Microfluidics is one of the most fascinating fields that researchers have been trying to apply in a large number of scientific disciplines over the past two decades. Among them, the discipline of food and pharmaceutical formulation encountered several obstacles when combined microfluidics and aqueous media. Indeed the physical properties of liquids at micrometric volumes being particular, the droplets generation within microfluidic devices is a big challenge to be met. This focus review is intended to be an initiation for whom would like to generate microdroplets in microfluidic systems involving continuous-aqueous phases. It provides a state-of-the-art of such systems while focusing on the microfluidic devices used, their applications to form a wide variety of emulsions and particles as well as the key role held by the interface between the device channels and the emulsion. The review also leads to reflections on new materials that can be used in microfluidic systems with aqueous-continuous phases.