Isolation of human periosteum-derived progenitor cells using immunophenotypes for chondrogenesis

Periosteum-derived progenitor cells (PDPCs) were isolated by characteristic surface markers. Reproducibility of immunophenotypes of the PDPCs was characterized by flow cytometric analysis using fluorescence-activated cell sorter (FACS). SH2+, SH3+, SH4+, CD9+, CD90+ and CD105+ were important eternal characteristic cell surface markers for the PDPCs. The characterized PDPCs maintained their chondrogenic potential in pellet cultures until the 15th passage from primary cell culture.

[1]  S W O'Driscoll,et al.  The role of periosteum in cartilage repair. , 2001, Clinical orthopaedics and related research.

[2]  F. Luyten,et al.  Human periosteum-derived cells maintain phenotypic stability and chondrogenic potential throughout expansion regardless of donor age. , 2001, Arthritis and rheumatism.

[3]  Joseph Zaia,et al.  Mesenchymal Stem Cell Surface Antigen SB‐10 Corresponds to Activated Leukocyte Cell Adhesion Molecule and Is Involved in Osteogenic Differentiation , 1998, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.

[4]  A I Caplan,et al.  Characterization of cells with osteogenic potential from human marrow. , 1992, Bone.

[5]  S. Hwang,et al.  In vitro mesengenic potential of human umbilical cord blood-derived mesenchymal stem cells. , 2004, Biochemical and biophysical research communications.

[6]  S. O’Driscoll,et al.  Localization of chondrocyte precursors in periosteum. , 2001, Osteoarthritis and cartilage.

[7]  R. Deans,et al.  Mesenchymal stem cells: biology and potential clinical uses. , 2000, Experimental hematology.

[8]  E A Morris,et al.  Isolation, characterization, and chondrogenic potential of human bone marrow‐derived multipotential stromal cells , 2000, Journal of cellular physiology.

[9]  R. Brenner,et al.  Identification, quantification and isolation of mesenchymal progenitor cells from osteoarthritic synovium by fluorescence automated cell sorting. , 2003, Osteoarthritis and cartilage.