N- and C-terminal region mediated oligomerization of the cyclodextrin-/pullulan degrading enzymes

,J.W.,N-and C-terminal region mediated oligomerization of the cyclodextrin-/pullulandegrading enzymes. Biologia, Bratislava, 57/Suppl. 11: 87|92, 2002; ISSN0006-3088.Cyclodextrin-/pullulan (CD-/PUL)-degrading enzymes catalyze hydrolysisand transglycosylation reactions of various substrates such as starch, cy-clodextrin, and pullulan. Recently, these enzymes have been proved to existin equilibria of monomer-dimer and monomer-dimer-tetramer or -dodecamer.Two regions in the CD-/PUL-degrading enzymes were identi ed as being in-volved in oligomerization; one is close to the N-terminal and the other locatednear the C-terminal region. The three-dimensional structure and deletion mu-tagenesis analyses revealed that the N-terminal region a ected the dimeriza-tion properties of monomeric Thermus maltogenic amylase (ThMA). On theother hand, both the N- and C-terminal regions were involved in dodecamer-ization of cyclodextrinase I-5 (CDase I-5) dimeric units. Oligomerization ofthese enzymes was also modulated by salt concentration and pH of the reac-tion bu er.Key words: cyclodextrin degrading enzyme, dimer, dodecamer, oligomericstate.Abbreviations: CD, cyclodextrin; CDase, cyclodextrinase; PUL, pullulan;ThMA, Thermus maltogenic amylase; TVAII, Thermoactinomyces vulgarisalpha-amylase II.

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