Changes of skin collagen orientation associated with chronological aging as probed by polarized-FTIR micro-imaging.

During chronological skin aging, alterations in dermal structural proteins cause morphological modifications. Modifications are probably due to collagen fiber (type I collagen) rearrangement and reorientation with aging that have not been researched until now. FTIR microspectroscopy appears as an interesting method to study protein structure under normal and pathological conditions. Associated with a polarizer, this vibrational technique permits us to probe collagen orientation within skin tissue sections, by computing the ratio of integrated intensities of amide I and amide II bands. In this study, we used the polarized-FTIR imaging to evaluate molecular modifications of dermal collagen during chronological aging. The data processing of polarized infrared data revealed that type I collagen fibers become parallel to the skin surface in aged skin dermis. Our approach could find innovative applications in dermatology as well as in cosmetics.

[1]  F. Kremer,et al.  Structure-property relationships in major ampullate spider silk as deduced from polarized FTIR spectroscopy , 2007, The European physical journal. E, Soft matter.

[2]  Michel Manfait,et al.  Discriminating nevus and melanoma on paraffin-embedded skin biopsies using FTIR microspectroscopy. , 2005, Biochimica et biophysica acta.

[3]  Michel Manfait,et al.  Infrared imaging as a cancer diagnostic tool: Introducing a new concept of spectral barcodes for identifying molecular changes in colon tumors , 2013, Cytometry Part A.

[4]  H Zahouani,et al.  Non contact method for in vivo assessment of skin mechanical properties for assessing effect of ageing. , 2012, Medical engineering & physics.

[5]  H. Wulf,et al.  Water and Protein Structure in Photoaged and Chronically Aged Skin , 1998 .

[6]  R. Potts,et al.  A noninvasive, in vivo technique to quantitatively measure water concentration of the stratum corneum using attenuated total-reflectance infrared spectroscopy , 2004, Archives of Dermatological Research.

[7]  J. Ortonne,et al.  Histopathological characterization of primary cutaneous melanoma using infrared microimaging: a proof‐of‐concept study , 2010, The British journal of dermatology.

[8]  X. Bi,et al.  A novel method for determination of collagen orientation in cartilage by Fourier transform infrared imaging spectroscopy (FT-IRIS). , 2005, Osteoarthritis and cartilage.

[9]  H. Martens,et al.  Extended Multiplicative Signal Correction as a Tool for Separation and Characterization of Physical and Chemical Information in Fourier Transform Infrared Microscopy Images of Cryo-Sections of Beef Loin , 2005, Applied spectroscopy.

[10]  V. Duance,et al.  Type I and III collagen content and fibre distribution in normal human skin during ageing , 1987, The British journal of dermatology.

[11]  J Serup,et al.  Ultrasound structure and digital image analysis of the subepidermal low echogenic band in aged human skin: diurnal changes and interindividual variability. , 1994, The Journal of investigative dermatology.

[12]  Takeshi Yasui,et al.  In vivo observation of age-related structural changes of dermal collagen in human facial skin using collagen-sensitive second harmonic generation microscope equipped with 1250-nm mode-locked Cr:Forsterite laser , 2012, Journal of biomedical optics.

[13]  O. Urakawa,et al.  Dielectric relaxation of guest molecules in a clathrate structure of syndiotactic polystyrene. , 2012, The journal of physical chemistry. B.

[14]  R. Timpl.  Structure and biological activity of basement membrane proteins. , 1989, European journal of biochemistry.

[15]  Horst Wenck,et al.  Impact of collagen crosslinking on the second harmonic generation signal and the fluorescence lifetime of collagen autofluorescence , 2012, Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging.

[16]  Michel Manfait,et al.  Combination of FTIR spectral imaging and chemometrics for tumour detection from paraffin-embedded biopsies. , 2008, The Analyst.

[17]  G. Gouspillou,et al.  Collagen types analysis and differentiation by FTIR spectroscopy , 2009, Analytical and bioanalytical chemistry.

[18]  James Varani,et al.  Decreased collagen production in chronologically aged skin: roles of age-dependent alteration in fibroblast function and defective mechanical stimulation. , 2006, The American journal of pathology.

[19]  Michel Manfait,et al.  Differential diagnosis of cutaneous carcinomas by infrared spectral micro-imaging combined with pattern recognition. , 2009, The Analyst.

[20]  R. Lavker,et al.  Structural alterations in exposed and unexposed aged skin. , 1979, The Journal of investigative dermatology.

[21]  Frank A. Robey,et al.  Long-term sun exposure alters the collagen of thepapillary dermis , 1996 .

[22]  Christoph Krafft,et al.  Disease recognition by infrared and Raman spectroscopy , 2009, Journal of biophotonics.

[23]  Benjamin Bird,et al.  Infrared micro-spectral imaging: distinction of tissue types in axillary lymph node histology , 2008, BMC clinical pathology.

[24]  In situ three-dimensional monitoring of collagen fibrillogenesis using SHG microscopy , 2012, Biomedical optics express.

[25]  R Mendelsohn,et al.  FTIR microscopic imaging of collagen and proteoglycan in bovine cartilage. , 2001, Biopolymers.

[26]  D. Wurster,et al.  Investigation of normal and acne skin surface lipids. , 1969, Journal of pharmaceutical sciences.

[27]  S. Shuster,et al.  The influence of age and sex on skin thickness, skin collagen and density , 1975, The British journal of dermatology.

[28]  Max Diem,et al.  Immunohistochemistry, histopathology and infrared spectral histopathology of colon cancer tissue sections , 2013, Journal of biophotonics.

[29]  R. Mendelsohn,et al.  Determination of molecular conformation and permeation in skin via IR spectroscopy, microscopy, and imaging. , 2006, Biochimica et biophysica acta.

[30]  Max Diem,et al.  Artificial neural networks as supervised techniques for FT‐IR microspectroscopic imaging , 2006, Journal of chemometrics.

[31]  Hui Li,et al.  Quantitative analysis on collagen morphology in aging skin based on multiphoton microscopy. , 2011, Journal of biomedical optics.

[32]  J. Voorhees,et al.  c-Jun-dependent inhibition of cutaneous procollagen transcription following ultraviolet irradiation is reversed by all-trans retinoic acid. , 2000, The Journal of clinical investigation.

[33]  A M Rubenchik,et al.  Collagen structure and nonlinear susceptibility: Effects of heat, glycation, and enzymatic cleavage on second harmonic signal intensity , 2000, Lasers in surgery and medicine.

[34]  J. Herzfeld,et al.  Altered hydrogen bonding of Arg82 during the proton pump cycle of bacteriorhodopsin: a low-temperature polarized FTIR spectroscopic study. , 2004, Biochemistry.