Nuclear localization of the protein from the open reading frame x1 of the Borna disease virus was through interactions with the viral nucleoprotein.

Previous studies have predicted the presence of a small open reading frame (ORFx1) located between ORF-1 and ORF-2 of the Borna disease viral (BDV) genome. The ORFx1 is expressed as a p10 protein that is localized in the nucleus and cytoplasm of BDV-infected cells. In this study, we cloned the nucleotide sequence of ORFx1 into expression vectors and showed that it is expressed as p10. An anti-p10 serum gave nuclear and cytoplasmic staining of cells persistently infected with BDV. Immunoprecipitation of p10 from BDV-infected cells coprecipitated the p40 nucleoprotein N and the 24-kDa viral phosphoprotein P. Transient transfection of noninfected cells showed that p10 and p40 can be coprecipitated and revealed that p10 localized in the cytoplasm was imported into the nucleus in the presence of the BDV p40 N. In vitro protein-protein interaction studies on solid phase showed the direct interaction of the p10 with the BDV N protein. The subcellular distribution of p10 and its interaction with p40 suggest that this protein may play a role in the nuclear replication and/or transcription of BDV.

[1]  M. Salvatore,et al.  Interactions of the Borna Disease Virus P, N, and X Proteins and Their Functional Implications* , 1998, The Journal of Biological Chemistry.

[2]  K. Ikuta,et al.  Nuclear targeting activity associated with the amino terminal region of the Borna disease virus nucleoprotein. , 1998, Virology.

[3]  J. Richt,et al.  Detection of a novel Borna disease virus-encoded 10 kDa protein in infected cells and tissues. , 1997, The Journal of general virology.

[4]  J. Pyper,et al.  Molecular basis for the differential subcellular localization of the 38- and 39-kilodalton structural proteins of Borna disease virus , 1997, Journal of virology.

[5]  J. C. de la Torre,et al.  Sequence characterization of human Borna disease virus. , 1996, Virus research.

[6]  R. Dürrwald,et al.  First isolates of infectious human Borna disease virus from patients with mood disorders. , 1996, Molecular psychiatry.

[7]  K. Ikuta,et al.  Sequence variability of Borna disease virus open reading frame II found in human peripheral blood mononuclear cells , 1996, Journal of virology.

[8]  M. Billeter,et al.  Rescue of measles viruses from cloned DNA. , 1995, The EMBO journal.

[9]  R. Lamb,et al.  The remarkable coding strategy of borna disease virus: a new member of the nonsegmented negative strand RNA viruses. , 1995, Virology.

[10]  M. Whitt,et al.  Recombinant vesicular stomatitis viruses from DNA. , 1995, Proceedings of the National Academy of Sciences of the United States of America.

[11]  W. Zimmermann,et al.  Borna disease virus genome transcribed and expressed in psychiatric patients , 1995, Nature Medicine.

[12]  G. Gosztonyi,et al.  Borna disease--neuropathology and pathogenesis. , 1995, Current topics in microbiology and immunology.

[13]  J. C. Torre,et al.  Molecular biology of borna disease virus: prototype of a new group of animal viruses. , 1994 .

[14]  S. Rubin,et al.  Borna disease virus p24 and p38/40 synthesized in a baculovirus expression system: virus protein interactions in insect and mammalian cells. , 1994, Virology.

[15]  L. Stitz,et al.  Characterization of a Borna disease virus glycoprotein, gp18 , 1994, Journal of virology.

[16]  J. Valcárcel,et al.  RNA splicing contributes to the generation of mature mRNAs of Borna disease virus, a non-segmented negative strand RNA virus. , 1994, Virus research.

[17]  K. Conzelmann,et al.  Infectious rabies viruses from cloned cDNA. , 1994, The EMBO journal.

[18]  W. Lipkin,et al.  RNA splicing in Borna disease virus, a nonsegmented, negative-strand RNA virus , 1994, Journal of virology.

[19]  T. Briese,et al.  Genomic organization of Borna disease virus. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[20]  J. C. de la Torre,et al.  Borna disease virus (BDV), a nonsegmented RNA virus, replicates in the nuclei of infected cells where infectious BDV ribonucleoproteins are present , 1994, Journal of virology.

[21]  L. De,et al.  Sequence and genome organization of Borna disease virus , 1994, Journal of virology.

[22]  F. Steinbach,et al.  A novel marker for Borna disease virus infection , 1994, The Lancet.

[23]  J. Clements,et al.  Genomic organization of the structural proteins of borna disease virus revealed by a cDNA clone encoding the 38-kDa protein. , 1993, Virology.

[24]  D. S. Lee,et al.  Biology and neurobiology of Borna disease viruses (BDV), defined by antibodies, neutralizability and their pathogenic potential. , 1993, Archives of virology. Supplementum.

[25]  J. Amsterdam,et al.  Detection of Borna disease virus-reactive antibodies from patients with affective disorders by western immunoblot technique. , 1993, Journal of affective disorders.

[26]  T. Briese,et al.  Borna disease virus, a negative-strand RNA virus, transcribes in the nucleus of infected cells. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[27]  L. Stitz,et al.  The 24K protein of Borna disease virus. , 1992, Journal of General Virology.

[28]  W. Lipkin,et al.  Molecular characterization of the Borna disease agent. , 1990, Virology.

[29]  J. Clements,et al.  A borna virus cDNA encoding a protein recognized by antibodies in humans with behavioral diseases. , 1990, Science.

[30]  J. Amsterdam,et al.  Borna Disease Virus: A Possible Etiologic Factor in Human Affective Disorders? , 1985 .

[31]  J. Amsterdam,et al.  Detection of serum antibodies to Borna disease virus in patients with psychiatric disorders. , 1985, Science.

[32]  F. Sanger,et al.  DNA sequencing with chain-terminating inhibitors. , 1977, Proceedings of the National Academy of Sciences of the United States of America.