Assessment of carotenoids, capsaicinoids and ascorbic acid composition of some selected pepper cultivars (Capsicum annuum L.) grown in Turkey

Abstract Carotenoid, capsaicinoid and ascorbic acid composition of ripe fruits of five Capsicum annuum cultivars (730 F1, 1245 F1, Amazon F1, Serademre 8 and Kusak 295F1), grown as principle breeding material in Turkey, were quantitatively investigated by means of HPLC technique. Seven main carotenoids, five analogues of capsaicinoids and ascorbic acid were quantified in the fruits grown for 2 year replication. From the capsaicinoids and carotenoids data, Scoville Heat Unit (SHU) and retinol activity equivalent (RAE) values of the fruits were also calculated, respectively. The findings determined that the cultivars of 730 F1 and 1245 F1 had higher carotenoids (2310–2390 mg/kg in dry basis), capsaicinoids (471.3–688.1 mg/kg in dry basis), vitamin A (218.8–243.0 μg RAE/100 g in wet basis) and vitamin C (63.1–64.9 mg/100 g in wet basis) content, without any significant difference among each of them. Furthermore, the cultivars which had higher capsaicinoids contents had higher ascorbic acids content as well. With their high nutritional and functional components, the cultivar of 730 F1 and 1245 F1 can be considered to be selected breeding material for cultivar development.

[1]  Junsoo Park,et al.  Composition of Main Carotenoids in Korean Red Pepper (Capsicum annuum, L) and Changes of Pigment Stability During the Drying and Storage Process , 2004 .

[2]  L. Howard,et al.  Carotenoids in pungent and non‐pungent peppers at various developmental stages grown in the field and glasshouse , 2002 .

[3]  R. Gómez-Ladrón de Guevara,et al.  Carotenoid biosynthesis changes in five red pepper (Capsicum annuum L.) cultivars during ripening. Cultivar selection for breeding. , 2000, Journal of agricultural and food chemistry.

[4]  A. Krajewska,et al.  Sensory Properties of Naturally Occurring Capsaicinoids , 1988 .

[5]  Paul W. Bosland,et al.  Improved method for quantifying capsaicinoids in Capsicum using high-performance liquid chromatography. , 1995 .

[6]  T. Biftu,et al.  DETERMINATION OF PUNGENCY DUE TO CAPSICUM BY GAS‐LIQUID CHROMATOGRAPHY , 1977 .

[7]  P. Trumbo,et al.  Dietary reference intakes: vitamin A, vitamin K, arsenic, boron, chromium, copper, iodine, iron, manganese, molybdenum, nickel, silicon, vanadium, and zinc. , 1998, Journal of the American Dietetic Association.

[8]  E. Simonne,et al.  Ascorbic Acid and Provitamin A Contents in Unusually Colored Bell Peppers (Capsicum annuumL.) , 1997 .

[9]  A. Gálvez,et al.  Study of Lability and Kinetics of the Main Carotenoid Pigments of Red Pepper in the De-esterification Reaction. , 1998 .

[10]  P. Bosland.  Chiles: A Diverse Crop , 1992 .

[11]  J. Olson.  Biological actions of carotenoids. , 1989, The Journal of nutrition.

[12]  M. Materska,et al.  Phenylalanine ammonia-lyase and antioxidant activities of lipophilic fraction of fresh pepper fruits Capsicum annum L. , 2001 .

[13]  K. Weaver,et al.  Rapid high-performance liquid chromatographic method for the determination of very low capsaicin levels. , 1986, Journal of chromatography.

[14]  H. Daood,et al.  Change in the carotenoid and antioxidant content of spice red pepper (paprika) as a function of ripening and some technological factors. , 1999, Journal of agricultural and food chemistry.

[15]  Corinne H Robinson,et al.  Normal and Therapeutic Nutrition , 1962 .

[16]  J. Bertram,et al.  Carotenoids enhance gap junctional communication and inhibit lipid peroxidation in C3H/10T1/2 cells: relationship to their cancer chemopreventive action. , 1991, Carcinogenesis.

[17]  J. Kanner,et al.  Hydrolysis study of carotenoid pigments of paprika (Capsicum annuum L. variety Lehava) by HPLC/photodiode array detection , 1993 .

