Techniques for the analysis of complex genomes

YAC Library Construction and Vectors. Mapping Clones and Localization of Probes. YACs as Probes for cDNA Identification (Cloning of Islands). Homologous Recombination (Integration of Vectors, Neo Targetting). Building YACs Host Stains and Stability. Cloning Telomers. Olgio and Genome Mapping. P1 Cloning. Microdisection. Telomer Structure and Overview. Genome PFGE. SDS Approach to Olgio and Genome Mapping.