Seasonal variations of ectomycorrhizal communities in declining Quercus ilex forests: interactions with topography, tree health status and Phytophthora cinnamomi infections

SeasonalvariationsinectomycorrhizalsymbiosisofQuercusilextreeswerestudied,withspecialemphasisontree health status, Phytophthora cinnamomi root infections and topography. Five Q. ilex forests in western Spain were selected,andca.40 000roottipsfrom3decliningand3non-decliningtreesperforestwereexamined.Ectomycorrhizal fungal abundance and P. cinnamomi root infections were quantified seasonally for 2 years, and an assessment was made of the water soil content and physiological status of trees during summer, the most stressful season. Seasonal changes in ectomycorrhizal fungal abundance were higher for Tomentella spp. than those for Russula spp. and Cenococcum geophilum, but these changes were not influenced by topography, tree decline status and P. cinnamomi root infections. Seasonal variations of non-vital tips were conditioned significantly by the decline status of trees. A higher proportion of non-vital tips and a lower proportion of vital non-mycorrhizal tips were observed in declining than in non-declining trees. Abundance of C. geophilum and Tomentella spp. was not influenced byP. cinnamomi root infections but by topography. Abundance of Russula spp. was not influenced byP.cinnamomirootinfectionsbutbythedeclinestatusoftrees.Therelationshipsfoundbetweentreephysiology and ectomycorrhizalabundance changed significantly depending on the decline status of trees. For thefirsttime, seasonal variations in ectomycorrhizae inP. cinnamomi-infectedQ. ilexforests are reported. The lowdiversityand abundance of ectomycorrhizal fungi associated with declining trees should be further studied in order to uncover causes or consequences of Q. ilex crown transparency.

[1]  F. Bussotti,et al.  Part IV Visual assessment of crown condition and damaging agents , 2016 .

[2]  S. Woodward,et al.  Influence of temperature on germination of Quercus ilex in Phytophthora cinnamomi, P. gonapodyides, P. quercina and P. psychrophila infested soils , 2015 .

[3]  G. Moreno,et al.  Drought events determine performance of Quercus ilex seedlings and increase their susceptibility to Phytophthora cinnamomi , 2014 .

[4]  G. Moreno,et al.  Ectomycorrhizal symbiosis in declining and non-declining Quercus ilex trees infected with or free of Phytophthora cinnamomi , 2014 .

[5]  A. Chambery,et al.  Strategies of attack and defence in woody plant-Phytophthora interactions , 2014 .

[6]  A. Marisa,et al.  Valuing native ectomycorrhizal fungi as a Mediterranean forestry component for sustainable and innovative solutions1 , 2014 .

[7]  L. Montecchio,et al.  The ectomycorrhizal community in Mediterranean old-growth Quercus ilex forests along an altitudinal gradient , 2014 .

[8]  R. Koide,et al.  Determining place and process: functional traits of ectomycorrhizal fungi that affect both community structure and ecosystem function. , 2014, The New phytologist.

[9]  D. Ratkowsky,et al.  Temperate eucalypt forest decline is linked to altered ectomycorrhizal communities mediated by soil chemistry , 2013 .

[10]  G. Moreno,et al.  Combined effects of soil properties and Phytophthora cinnamomi infections on Quercus ilex decline , 2013, Plant and Soil.

[11]  A. Franceschini,et al.  Studies on the ectomycorrhizal community in a declining Quercus suber L. stand , 2013, Mycorrhiza.

[12]  M. Clara,et al.  Decline of Mediterranean oak trees and its association with Phytophthora cinnamomi: a review , 2013, European Journal of Forest Research.

[13]  G. Moreno,et al.  Quercus ilex forests are influenced by annual variations in water table, soil water deficit and fine root loss caused by Phytophthora cinnamomi , 2013 .

[14]  P. Scott,et al.  Relationships between the crown health, fine root and ectomycorrhizae density of declining Eucalyptus gomphocephala , 2013, Australasian Plant Pathology.

[15]  B. Dell,et al.  Seedling mycorrhizal type and soil chemistry are related to canopy condition of Eucalyptus gomphocephala , 2013, Mycorrhiza.

[16]  Jian Zhang,et al.  Ectomycorrhizal fungal communities of Quercus liaotungensis along local slopes in the temperate oak forests on the Loess Plateau, China , 2013, Ecological Research.

[17]  E. D. Maso,et al.  Detecting asymptomatic ink‐diseased chestnut trees by the composition of the ectomycorrhizal community , 2012 .

[18]  L. Coll,et al.  Fine Root Seasonal Dynamics, Plasticity, and Mycorrhization in 2 Coexisting Mediterranean Oaks with Contrasting Aboveground Phenology , 2012 .

[19]  J. Kinal,et al.  Temporal and spatial soil inoculum dynamics following Phytophthora cinnamomi invasion of Banksia woodland and Eucalyptus marginata forest biomes of south-western Australia , 2010, Australasian Plant Pathology.

[20]  G. Hardy,et al.  False-negative isolations or absence of lesions may cause mis-diagnosis of diseased plants infected with Phytophthora cinnamomi , 2000, Australasian Plant Pathology.

[21]  J. P. Sousa,et al.  Fungal fruitbodies and soil macrofauna as indicators of land use practices on soil biodiversity in Montado , 2011, Agroforestry Systems.

