Imaging of melanin distribution using multiphoton autofluorescence decay curves

Background/purpose: Multiphoton fluorescence lifetime imaging (FLIM) is a technique that produces an image based on differences in the decay rate of fluorescence from a sample. Based on this method, the DermaInspect was developed to observe human skin components non‐invasively. In this study, we used the DermaInspect to study melanin in skin.