Distribution of ice nucleation-active bacteria on plants in nature

A replica plating method for rapid quantitation of ice nucleation-active (INA) bacteria was developed. Leaf washings of plant samples from California, Colorado, Florida, Louisiana, and Wisconsin were tested for the presence of INA bacteria. Of the 95 plant species sampled, 74 were found to harbor INA bacteria. Only the conifers were, as a group, unlikely to harbor INA bacteria. All of the INA bacteria isolated resembled either Pseudomonas syringae or Erwinia herbicola. Sufficient numbers of INA bacteria were present on the samples to account for the ice nuclei associated with leaves that are necessary for freezing injury to occur. Numbers of INA bacteria were large enough to suggest that plant surfaces may constitute a significant source of atmospheric ice nuclei.

[1]  S. Lindow,et al.  THE ROLE OF BACTERIAL ICE NUCLEI IN FROST INJURY TO SENSITIVE PLANTS , 1978 .

[2]  G. Vali,et al.  Biogenic Ice Nuclei: Part I. Terrestrial and Marine Sources , 1976 .

[3]  Gabor Vali,et al.  Biogenic Ice Nuclei. Part II: Bacterial Sources , 1976 .

[4]  S. Lindow,et al.  Frost sensitivity of Zea mays increased by application of Pseudomonas syringae , 1976, Nature.

[5]  J. Crosse,et al.  Host relations and distribution of a physiological and pathological variant of Pseudomonas morsprunorum , 1975 .

[6]  M. Goodfellow,et al.  Quantitative and Qualitative Studies of Phylloplane Bacteria from Lolium perenne , 1975 .

[7]  S. Kaku.  Analysis of freezing temperature distribution in plants. , 1975, Cryobiology.

[8]  W. Dowler.  Isolation and Characterization of Fluorescent Pseudomonads from Apparently Healthy Peach Trees , 1975 .

[9]  G. Ercolani.  Epiphytic Survival of Pseudomonas syringae on Hairy Vetch in Relation to Epidemiology of Bacterial Brown Spot of Bean in Wisconsin , 1974 .

[10]  S. Hsieh.  Suppressing Effects of Erwinia herbicola on Infection by Xanthomonas oryzae and on Symptom Development in Rice , 1974 .

[11]  B. Kennedy,et al.  Population changes of Pseudomonas glycinea on germinating soybean seeds. , 1974 .

[12]  C. Leben.  A Pathogenic Pseudomonad from Healthy Field-Grown Soybean Plants , 1973 .

[13]  E. Klos,et al.  Relationship of Erwinia herbicola to Erwinia amylovora , 1972 .

[14]  G. Vali.  Quantitative Evaluation of Experimental Results an the Heterogeneous Freezing Nucleation of Supercooled Liquids , 1971 .

[15]  M. Schroth,et al.  Colonization and movement of Pseudomonas syringae on healthy Bean seedlings. , 1970 .

[16]  M. Schroth,et al.  Erwinia-Induced Internal Necrosis of Immature Cotton Bolls , 1970 .

[17]  A. K. Chatterjee,et al.  Some observations on the physiology of Erwinia herbicola and its possible implication as a factor antagonistic to Erwinia amylovora in the "fire-blight" syndrome. , 1969, Canadian journal of microbiology.

[18]  J. Everton,et al.  Observations on the taxonomy of chromogenic bacteria isolated from cannery environments. , 1969, The Journal of applied bacteriology.

[19]  V. Rusch,et al.  The colonization of Soybean buds by Pseudomonas glycinea and other bacteria. , 1968 .

[20]  M. Matsuno.  Studies on Yellow Bacteria Inhabiting the Surface of Newly Croped Rice , 1967 .

[21]  J. Crosse.  Epidemiological Relations of the Pseudomonad Pathogens of Deciduous Fruit Trees , 1966 .

[22]  F. Deighton.  The non-parasitic microflora on the surfaces of living leaves , 1965 .

[23]  C. Duncan,et al.  COLIFORMS ASSOCIATED WITH SUGARCANE PLANTS AND JUICES. , 1964, Applied microbiology.

[24]  E. Geldreich,et al.  OCCURRENCE OF COLIFORMS, FECAL COLIFORMS, AND STREPTOCOCCI ON VEGETATION AND INSECTS. , 1964, Applied microbiology.

[25]  J. Crosse.  Bacterial canker of stone-fruits.: V. A comparison of leaf-surface populations of Pseudomonas mors-prunorum in autumn on two cherry varieties , 1963 .

[26]  J. Stout.  Bacteria of soil and pasture leaves at Claudelands Showgrounds , 1960 .

[27]  J. Crosse.  BACTERIAL CANKER OF STONE‐FRUITS: IV. INVESTIGATION OF A METHOD FOR MEASURING THE INOCULUM POTENTIAL OF CHERRY TREES , 1959 .

[28]  R. Rosenberger,et al.  Bacteriological changes in silage made at controlled temperatures. , 1958, Journal of general microbiology.

[29]  N. James.  Yellow chromogenic bacteria on wheat. II. Determinative studies. , 1955, Canadian journal of microbiology.

[30]  E. King,et al.  Two simple media for the demonstration of pyocyanin and fluorescin. , 1954, The Journal of laboratory and clinical medicine.

[31]  J. Lederberg,et al.  REPLICA PLATING AND INDIRECT SELECTION OF BACTERIAL MUTANTS , 1952, Journal of bacteriology.

[32]  A. Lochhead,et al.  BACTERIA ASSOCIATED WITH SEEDS OF VARIOUS CROP PLANTS , 1951 .

[33]  S. T. Cowan.  Bergey's Manual of Determinative Bacteriology , 1948, Nature.

[34]  J. Wilson,et al.  The microflora of stored wheat. , 1946, Canadian journal of research.