Measurement error (ME) in self‐reported sugars may be obscuring the true association of sugars with disease risk in epidemiologic studies. We aimed to assess performance of 3 self‐report methods to measure TS relative to 24‐h urinary sucrose and fructose, previously reported as predictive biomarker of TS intake. The Nutrition and Physical Activity Assessment Study (NPAAS) is a biomarker study within Women’s Health Initiative Observational Study including 450 post‐menopausal women aged 60‐91 during NPAAS. FFQ, 4DFR and three 24HDRs, along with urinary TS biomarker were collected. The biomarker, calibrated based on ME parameters previously estimated, was used as a measure of TS intake. Performance (% variation explained) of the 3 self‐report methods to measure TS was assessed by regressing calibrated biomarker on FFQ‐, 4DFR‐ or 24HDR‐based TS, adjusting for participants’ characteristics. Across all methods, self‐reported intake explained only small fraction of variation in biomarker‐predicted TS (Table 1). Age, BMI, smoking, education and physical activity somewhat improved predictability of the model, yet left majority (73‐88%) of variation unexplained. Based on the biomarker, none of self‐reports provided good estimate of intake. Measuring a sugars biomarker in a subset in epidemiologic studies may be necessary to yield more reliable intake, hence disease risk estimates for sugars.