[22]  R. Joffre,et al.  Ectomycorrhizal communities in a Mediterranean forest ecosystem dominated by Quercus ilex: seasonal dynamics and response to drought in the surface organic horizon , 2011, Annals of Forest Science.

[23]  A. Pérez-Sierra,et al.  First report of Phytophthora gonapodyides involved in the decline of Quercus ilex in xeric conditions in Spain , 2010 .

[24]  G. Moreno,et al.  Evaluating potassium phosphonate injections for the control of Quercus ilex decline in SW Spain: implications of low soil contamination by Phytophthora cinnamomi and low soil water content on the effectiveness of treatments , 2009, Phytoparasitica.

[25]  D. Godbold,et al.  Ectomycorrhizal community structure in a healthy and a Phytophthora-infected chestnut (Castanea sativa Mill.) stand in central Italy , 2009, Mycorrhiza.

[26]  G. Moreno,et al.  Quercus ilex root growth in response to heterogeneous conditions of soil bulk density and soil NH4-N content. , 2009 .

[27]  G. Moreno,et al.  The Functioning, Management and Persistence of Dehesas , 2009 .

[28]  J. Pierrat,et al.  Temporal Changes in the Ectomycorrhizal Community in Two Soil Horizons of a Temperate Oak Forest , 2008, Applied and Environmental Microbiology.

[29]  L. Montecchio,et al.  Short-term effect of removing tree competition on the ectomycorrhizal status of a declining pedunculate oak forest (Quercus robur L.) , 2007 .

[30]  R. Koide,et al.  On temporal partitioning of a community of ectomycorrhizal fungi. , 2007, The New phytologist.

[31]  J. Churin,et al.  Differential ability of ectomycorrhizas to survive drying , 2007, Mycorrhiza.

[32]  N. Breda,et al.  Temperate forest trees and stands under severe drought: a review of ecophysiological responses, adaptation processes and long-term consequences , 2006 .

[33]  F. Richard,et al.  Diversity and specificity of ectomycorrhizal fungi retrieved from an old-growth Mediterranean forest dominated by Quercus ilex. , 2005, The New phytologist.

[34]  M. Román,et al.  Post-fire, seasonal and annual dynamics of the ectomycorrhizal community in a Quercus ilex L. forest over a 3-year period , 2005, Mycorrhiza.

[35]  R. Koide,et al.  Evidence of species interactions within an ectomycorrhizal fungal community. , 2004, The New phytologist.

[36]  V. Claveria,et al.  Análisis de la comunidad ectomicorrícica de un carrascal de Navarra (España) , 2005 .

[37]  S. Rossi,et al.  Changes in Ectomycorrhizal Diversity in a Declining "Quercus ilex" Coastal Forest , 2004 .

[38]  A. Nardini,et al.  Different responses to drought and freeze stress of Quercus ilex L. growing along a latitudinal gradient , 2000, Plant Ecology.

[39]  K. Saikkonen,et al.  SEVERE DEFOLIATION OF SCOTS PINE REDUCES REPRODUCTIVE INVESTMENT BY ECTOMYCORRHIZAL SYMBIONTS , 2003 .

[40]  J. R. Harris,et al.  The Efficacy of Ectomycorrhizal Colonization of Pin and Scarlet Oak in Nursery Production , 2003 .

[41]  M. Desprez-Loustau,et al.  Root infection by Phytophthora cinnamomi in seedlings of three oak species , 2001 .

[42]  A. Morte,et al.  Effect of drought stress on growth and water relations of the mycorrhizal association Helianthemum almeriense-Terfezia claveryi , 2000, Mycorrhiza.

[43]  A. Pisi,et al.  Effect of ectomycorrhizal fungi on chestnut ink disease , 1999, Mycorrhiza.

[44]  M. Desprez-Loustau,et al.  First record of Phytophthora cinnamomi on cork and holm oaks in France and evidence of pathogenicity , 1998 .

[45]  T. Jung,et al.  Isolation, identification and pathogenicity of Phytophthora species from declining oak stands , 1996 .

[46]  M. Ashmore,et al.  Nutrient relations and root mycorrhizal status of healthy and declining beech (Fagus sylvatica L.) in Southern Britain , 1996 .

[47]  H. Blaschke Decline symptoms on roots of Quercus robur , 1994 .

[48]  J. Colpaert,et al.  The growth of the extramatrical mycelium of ectomycorrhizal fungi and the growth response of Pinus sylvestris L. , 1992 .

[49]  N. Malajczuk Interaction between Phytophthora cinnamomi zoospores and micro-organisms on non-mycorrhizal and ectomycorrhizal roots of Eucalyptus marginata , 1988 .

[50]  M. Larsen,et al.  Distribution of Ectomycorrhizae in a Mature Douglas-fir/Larch Forest Soil in Western Montana , 1976 .

[51]  D. Marx Ectomycorrhizae as biological deterrents to pathogenic root infections. , 1972, Annual review of phytopathology.

[52]  L. Grand,et al.  THE NATURAL OCCURRENCE OF AN ANTIBIOTIC IN THE MYCORRHIZAL FUNGUS GENOCOCCUM GRANIFORME. , 1964, Canadian journal of microbiology.