[18]  M. Petz,et al.  Extraction of capsaicinoids from chillies (Capsicum frutescens L.) and paprika (Capsicum annuum L.) using supercritical fluids and organic solvents , 1997 .

[19]  J. Kanner,et al.  Carotenoid Pigments and .beta.-Carotene in Paprika Fruits (Capsicum spp.) with Different Genotypes , 1995 .

[20]  M. Wall.  Ascorbic acid, vitamin A, and mineral composition of banana (Musa sp.) and papaya (Carica papaya) cultivars grown in Hawaii , 2006 .

[21]  K. Scott,et al.  Pro-vitamin A carotenoid conversion factors: retinol equivalents - fact or fiction? , 2000 .

[22]  D. E. Henderson,et al.  Quantitative HPLC determination of the antioxidant activity of capsaicin on the formation of lipid hydroperoxides of linoleic acid: a comparative study against BHT and melatonin. , 1999, Journal of agricultural and food chemistry.

[23]  E. Yahia,et al.  Changes in capsaicinoids during development, maturation and senescence of chile peppers and relation with peroxidase activity , 1998 .

[24]  B. Frei,et al.  Can antioxidant vitamins materially reduce oxidative damage in humans? , 1999, Free radical biology & medicine.

[25]  D. Hornero-Méndez,et al.  Separation and quantification of the carotenoid pigments in red peppers (Capsicum annuum L.), paprika, and oleoresin by reversed-phase HPLC , 1993 .

[26]  L. Dorantes,et al.  Inhibition of growth of some foodborne pathogenic bacteria by Capsicum annum extracts , 2000 .

[27]  T. G. Truscott,et al.  The carotenoids as anti-oxidants--a review. , 1997, Journal of photochemistry and photobiology. B, Biology.

[28]  C. Weisskopf,et al.  Simple Method for Quantitation of Capsaicinoids in Peppers Using Capillary Gas Chromatography , 1998 .

[29]  Ayhan Topuz,et al.  Influences of gamma irradiation and storage on the capsaicinoids of sun-dried and dehydrated paprika , 2004 .

[30]  M. Mínguez-Mosquera,et al.  Influence of the industrial drying processes of pepper fruits (Capsicum annuum cv. Bola) for paprika on the carotenoid content , 1994 .

[31]  Ayhan Topuz,et al.  Influences of γ-Irradiation and Storage on the Carotenoids of Sun-Dried and Dehydrated Paprika , 2003 .

[32]  J. V. van Zanden,et al.  The pro-oxidant chemistry of the natural antioxidants vitamin C, vitamin E, carotenoids and flavonoids. , 2002, Environmental toxicology and pharmacology.

[33]  S. Simsek,et al.  Potential Application of Hot Rehydration Alone or in Combination with Hydrogen Peroxide to Control Pectin Methylesterase Activity and Microbial Load in Cold-stored Intermediate-moisture Sun-dried Figs , 2006 .

[34]  W. Robbins.  Clinical Applications of Capsaicinoids , 2000, The Clinical journal of pain.

[35]  D. Hornero-Méndez,et al.  Comparative Study of the Effect of Paprika Processing on the Carotenoids in Peppers (Capsicum annuum) of the Bola and Agridulce Varieties , 1994 .

[36]  H. Tsuchiya,et al.  Biphasic membrane effects of capsaicin, an active component in Capsicum species. , 2001, Journal of ethnopharmacology.

[37]  E. E. Burns,et al.  Provitamin A and Ascorbic Acid Content of Fresh Pepper Cultivars (Capsicum annuum) and Processed Jalapeños , 1994 .

[38]  M. Mínguez-Mosquera,et al.  Formation and Transformation of Pigments during the Fruit Ripening of Capsicum annuum Cv. Bola and Agridulce , 1994 .

[39]  C. Chichester,et al.  Metabolism and nutritional significance of carotenoids. , 1981, Annual review of nutrition.

[40]  C. Richardson.  Long-term opioid therapy for refractory lumbar pain. Pain Research Foundation journal club. , 2000, The Clinical journal of pain.

[41]  W. Oleszek,et al.  Extraction and determination of capsaicinoids in fruit of hot pepper Capsicum annuum L. by spectrophotometry and high-performance liquid chromatography , 2000 .

[42]  Impact of the increased thermal processing on retinol equivalent values of paprika oleoresins , 2005 .