distinct CFU-C populations were found. One, a rapidly sedimenting (7.2 to 8.0 mm/hr), population exhibited colonies after 7 days of culture in response to HLCM and CSF-A only. The second, a more slowly sedimenting (6.5 mm/hr) CFU-C peak, did not exhibit colonies until 11 days of culture and did so in response to all three CSF's tested. The results indicate that human bone marrow CFU-C's are heterogeneous and that the two purified CSF fractions from human lung have different CFU-C specificity.

[1]  S. Inoue,et al.  Heterogeneity of human colony-forming cells. , 1978, Blood.

[2]  G. Johnson,et al.  Heterogeneity in human neutrophil, macrophage and eosinophil progenitor cells demonstrated by velocity sedimentation separation. , 1977, Blood.

[3]  A. Yunis,et al.  The isolation and characterization of a colony stimulating factor from human lung. , 1977, Biochimica et biophysica acta.

[4]  B. Kubanek,et al.  THE IN VITRO DIFFERENTIATION OF DENSITY SUB‐POPULATIONS OF COLONY‐FORMING CELLS UNDER THE INFLUENCE OF DIFFERENT TYPES OF COLONY‐STIMULATING FACTOR , 1977, Cell and tissue kinetics.

[5]  G. Johnson,et al.  Eosinophil colony formation in semisolid cultures of human bone marrow cells. , 1977, Blood.

[6]  H. Macdonald,et al.  Heterogeneity of in vitro colony‐ and cluster‐forming cells in the mouse marrow: Segregation by velocity sedimentation , 1975, Journal of cellular physiology.

[7]  J. Till,et al.  The isolation and properties of granulocytic colony-stimulating activities from medium conditioned by human peripheral leucocytes. , 1975, The Biochemical journal.

[8]  M. Moore,et al.  Effect of chloramphenicol and thiamphenicol on the in vitro colony-forming cell. , 1974, Blood.

[9]  D. Metcalf,et al.  A low molecular weight factor in lung‐conditioned medium stimulating granulocyte and monocyte colony formation in vitro , 1973, Journal of cellular physiology.

[10]  J. Till,et al.  Physical separation of hemopoietic stem cells from cells forming colonies in culture , 1969, Journal of cellular physiology.

[11]  R. A. Phillips,et al.  Separation of cells by velocity sedimentation , 1969, Journal of cellular physiology.

[12]  D. Metcalf,et al.  The growth of mouse bone marrow cells in vitro. , 1966, The Australian journal of experimental biology and medical science.